Restriction map for
pFN21AE9413
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BbrPI
BbvCI BcgI BciVI BcnI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstXI
BstZ17I CelII ClaI DraI DraIII
DrdI EagI EclXI Eco52I EcoNI
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MunI NciI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI SacII SalI SapI SbfI
ScaI SexAI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TaqI Tru9I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 988 AAATTC [GA]^AATT[CT]
AfaI 177 GTAC GT^AC
AhdI 67 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 685 GTGCAC G^TGCAC
AlwNI 915 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 96 CCTCAGG CC^T[ACGT]AGG
Aor51HI 881 AGCGCT AGC^GCT
ApaLI 685 GTGCAC G^TGCAC
ApoI 988 AAATTC [GA]^AATT[CT]
AspEI 67 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 69 CTCGGG C^[CT]CG[GA]G
BamHI 1055 GGATCC G^GATCC
BbeI 740 GGCGCC GGCGC^C
BbsI 952 GTCTTC GAAGAC(2/6)
BclI 449 TGATCA T^GATCA
BglI 220 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 952 GTCTTC GAAGAC(2/6)
BsaI 459 GGTCTC GGTCTC(1/5)
BsmAI 460 GTCTC GTCTC(1/5)
BsoBI 69 CTCGGG C^[CT]CG[GA]G
BspLU11I 644 ACATGT A^CATGT
BsrBI 1051 GAGCGG CCGCTC(-3/-3)
BsrDI 1094 GCAATG GCAATG(2/0)
BstEII 588 GGTCACC G^GT[ACGT]ACC
BstPI 588 GGTCACC G^GT[ACGT]ACC
Bsu36I 96 CCTCAGG CC^T[ACGT]AGG
CpoI 662 CGGTCCG CG^G[AT]CCG
DsaI 134 CCGTGG C^C[GA][CT]GG
Eam1105I 67 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 814 CTCTTC CTCTTC(1/4)
Eco47III 881 AGCGCT AGC^GCT
Eco81I 96 CCTCAGG CC^T[ACGT]AGG
EcoO65I 588 GGTCACC G^GT[ACGT]ACC
FbaI 449 TGATCA T^GATCA
KasI 740 GGCGCC G^GCGCC
Ksp632I 814 CTCTTC CTCTTC(1/4)
NarI 740 GGCGCC GG^CGCC
PleI 67 GACTC GAGTC(4/5)
PstI 745 CTGCAG CTGCA^G
RsaI 177 GTAC GT^AC
RsrII 662 CGGTCCG CG^G[AT]CCG
SacI 562 GAGCTC GAGCT^C
SfiI 219 GGCCACCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 740 GGCGCC GGC^GCC
SphI 260 GCATGC GCATG^C
Tsp509I 989 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 625 GGCGTC G[GA]^CG[CT]C
740 GGCGCC
AflIII 644 ACATGT A^C[GA][CT]GT
1088 ACGTGT
ApaI 490 GGGCCC GGGCC^C
533 GGGCCC
AspHI 562 GAGCTC G[AT]GC[AT]^C
685 GTGCAC
BanI 522 GGTGCC G^G[CT][GA]CC
740 GGCGCC
BpmI 355 CTGGAG CTGGAG(16/14)
975 CTGGAG
BsaAI 583 TACGTG [CT]AC^GT[GA]
1087 TACGTG
BsaHI 625 GGCGTC G[GA]^CG[CT]C
740 GGCGCC
BsiHKAI 562 GAGCTC G[AT]GC[AT]^C
685 GTGCAC
BsrFI 186 GCCGGC [GA]^CCGG[CT]
525 GCCGGC
BstYI 101 GGATCT [GA]^GATC[CT]
1055 GGATCC
Cfr10I 186 GCCGGC [GA]^CCGG[CT]
525 GCCGGC
HapII 187 CCGG C^CGG
526 CCGG
Hin1I 625 GGCGTC G[GA]^CG[CT]C
740 GGCGCC
HpaII 187 CCGG C^CGG
526 CCGG
MflI 101 GGATCT [GA]^GATC[CT]
1055 GGATCC
MspA1I 569 CCGCTG C[AC]G^C[GT]G
748 CAGCGG
MspI 187 CCGG C^CGG
526 CCGG
NaeI 186 GCCGGC GCC^GGC
525 GCCGGC
NgoMIV 186 GCCGGC G^CCGGC
525 GCCGGC
NspI 260 GCATGC [GA]CATG^[CT]
644 ACATGT
SfcI 745 CTGCAG C^T[GA][CT]AG
855 CTACAG
StuI 791 AGGCCT AGG^CCT
998 AGGCCT
TfiI 980 GAATC G^A[AT]TC
1067 GAATC
Tsp45I 277 GTCAC ^GT[GC]AC
589 GTCAC
TspRI 156 CACTG CA[GC]TG[ACGT][ACGT]^
541 CAGTG
XhoII 101 GGATCT [GA]^GATC[CT]
1055 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccI AccII AciI AluI AlwI
AvaII BalI BanII BbvI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HgaI HhaI HinP1I HinfI
HphI ItaI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MvaI MvnI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TseI