Restriction map for
pFN21AE5570
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AfaI AflII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MfeI
MluI MluNI MroI MscI MslI
MspA1I MspI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AclI 412 AACGTT AA^CGTT
AflIII 488 ACATGT A^C[GA][CT]GT
AhdI 397 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1175 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 397 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 609 GGTCC G^G[AT]CC
BfaI 889 CTAG C^TAG
BglII 443 AGATCT A^GATCT
BsaBI 307 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 395 GAGACC GGTCTC(1/5)
BsgI 1058 GTGCAG GTGCAG(16/14)
BsmBI 5 CGTCTC CGTCTC(1/5)
BsmFI 610 GTCCC GGGAC(10/14)
BspLU11I 488 ACATGT A^CATGT
BsrI 198 CCAGT ACTGG(1/-1)
BssSI 798 CACGAG CACGAG(-5/-1)
BstXI 198 CCAGTTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 443 AGATCT [GA]^GATC[CT]
DraII 608 AGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 966 GACAGAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 748 CGGCCA [CT]^GGCC[GA]
Eam1105I 397 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 608 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1224 GAATTC G^AATTC
FauI 984 CCCGC CCCGC(4/6)
HincII 1264 GTCAAC GT[CT]^[GA]AC
HindII 1264 GTCAAC GT[CT]^[GA]AC
MaeI 889 CTAG C^TAG
MamI 307 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 443 AGATCT [GA]^GATC[CT]
NdeI 364 CATATG CA^TATG
PpuMI 608 AGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 412 AACGTT AA^CGTT
PstI 1123 CTGCAG CTGCA^G
SfcI 1123 CTGCAG C^T[GA][CT]AG
TaqI 93 TCGA T^CGA
TthHB8I 93 TCGA T^CGA
XcmI 1130 CCATGATGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 443 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 34 AAATTT [GA]^AATT[CT]
1224 GAATTC
AlwI 262 GGATC GGATC(4/5)
312 GGATC
ApoI 34 AAATTT [GA]^AATT[CT]
1224 GAATTC
BanI 13 GGTGCC G^G[CT][GA]CC
457 GGTGCC
BbvI 424 GCTGC GCAGC(8/12)
1003 GCTGC
BpmI 521 CTCCAG CTGGAG(16/14)
778 CTGGAG
BsmI 350 GAATGC GAATGC(1/-1)
1188 GCATTC
BssKI 326 CCTGG ^CC[ACGT]GG
777 CCTGG
BstNI 326 CCTGG CC^[AT]GG
777 CCTGG
Cac8I 1125 GCAGGC GC[ACGT]^[ACGT]GC
1212 GCAAGC
DraI 616 TTTAAA TTT^AAA
942 TTTAAA
DsaI 511 CCATGG C^C[GA][CT]GG
1104 CCATGG
EcoRII 326 CCTGG ^CC[AT]GG
777 CCTGG
EcoRV 105 GATATC GAT^ATC
1153 GATATC
EcoT22I 352 ATGCAT ATGCA^T
1186 ATGCAT
Fnu4HI 424 GCTGC GC^[ACGT]GC
1003 GCTGC
ItaI 424 GCTGC GC^[ACGT]GC
1003 GCTGC
MaeII 413 ACGT A^CGT
939 ACGT
MvaI 326 CCTGG CC^[AT]GG
777 CCTGG
NcoI 511 CCATGG C^CATGG
1104 CCATGG
NsiI 352 ATGCAT ATGCA^T
1186 ATGCAT
NspI 488 ACATGT [GA]CATG^[CT]
1208 ACATGC
ScrFI 326 CCTGG CC^[ACGT]GG
777 CCTGG
SmlI 400 CTCAAG C^T[CT][GA]AG
871 CTTGAG
TfiI 387 GATTC G^A[AT]TC
1033 GAATC
TseI 424 GCTGC G^C[AT]GC
1003 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI BsaJI BseRI BsiYI
BslI BsmAI BstF5I Cfr13I DdeI
DpnII EcoT14I FokI HaeIII HinfI
HphI MaeIII MboI MboII MnlI
MseI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I SfaNI StyI
Tru9I Tsp45I Tsp509I TspRI