Restriction map for
pFN21AE2022
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AviII AvrII BalI BbeI BbvCI
BcnI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BssHII Bst1107I
BstAPI BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV FauI
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 251 GGTACC G^GTACC
AccI 377 GTAGAC GT^[AC][GT]AC
AcsI 121 GAATTT [GA]^AATT[CT]
ApoI 121 GAATTT [GA]^AATT[CT]
Asp718I 251 GGTACC G^GTACC
BamHI 762 GGATCC G^GATCC
BbrPI 1701 CACGTG CAC^GTG
BbsI 1393 GAAGAC GAAGAC(2/6)
BcgI 608 GCAACCGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1509 TGATCA T^GATCA
BglI 1117 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1373 CTCCAG CTGGAG(16/14)
BpuAI 1393 GAAGAC GAAGAC(2/6)
BsaAI 1701 CACGTG [CT]AC^GT[GA]
BsaWI 1175 ACCGGA [AT]^CCGG[AT]
Bsp1407I 1082 TGTACA T^GTACA
BspMI 209 ACCTGC ACCTGC(4/8)
BsrGI 1082 TGTACA T^GTACA
BssSI 1576 CTCGTG CACGAG(-5/-1)
BstBI 264 TTCGAA TT^CGAA
BstEII 564 GGTAACC G^GT[ACGT]ACC
BstPI 564 GGTAACC G^GT[ACGT]ACC
Cac8I 1122 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 1665 GCGC GCG^C
DraI 933 TTTAAA TTT^AAA
DraII 1105 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 1115 TGGCCG [CT]^GGCC[GA]
EcoO109I 1105 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 564 GGTAACC G^GT[ACGT]ACC
EcoT14I 1502 CCTTGG C^C[AT][AT]GG
EcoT22I 455 ATGCAT ATGCA^T
FbaI 1509 TGATCA T^GATCA
HapII 1176 CCGG C^CGG
HgaI 1200 GACGC GACGC(5/10)
HhaI 1665 GCGC GCG^C
HinP1I 1665 GCGC G^CGC
HpaII 1176 CCGG C^CGG
KpnI 251 GGTACC GGTAC^C
MaeII 1702 ACGT A^CGT
MspI 1176 CCGG C^CGG
NdeI 653 CATATG CA^TATG
NsiI 455 ATGCAT ATGCA^T
NspI 1673 ACATGC [GA]CATG^[CT]
NspV 264 TTCGAA TT^CGAA
PacI 1531 TTAATTAA TTAAT^TAA
PmaCI 1701 CACGTG CAC^GTG
PmlI 1701 CACGTG CAC^GTG
PpuMI 1105 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 534 CAGCTG CAG^CTG
SexAI 568 ACCTGGT A^CC[AT]GGT
SfcI 1143 CTACAG C^T[GA][CT]AG
SfuI 264 TTCGAA TT^CGAA
SmlI 1440 CTTGAG C^T[CT][GA]AG
SspBI 1082 TGTACA T^GTACA
StyI 1502 CCTTGG C^C[AT][AT]GG
Tsp45I 976 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwNI 902 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
1146 CAGGCACTG
AocI 907 CCTGAGG CC^T[ACGT]AGG
1786 CCTAAGG
BanI 251 GGTACC G^G[CT][GA]CC
1096 GGCACC
BciVI 678 GGATAC GTATCC(6/5)
1234 GTATCC
BsmFI 1105 GGGAC GGGAC(10/14)
199 GTCCC
BstYI 273 AGATCC [GA]^GATC[CT]
762 GGATCC
Bsu36I 907 CCTGAGG CC^T[ACGT]AGG
1786 CCTAAGG
EarI 115 GAAGAG CTCTTC(1/4)
1424 GAAGAG
Eco81I 907 CCTGAGG CC^T[ACGT]AGG
1786 CCTAAGG
Ksp632I 115 GAAGAG CTCTTC(1/4)
1424 GAAGAG
MflI 273 AGATCC [GA]^GATC[CT]
762 GGATCC
MslI 653 CATATGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1674 CATGCCTGTG
MspA1I 534 CAGCTG C[AC]G^C[GT]G
560 CAGCGG
MwoI 1117 GCCGTGCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1126 GCGGCGAGTGC
NlaIII 219 CATG CATG^
1674 CATG
SacI 1251 GAGCTC GAGCT^C
1776 GAGCTC
SspI 61 AATATT AAT^ATT
1431 AATATT
XhoII 273 AGATCC [GA]^GATC[CT]
762 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbvI BfaI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MvaI NdeII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XcmI