Restriction map for
pFN21AE6668
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# NO CUT ENZYMES ( 164 enzymes) #
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AccI AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbsI BbvCI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI HinP1I HincII HindII HindIII
HpaI HpaII KasI KspI MaeIII
MamI MfeI MluI MluNI MroI
MscI MseI MslI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tru9I Tsp45I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 681 GACGTC GACGT^C
Acc65I 460 GGTACC G^GTACC
AciI 234 CCGC CCGC(-3/-1)
AcsI 611 AAATTC [GA]^AATT[CT]
AcyI 681 GACGTC G[GA]^CG[CT]C
AfaI 461 GTAC GT^AC
AlwI 93 GATCC GGATC(4/5)
AlwNI 337 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 611 AAATTC [GA]^AATT[CT]
Asp718I 460 GGTACC G^GTACC
BanI 460 GGTACC G^G[CT][GA]CC
BanII 254 GGGCTC G[GA]GC[CT]^C
BbrPI 526 CACGTG CAC^GTG
BcgI 606 GCATGAAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 311 GGATAC GTATCC(6/5)
BfaI 661 CTAG C^TAG
BmyI 254 GGGCTC G[AGT]GC[ACT]^C
BpmI 84 CTGGAG CTGGAG(16/14)
BsaAI 526 CACGTG [CT]AC^GT[GA]
BsaHI 681 GACGTC G[GA]^CG[CT]C
BseRI 124 CTCCTC GAGGAG(10/8)
BsgI 132 CTGCAC GTGCAG(16/14)
BsmAI 679 GAGAC GTCTC(1/5)
BsmBI 679 GAGACG CGTCTC(1/5)
Bsp1286I 254 GGGCTC G[AGT]GC[ACT]^C
BspHI 767 TCATGA T^CATGA
BsrDI 295 GCAATG GCAATG(2/0)
BstXI 267 CCAACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 92 AGATCC [GA]^GATC[CT]
DsaI 307 CCACGG C^C[GA][CT]GG
EaeI 259 CGGCCA [CT]^GGCC[GA]
EcoNI 142 CCTAAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 681 GACGTC G[GA]^CG[CT]C
KpnI 460 GGTACC GGTAC^C
MaeI 661 CTAG C^TAG
MflI 92 AGATCC [GA]^GATC[CT]
MspA1I 337 CAGCTG C[AC]G^C[GT]G
MwoI 388 GCTCGAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 443 ACATGC [GA]CATG^[CT]
PleI 321 GACTC GAGTC(4/5)
PmaCI 526 CACGTG CAC^GTG
PmlI 526 CACGTG CAC^GTG
PstI 61 CTGCAG CTGCA^G
PvuII 337 CAGCTG CAG^CTG
RcaI 767 TCATGA T^CATGA
RsaI 461 GTAC GT^AC
SfaNI 624 GATGC GCATC(5/9)
XhoII 92 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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BsrI 83 ACTGG ACTGG(1/-1)
750 CCAGT
DdeI 12 CTCAG C^T[ACGT]AG
335 CTCAG
DpnII 93 GATC ^GATC
121 GATC
DraII 52 GGGACCC [GA]G^G[ACGT]CC[CT]
596 GGGACCC
EcoO109I 52 GGGACCC [GA]G^G[ACGT]CC[CT]
596 GGGACCC
HphI 621 GGTGA GGTGA(8/7)
324 TCACC
MboI 93 GATC ^GATC
121 GATC
NdeII 93 GATC ^GATC
121 GATC
PpuMI 52 GGGACCC [GA]G^G[AT]CC[CT]
596 GGGACCC
Sau3AI 93 GATC ^GATC
121 GATC
TaqI 104 TCGA T^CGA
390 TCGA
TfiI 162 GATTC G^A[AT]TC
564 GATTC
TspRI 656 CAGTG CA[GC]TG[ACGT][ACGT]^
807 CACTG
TthHB8I 104 TCGA T^CGA
390 TCGA
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI AvaII BbvI BsaJI BsiYI
BslI BsmFI BssKI BstF5I BstNI
Cac8I Cfr13I EarI EcoRII Fnu4HI
FokI HaeIII HinfI ItaI Ksp632I
MaeII MboII MnlI MvaI NlaIII
NlaIV Sau96I ScrFI SfcI TseI
Tsp509I XcmI