Restriction map for
pFN21AE3227
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BlnI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI BspDI BspEI BspHI
BspMI BsrBI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MroI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
StuI SwaI TaqI Tth111I TthHB8I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 1438 GTCTAC GT^[AC][GT]AC
AciI 108 GCGG CCGC(-3/-1)
AclI 637 AACGTT AA^CGTT
ApaI 3 GGGCCC GGGCC^C
BalI 1160 TGGCCA TGG^CCA
BamHI 992 GGATCC G^GATCC
BanII 3 GGGCCC G[GA]GC[CT]^C
BbsI 1489 GTCTTC GAAGAC(2/6)
BglI 529 GCCCGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1136 AGATCT A^GATCT
BlpI 518 GCTCAGC GC^T[ACGT]AGC
BpmI 871 CTGGAG CTGGAG(16/14)
Bpu1102I 518 GCTCAGC GC^T[ACGT]AGC
BpuAI 1489 GTCTTC GAAGAC(2/6)
BseRI 476 CTCCTC GAGGAG(10/8)
Bsp1407I 680 TGTACA T^GTACA
BsrDI 489 CATTGC GCAATG(2/0)
BsrFI 32 GCCGGC [GA]^CCGG[CT]
BsrGI 680 TGTACA T^GTACA
BstAPI 367 GCACTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 167 GGTCACC G^GT[ACGT]ACC
BstPI 167 GGTCACC G^GT[ACGT]ACC
CelII 518 GCTCAGC GC^T[ACGT]AGC
Cfr10I 32 GCCGGC [GA]^CCGG[CT]
EaeI 1160 TGGCCA [CT]^GGCC[GA]
EcoO65I 167 GGTCACC G^GT[ACGT]ACC
EcoT14I 901 CCTTGG C^C[AT][AT]GG
FauI 108 GCGGG CCCGC(4/6)
HgaI 70 GACGC GACGC(5/10)
MluNI 1160 TGGCCA TGG^CCA
MscI 1160 TGGCCA TGG^CCA
MspA1I 1494 CAGCTG C[AC]G^C[GT]G
NaeI 32 GCCGGC GCC^GGC
NgoMIV 32 GCCGGC G^CCGGC
PflMI 996 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 637 AACGTT AA^CGTT
PvuII 1494 CAGCTG CAG^CTG
ScaI 130 AGTACT AGT^ACT
SpeI 1401 ACTAGT A^CTAGT
SphI 763 GCATGC GCATG^C
SspBI 680 TGTACA T^GTACA
SspI 823 AATATT AAT^ATT
StyI 901 CCTTGG C^C[AT][AT]GG
Van91I 996 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 65 CGCG CG^CG
67 CGCG
AcsI 136 AAATTT [GA]^AATT[CT]
451 AAATTC
AlwNI 1205 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1551 CAGAAGCTG
ApoI 136 AAATTT [GA]^AATT[CT]
451 AAATTC
AvaII 89 GGACC G^G[AT]CC
1075 GGTCC
BmyI 3 GGGCCC G[AGT]GC[ACT]^C
959 GTGCCC
BsgI 27 GTGCAG GTGCAG(16/14)
82 GTGCAG
BsiYI 45 CCGGGCGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
996 CCAAGAAATGG
BslI 45 CCGGGCGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
996 CCAAGAAATGG
BsmFI 470 GGGAC GGGAC(10/14)
778 GGGAC
Bsp1286I 3 GGGCCC G[AGT]GC[ACT]^C
959 GTGCCC
BspLU11I 552 ACATGT A^CATGT
1068 ACATGT
BstUI 65 CGCG CG^CG
67 CGCG
HphI 169 TCACC GGTGA(8/7)
1350 TCACC
MaeII 23 ACGT A^CGT
638 ACGT
MvnI 65 CGCG CG^CG
67 CGCG
TfiI 606 GATTC G^A[AT]TC
1021 GAATC
XbaI 609 TCTAGA T^CTAGA
1017 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AfaI AflIII AluI AlwI BbvI
BcnI BfaI BsaJI BsmAI BsmI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII