Restriction map for
pFN21AE9523
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BssHII BstAPI BstBI BstEII BstPI
BstXI CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HhaI Hin1I HinP1I
HpaII KasI KpnI KspI MaeII
MroI MspA1I MspI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SwaI TaqI
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 803 GTATAC GT^[AC][GT]AC
AccII 940 CGCG CG^CG
AciI 976 GCGG CCGC(-3/-1)
AflIII 939 ACGCGT A^C[GA][CT]GT
AocI 82 CCTGAGG CC^T[ACGT]AGG
AvaII 1285 GGTCC G^G[AT]CC
BalI 593 TGGCCA TGG^CCA
BanI 1023 GGTGCC G^G[CT][GA]CC
BbsI 405 GAAGAC GAAGAC(2/6)
BglII 308 AGATCT A^GATCT
BpuAI 405 GAAGAC GAAGAC(2/6)
BsaBI 859 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1137 GGTCTC GGTCTC(1/5)
BsgI 410 CTGCAC GTGCAG(16/14)
BsiYI 1027 CCATTGCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1027 CCATTGCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 1138 GTCTC GTCTC(1/5)
BsmFI 642 GTCCC GGGAC(10/14)
Bsp1407I 989 TGTACA T^GTACA
BsrGI 989 TGTACA T^GTACA
BssSI 394 CACGAG CACGAG(-5/-1)
Bst1107I 803 GTATAC GTA^TAC
BstUI 940 CGCG CG^CG
BstYI 308 AGATCT [GA]^GATC[CT]
BstZ17I 803 GTATAC GTA^TAC
Bsu36I 82 CCTGAGG CC^T[ACGT]AGG
Cac8I 346 GCAAGC GC[ACGT]^[ACGT]GC
Eco81I 82 CCTGAGG CC^T[ACGT]AGG
HgaI 1255 GCGTC GACGC(5/10)
HindIII 822 AAGCTT A^AGCTT
HpaI 385 GTTAAC GTT^AAC
MamI 859 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 840 CAATTG C^AATTG
MflI 308 AGATCT [GA]^GATC[CT]
MluI 939 ACGCGT A^CGCGT
MluNI 593 TGGCCA TGG^CCA
MscI 593 TGGCCA TGG^CCA
MunI 840 CAATTG C^AATTG
MvnI 940 CGCG CG^CG
NlaIV 1023 GGTGCC GG[ACGT]^[ACGT]CC
NspI 807 ACATGC [GA]CATG^[CT]
PflMI 1027 CCATTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 587 CTGCAG CTGCA^G
SapI 1243 GCTCTTC GCTCTTC(1/4)
SfcI 587 CTGCAG C^T[GA][CT]AG
SspBI 989 TGTACA T^GTACA
SspI 931 AATATT AAT^ATT
StuI 605 AGGCCT AGG^CCT
Tsp45I 314 GTGAC ^GT[GC]AC
Van91I 1027 CCATTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 308 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwI 286 GATCC GGATC(4/5)
848 GGATC
AlwNI 198 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
239 CAGGAACTG
Asp700I 1240 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1348 GAAAATGTTC
BseRI 868 CTCCTC GAGGAG(10/8)
1084 GAGGAG
BspHI 122 TCATGA T^CATGA
1299 TCATGA
BsrDI 813 CATTGC GCAATG(2/0)
1028 CATTGC
Cfr13I 886 GGCCC G^G[ACGT]CC
1285 GGTCC
DsaI 882 CCATGG C^C[GA][CT]GG
1057 CCATGG
EaeI 593 TGGCCA [CT]^GGCC[GA]
728 TGGCCG
EarI 1244 CTCTTC CTCTTC(1/4)
73 GAAGAG
EcoT14I 882 CCATGG C^C[AT][AT]GG
1057 CCATGG
HincII 385 GTTAAC GT[CT]^[GA]AC
1269 GTCAAC
HindII 385 GTTAAC GT[CT]^[GA]AC
1269 GTCAAC
Ksp632I 1244 CTCTTC CTCTTC(1/4)
73 GAAGAG
NcoI 882 CCATGG C^CATGG
1057 CCATGG
PleI 88 GACTC GAGTC(4/5)
78 GAGTC
RcaI 122 TCATGA T^CATGA
1299 TCATGA
Sau96I 886 GGCCC G^G[ACGT]CC
1285 GGTCC
StyI 882 CCATGG C^C[AT][AT]GG
1057 CCATGG
XmnI 1240 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1348 GAAAATGTTC
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BfaI BsaJI BsrI BssKI BstF5I
BstNI DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NdeII
NlaIII RsaI Sau3AI ScrFI SfaNI
TfiI Tru9I TseI Tsp509I TspRI