Restriction map for
pFN21AE5323
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BanI
BanII BbeI BbrPI BbsI BcgI
BcnI BfrI BglI BglII BlnI
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoT22I FauI FseI FspI
HaeII HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AciI 469 CCGC CCGC(-3/-1)
AlwNI 368 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
BamHI 344 GGATCC G^GATCC
BbvCI 595 CCTCAGC CCTCAGC(-5/-2)
BciVI 211 GTATCC GTATCC(6/5)
BclI 738 TGATCA T^GATCA
BfaI 356 CTAG C^TAG
BlpI 514 GCTCAGC GC^T[ACGT]AGC
BmyI 68 GTGCCC G[AGT]GC[ACT]^C
Bpu1102I 514 GCTCAGC GC^T[ACGT]AGC
BsaJI 472 CCTTGG C^C[ACGT][ACGT]GG
BseRI 692 CTCCTC GAGGAG(10/8)
BsmAI 702 GTCTC GTCTC(1/5)
BsmFI 463 GTCCC GGGAC(10/14)
BsmI 839 GCATTC GAATGC(1/-1)
Bsp1286I 68 GTGCCC G[AGT]GC[ACT]^C
BstYI 344 GGATCC [GA]^GATC[CT]
CelII 514 GCTCAGC GC^T[ACGT]AGC
CfoI 799 GCGC GCG^C
EarI 609 CTCTTC CTCTTC(1/4)
EcoRV 404 GATATC GAT^ATC
EcoT14I 472 CCTTGG C^C[AT][AT]GG
FbaI 738 TGATCA T^GATCA
HhaI 799 GCGC GCG^C
HinP1I 799 GCGC G^CGC
HinfI 10 GATTC G^A[ACGT]TC
Ksp632I 609 CTCTTC CTCTTC(1/4)
MaeI 356 CTAG C^TAG
MaeII 483 ACGT A^CGT
MflI 344 GGATCC [GA]^GATC[CT]
MspA1I 371 CAGCTG C[AC]G^C[GT]G
NlaIII 742 CATG CATG^
PvuII 371 CAGCTG CAG^CTG
SexAI 764 ACCAGGT A^CC[AT]GGT
StyI 472 CCTTGG C^C[AT][AT]GG
TfiI 10 GATTC G^A[AT]TC
XhoII 344 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcsI 151 AAATTT [GA]^AATT[CT]
263 AAATTT
ApoI 151 AAATTT [GA]^AATT[CT]
263 AAATTT
AvaII 316 GGTCC G^G[AT]CC
462 GGTCC
BbvI 370 GCAGC GCAGC(8/12)
810 GCAGC
BpmI 446 CTCCAG CTGGAG(16/14)
206 CTGGAG
BspMI 534 ACCTGC ACCTGC(4/8)
629 ACCTGC
BstXI 466 CCACCGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
571 CCAGGACAGTGG
Cac8I 366 GCCAGC GC[ACGT]^[ACGT]GC
795 GCCTGC
Cfr13I 316 GGTCC G^G[ACGT]CC
462 GGTCC
Fnu4HI 370 GCAGC GC^[ACGT]GC
810 GCAGC
HaeIII 476 GGCC GG^CC
552 GGCC
HphI 40 TCACC GGTGA(8/7)
762 TCACC
ItaI 370 GCAGC GC^[ACGT]GC
810 GCAGC
MslI 421 CATAATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
786 CATCACTATG
NlaIV 344 GGATCC GG[ACGT]^[ACGT]CC
462 GGTCCC
PflMI 73 CCAAGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
367 CCAGCAGCTGG
Sau96I 316 GGTCC G^G[ACGT]CC
462 GGTCC
TseI 370 GCAGC G^C[AT]GC
810 GCAGC
Tsp45I 39 GTCAC ^GT[GC]AC
232 GTCAC
Van91I 73 CCAAGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
367 CCAGCAGCTGG
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI AlwI BsiYI BslI BsrI
BssKI BstF5I BstNI DdeI DpnII
EcoRII FokI MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
Sau3AI ScrFI SfaNI Tru9I Tsp509I
TspRI