Restriction map for
pFN21AE6182
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# NO CUT ENZYMES ( 196 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FokI
FseI FspI HaeII HapII HgaI
Hin1I HincII HindII HindIII HinfI
HpaI HpaII HphI KasI KpnI
Ksp632I KspI MaeII MaeIII MamI
MboII MfeI MluI MluNI MroI
MscI MslI MspI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcsI 220 AAATTT [GA]^AATT[CT]
ApaI 140 GGGCCC GGGCC^C
ApoI 220 AAATTT [GA]^AATT[CT]
Asp700I 314 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 49 GGATCC G^GATCC
BfaI 104 CTAG C^TAG
BpmI 113 CTGGAG CTGGAG(16/14)
BsaI 382 GGTCTC GGTCTC(1/5)
BsaJI 354 CCTGGG C^C[ACGT][ACGT]GG
BspMI 232 GCAGGT ACCTGC(4/8)
BssKI 354 CCTGG ^CC[ACGT]GG
BstAPI 232 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 354 CCTGG CC^[AT]GG
BstYI 49 GGATCC [GA]^GATC[CT]
CfoI 11 GCGC GCG^C
DdeI 158 CTCAG C^T[ACGT]AG
DraII 139 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 139 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRII 354 CCTGG ^CC[AT]GG
HaeIII 141 GGCC GG^CC
HhaI 11 GCGC GCG^C
HinP1I 11 GCGC G^CGC
MaeI 104 CTAG C^TAG
MflI 49 GGATCC [GA]^GATC[CT]
MseI 134 TTAA T^TAA
MspA1I 334 CAGCTG C[AC]G^C[GT]G
MvaI 354 CCTGG CC^[AT]GG
NdeI 344 CATATG CA^TATG
NlaIII 423 CATG CATG^
PvuII 334 CAGCTG CAG^CTG
ScrFI 354 CCTGG CC^[ACGT]GG
SfaNI 79 GCATC GCATC(5/9)
TaqI 93 TCGA T^CGA
Tru9I 134 TTAA T^TAA
TthHB8I 93 TCGA T^CGA
XhoII 49 GGATCC [GA]^GATC[CT]
XmnI 314 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwNI 233 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
331 CAGCAGCTG
BanII 120 GGGCTC G[GA]GC[CT]^C
140 GGGCCC
BmyI 120 GGGCTC G[AGT]GC[ACT]^C
140 GGGCCC
BsgI 24 CTGCAC GTGCAG(16/14)
230 GTGCAG
Bsp1286I 120 GGGCTC G[AGT]GC[ACT]^C
140 GGGCCC
BsrI 112 ACTGG ACTGG(1/-1)
185 CCAGT
Cfr13I 140 GGGCC G^G[ACGT]CC
141 GGCCC
DpnII 39 GATC ^GATC
50 GATC
MboI 39 GATC ^GATC
50 GATC
NdeII 39 GATC ^GATC
50 GATC
Sau3AI 39 GATC ^GATC
50 GATC
Sau96I 140 GGGCC G^G[ACGT]CC
141 GGCCC
Tsp509I 72 AATT ^AATT
221 AATT
TspRI 83 CAGTG CA[GC]TG[ACGT][ACGT]^
186 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AluI AlwI BbvI BsmAI Cac8I
Fnu4HI ItaI MnlI MwoI NlaIV
TseI