Restriction map for
pFN21AE2156
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbvCI BbvI BcgI BciVI
BclI BfrI BglI BlnI BlpI
BpmI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspMI
BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
BstXI Bsu36I CelII CfoI Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV FbaI Fnu4HI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI HphI ItaI KasI
KpnI KspI MamI MluI MluNI
MroI MscI MslI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I SspBI SspI StuI
SwaI TseI Tsp45I Tth111I Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 162 GTATAC GT^[AC][GT]AC
AlwNI 718 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 912 ATTAAT AT^TAAT
AsnI 912 ATTAAT AT^TAAT
AspHI 758 GTGCTC G[AT]GC[AT]^C
AvaI 902 CCCGGG C^[CT]CG[GA]G
BbrPI 707 CACGTG CAC^GTG
BbsI 1070 GAAGAC GAAGAC(2/6)
BmyI 758 GTGCTC G[AGT]GC[ACT]^C
BpuAI 1070 GAAGAC GAAGAC(2/6)
BsaAI 707 CACGTG [CT]AC^GT[GA]
BseRI 458 GAGGAG GAGGAG(10/8)
BsgI 47 GTGCAG GTGCAG(16/14)
BsiEI 650 CGACCG CG[GA][CT]^CG
BsiHKAI 758 GTGCTC G[AT]GC[AT]^C
BsoBI 902 CCCGGG C^[CT]CG[GA]G
Bsp1286I 758 GTGCTC G[AGT]GC[ACT]^C
BspHI 522 TCATGA T^CATGA
BspLU11I 499 ACATGT A^CATGT
BsrFI 1226 ACCGGC [GA]^CCGG[CT]
Bst1107I 162 GTATAC GTA^TAC
BstNI 349 CCTGG CC^[AT]GG
BstZ17I 162 GTATAC GTA^TAC
Cfr10I 1226 ACCGGC [GA]^CCGG[CT]
DsaI 753 CCATGG C^C[GA][CT]GG
EaeI 703 CGGCCA [CT]^GGCC[GA]
EarI 1195 GAAGAG CTCTTC(1/4)
EcoRII 349 CCTGG ^CC[AT]GG
EcoT14I 753 CCATGG C^C[AT][AT]GG
EcoT22I 272 ATGCAT ATGCA^T
FauI 4 GCGGG CCCGC(4/6)
HincII 249 GTCAAC GT[CT]^[GA]AC
HindII 249 GTCAAC GT[CT]^[GA]AC
HindIII 874 AAGCTT A^AGCTT
Ksp632I 1195 GAAGAG CTCTTC(1/4)
MfeI 95 CAATTG C^AATTG
MunI 95 CAATTG C^AATTG
MvaI 349 CCTGG CC^[AT]GG
NcoI 753 CCATGG C^CATGG
NheI 565 GCTAGC G^CTAGC
NlaIV 418 GGAACC GG[ACGT]^[ACGT]CC
NsiI 272 ATGCAT ATGCA^T
PleI 732 GACTC GAGTC(4/5)
PmaCI 707 CACGTG CAC^GTG
PmlI 707 CACGTG CAC^GTG
PshBI 912 ATTAAT AT^TAAT
PvuII 1189 CAGCTG CAG^CTG
RcaI 522 TCATGA T^CATGA
SmaI 902 CCCGGG CCC^GGG
SmlI 1250 CTCAAG C^T[CT][GA]AG
SpeI 1212 ACTAGT A^CTAGT
SphI 1175 GCATGC GCATG^C
StyI 753 CCATGG C^C[AT][AT]GG
TfiI 923 GATTC G^A[AT]TC
XcmI 168 CCATCAACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 902 CCCGGG C^CCGGG
XmaI 902 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 4 GCGG CCGC(-3/-1)
823 GCGG
AfaI 72 GTAC GT^AC
687 GTAC
AflIII 499 ACATGT A^C[GA][CT]GT
708 ACGTGT
AlwI 219 GATCC GGATC(4/5)
829 GATCC
BglII 126 AGATCT A^GATCT
1104 AGATCT
BsaJI 753 CCATGG C^C[ACGT][ACGT]GG
902 CCCGGG
BsiYI 451 CCAACAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
736 CCATTTCAAGG
BslI 451 CCAACAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
736 CCATTTCAAGG
BsmAI 857 GAGAC GTCTC(1/5)
1127 GTCTC
Cac8I 565 GCTAGC GC[ACGT]^[ACGT]GC
1175 GCATGC
HaeIII 704 GGCC GG^CC
751 GGCC
HinfI 732 GACTC G^A[ACGT]TC
923 GATTC
MaeII 578 ACGT A^CGT
708 ACGT
MseI 913 TTAA T^TAA
1064 TTAA
MspA1I 821 CAGCGG C[AC]G^C[GT]G
1189 CAGCTG
MwoI 867 GCTTGTGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
940 GCGATAGAGGC
NspI 499 ACATGT [GA]CATG^[CT]
1175 GCATGC
RsaI 72 GTAC GT^AC
687 GTAC
SfcI 803 CTACAG C^T[GA][CT]AG
1050 CTACAG
TaqI 513 TCGA T^CGA
1005 TCGA
Tru9I 913 TTAA T^TAA
1064 TTAA
TspRI 50 CAGTG CA[GC]TG[ACGT][ACGT]^
792 CACTG
TthHB8I 513 TCGA T^CGA
1005 TCGA
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AcsI AluI ApoI Asp700I BcnI
BfaI BsrI BssKI BstF5I BstYI
DdeI DpnII FokI HapII HpaII
MaeI MaeIII MboI MboII MflI
MnlI MspI NciI NdeII NlaIII
Sau3AI ScrFI SfaNI Tsp509I XhoII
XmnI