Restriction map for
pFN21AE4195
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AvaI AviII AvrII BalI
BamHI BanI BbeI BcnI BglI
BlnI BlpI Bpu1102I BsaHI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRV EcoT22I FauI FseI
FspI HaeII Hin1I HincII HindII
HpaI KasI KpnI KspI MluI
MluNI MroI MscI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 153 GTCTAC GT^[AC][GT]AC
AciI 828 GCGG CCGC(-3/-1)
AflII 57 CTTAAG C^TTAAG
Alw44I 1713 GTGCAC G^TGCAC
ApaLI 1713 GTGCAC G^TGCAC
AspI 1492 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1147 GGACC G^G[AT]CC
BanII 1874 GAGCTC G[GA]GC[CT]^C
BbrPI 1997 CACGTG CAC^GTG
BbsI 2077 GAAGAC GAAGAC(2/6)
BbvCI 302 CCTCAGC CCTCAGC(-5/-2)
BcgI 836 CGAGGAGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1232 GGATAC GTATCC(6/5)
BclI 1502 TGATCA T^GATCA
BfrI 57 CTTAAG C^TTAAG
BpmI 1843 CTGGAG CTGGAG(16/14)
BpuAI 2077 GAAGAC GAAGAC(2/6)
BsaAI 1997 CACGTG [CT]AC^GT[GA]
BsaJI 1150 CCATGG C^C[ACGT][ACGT]GG
BsaWI 1451 TCCGGT [AT]^CCGG[AT]
Bsp1407I 286 TGTACA T^GTACA
BsrGI 286 TGTACA T^GTACA
Cac8I 226 GCACGC GC[ACGT]^[ACGT]GC
CfoI 1570 GCGC GCG^C
Cfr13I 1147 GGACC G^G[ACGT]CC
DraI 96 TTTAAA TTT^AAA
DsaI 1150 CCATGG C^C[GA][CT]GG
EcoNI 1617 CCTTTTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1150 CCATGG C^C[AT][AT]GG
FbaI 1502 TGATCA T^GATCA
HaeIII 1767 GGCC GG^CC
HapII 1452 CCGG C^CGG
HgaI 175 GCGTC GACGC(5/10)
HhaI 1570 GCGC GCG^C
HinP1I 1570 GCGC G^CGC
HpaII 1452 CCGG C^CGG
MfeI 610 CAATTG C^AATTG
MspI 1452 CCGG C^CGG
MunI 610 CAATTG C^AATTG
NcoI 1150 CCATGG C^CATGG
NlaIV 1441 GGTTCC GG[ACGT]^[ACGT]CC
NspI 866 GCATGT [GA]CATG^[CT]
PflFI 1492 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1468 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1997 CACGTG CAC^GTG
PmlI 1997 CACGTG CAC^GTG
PstI 1352 CTGCAG CTGCA^G
SacI 1874 GAGCTC GAGCT^C
Sau96I 1147 GGACC G^G[ACGT]CC
SfcI 1352 CTGCAG C^T[GA][CT]AG
SspBI 286 TGTACA T^GTACA
StyI 1150 CCATGG C^C[AT][AT]GG
TspRI 1365 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 1492 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1468 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AflIII 1996 ACACGT A^C[GA][CT]GT
1998 ACGTGT
AlwNI 167 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
995 CAGCTTCTG
BsaBI 103 GATACCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
377 GATCTTCATC
BsaI 2084 GAGACC GGTCTC(1/5)
1918 GGTCTC
BseRI 426 GAGGAG GAGGAG(10/8)
837 GAGGAG
BsmFI 975 GTCCC GGGAC(10/14)
1462 GTCCC
EcoRI 927 GAATTC G^AATTC
1533 GAATTC
HindIII 1028 AAGCTT A^AGCTT
1956 AAGCTT
MaeII 944 ACGT A^CGT
1998 ACGT
MamI 103 GATACCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
377 GATCTTCATC
NdeI 512 CATATG CA^TATG
2038 CATATG
TaqI 1044 TCGA T^CGA
2051 TCGA
TthHB8I 1044 TCGA T^CGA
2051 TCGA
XbaI 1195 TCTAGA T^CTAGA
1255 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AspHI BbvI BfaI BglII
BmyI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BspHI BssKI BstF5I BstNI
BstYI DdeI DpnII EarI EcoRII
Fnu4HI FokI HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII PleI
PvuII RcaI RsaI Sau3AI ScrFI
SfaNI SmlI TfiI Tru9I TseI
Tsp45I Tsp509I XhoII XmnI