Restriction map for
pFN21AE1652
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AfaI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI Asp718I AspEI AspHI AspI
AviII AvrII BanI BbeI BbrPI
BbsI BbvCI BcgI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaHI BsaJI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DdeI DraI
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FokI FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HphI KasI
KpnI Ksp632I KspI MaeI MaeII
MfeI MluI MroI MslI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AluI 293 AGCT AG^CT
AlwNI 158 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 79 ATTAAT AT^TAAT
AsnI 79 ATTAAT AT^TAAT
Asp700I 342 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 331 CCCGAG C^[CT]CG[GA]G
BalI 324 TGGCCA TGG^CCA
BamHI 216 GGATCC G^GATCC
BanII 334 GAGCCC G[GA]GC[CT]^C
BbvI 291 GCAGC GCAGC(8/12)
BciVI 186 GTATCC GTATCC(6/5)
BcnI 405 CCCGG CC^[GC]GG
BmyI 334 GAGCCC G[AGT]GC[ACT]^C
BpmI 11 CTCCAG CTGGAG(16/14)
BsaBI 85 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 434 GAGACC GGTCTC(1/5)
BseRI 423 CTCCTC GAGGAG(10/8)
BsiYI 64 CCTATTACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 64 CCTATTACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 17 GAGACG CGTCTC(1/5)
BsmFI 73 GGGAC GGGAC(10/14)
BsoBI 331 CCCGAG C^[CT]CG[GA]G
Bsp1286I 334 GAGCCC G[AGT]GC[ACT]^C
BstNI 13 CCAGG CC^[AT]GG
BstXI 221 CCATTTATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 376 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 137 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 324 TGGCCA [CT]^GGCC[GA]
EcoO109I 376 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 13 CCAGG ^CC[AT]GG
Fnu4HI 291 GCAGC GC^[ACGT]GC
HapII 406 CCGG C^CGG
HgaI 19 GACGC GACGC(5/10)
HpaII 406 CCGG C^CGG
ItaI 291 GCAGC GC^[ACGT]GC
MamI 85 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 306 GAAGA GAAGA(8/7)
MluNI 324 TGGCCA TGG^CCA
MscI 324 TGGCCA TGG^CCA
MspI 406 CCGG C^CGG
MvaI 13 CCAGG CC^[AT]GG
NciI 405 CCCGG CC^[GC]GG
NspI 178 ACATGC [GA]CATG^[CT]
PleI 391 GAGTC GAGTC(4/5)
PpuMI 376 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 153 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 79 ATTAAT AT^TAAT
TseI 291 GCAGC G^C[AT]GC
Tsp509I 491 AATT ^AATT
XmnI 342 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AvaII 104 GGACC G^G[AT]CC
377 GGACC
BsmAI 17 GAGAC GTCTC(1/5)
434 GAGAC
BsrDI 287 CATTGC GCAATG(2/0)
417 CATTGC
BssKI 13 CCAGG ^CC[ACGT]GG
405 CCCGG
BstYI 216 GGATCC [GA]^GATC[CT]
315 GGATCT
MflI 216 GGATCC [GA]^GATC[CT]
315 GGATCT
MseI 80 TTAA T^TAA
493 TTAA
MwoI 282 GCCCACATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
294 GCTAAAAGAGC
NlaIII 179 CATG CATG^
312 CATG
NlaIV 104 GGACCC GG[ACGT]^[ACGT]CC
216 GGATCC
ScrFI 13 CCAGG CC^[ACGT]GG
405 CCCGG
SmlI 199 CTTGAG C^T[CT][GA]AG
388 CTTGAG
Tru9I 80 TTAA T^TAA
493 TTAA
XhoII 216 GGATCC [GA]^GATC[CT]
315 GGATCT
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AlwI BsrI Cfr13I DpnII HaeIII
HinfI MaeIII MboI MnlI NdeII
Sau3AI Sau96I TfiI Tsp45I TspRI