Restriction map for
pFN21AE1696
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# NO CUT ENZYMES ( 171 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaII AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DdeI DraII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRV EcoT22I FauI FokI
FseI FspI HaeII HgaI HhaI
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeIII MamI
MfeI MflI MluI MluNI MroI
MscI MslI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XhoII
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 468 GACGTC GACGT^C
AcyI 468 GACGTC G[GA]^CG[CT]C
AfaI 459 GTAC GT^AC
AlwNI 305 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 27 GAGCTC G[AT]GC[AT]^C
AvaI 482 CCCGGG C^[CT]CG[GA]G
BanI 411 GGTGCC G^G[CT][GA]CC
BanII 27 GAGCTC G[GA]GC[CT]^C
BclI 50 TGATCA T^GATCA
BfaI 394 CTAG C^TAG
BmyI 27 GAGCTC G[AGT]GC[ACT]^C
BsaAI 460 TACGTG [CT]AC^GT[GA]
BsaHI 468 GACGTC G[GA]^CG[CT]C
BsgI 454 GTGCAG GTGCAG(16/14)
BsiHKAI 27 GAGCTC G[AT]GC[AT]^C
BsmAI 466 GAGAC GTCTC(1/5)
BsmBI 466 GAGACG CGTCTC(1/5)
BsoBI 482 CCCGGG C^[CT]CG[GA]G
Bsp1286I 27 GAGCTC G[AGT]GC[ACT]^C
BspMI 408 GCAGGT ACCTGC(4/8)
BsrBI 400 GAGCGG CCGCTC(-3/-3)
BstNI 428 CCAGG CC^[AT]GG
Cac8I 452 GCGTGC GC[ACGT]^[ACGT]GC
DpnII 51 GATC ^GATC
DraI 595 TTTAAA TTT^AAA
DraIII 43 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 442 GAAGAG CTCTTC(1/4)
EcoNI 39 CCTCCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 316 GAATTC G^AATTC
EcoRII 428 CCAGG ^CC[AT]GG
EcoT14I 32 CCTTGG C^C[AT][AT]GG
FbaI 50 TGATCA T^GATCA
HapII 483 CCGG C^CGG
Hin1I 468 GACGTC G[GA]^CG[CT]C
HpaII 483 CCGG C^CGG
HphI 48 GGTGA GGTGA(8/7)
Ksp632I 442 GAAGAG CTCTTC(1/4)
MaeI 394 CTAG C^TAG
MboI 51 GATC ^GATC
MseI 596 TTAA T^TAA
MspI 483 CCGG C^CGG
MvaI 428 CCAGG CC^[AT]GG
NdeII 51 GATC ^GATC
NlaIV 411 GGTGCC GG[ACGT]^[ACGT]CC
RsaI 459 GTAC GT^AC
SacI 27 GAGCTC GAGCT^C
Sau3AI 51 GATC ^GATC
SmaI 482 CCCGGG CCC^GGG
StyI 32 CCTTGG C^C[AT][AT]GG
TaqI 234 TCGA T^CGA
Tru9I 596 TTAA T^TAA
TspRI 365 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 234 TCGA T^CGA
XmaCI 482 CCCGGG C^CCGGG
XmaI 482 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AcsI 316 GAATTC [GA]^AATT[CT]
359 AAATTC
AluI 28 AGCT AG^CT
141 AGCT
ApoI 316 GAATTC [GA]^AATT[CT]
359 AAATTC
BbsI 121 GAAGAC GAAGAC(2/6)
477 GAAGAC
BcnI 482 CCCGG CC^[GC]GG
483 CCGGG
BpuAI 121 GAAGAC GAAGAC(2/6)
477 GAAGAC
BsaJI 32 CCTTGG C^C[ACGT][ACGT]GG
482 CCCGGG
BseRI 397 GAGGAG GAGGAG(10/8)
421 GAGGAG
HinfI 295 GAATC G^A[ACGT]TC
330 GATTC
MspA1I 63 CAGCGG C[AC]G^C[GT]G
206 CCGCTG
MwoI 62 GCAGCGGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
566 GCAAGAAAAGC
NciI 482 CCCGG CC^[GC]GG
483 CCGGG
TfiI 295 GAATC G^A[AT]TC
330 GATTC
Tsp509I 317 AATT ^AATT
360 AATT
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AciI Asp700I BbvI BsiYI BslI
BssKI Cfr13I Fnu4HI HaeIII ItaI
MaeII MboII MnlI NlaIII Sau96I
ScrFI TseI XmnI