Restriction map for
pFN21AE1878
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I CelII ClaI CpoI DraII
DrdI DsaI EagI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRV
EcoT14I EcoT22I FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI TaqI
Tth111I TthHB8I XbaI XcmI XhoI
XhoII
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 865 CGCG CG^CG
AflIII 14 ACGTGT A^C[GA][CT]GT
AhdI 349 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 937 GATCC GGATC(4/5)
AocI 141 CCTGAGG CC^T[ACGT]AGG
ApaI 260 GGGCCC GGGCC^C
Asp700I 332 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 349 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 855 CCCGGG C^[CT]CG[GA]G
BclI 713 TGATCA T^GATCA
BsaAI 13 TACGTG [CT]AC^GT[GA]
BsaI 700 GAGACC GGTCTC(1/5)
BseRI 1032 CTCCTC GAGGAG(10/8)
BsgI 321 CTGCAC GTGCAG(16/14)
BsoBI 855 CCCGGG C^[CT]CG[GA]G
BstUI 865 CGCG CG^CG
Bsu36I 141 CCTGAGG CC^T[ACGT]AGG
DraI 970 TTTAAA TTT^AAA
DraIII 583 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 824 CGGCCA [CT]^GGCC[GA]
Eam1105I 349 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 141 CCTGAGG CC^T[ACGT]AGG
EcoRI 469 GAATTC G^AATTC
FbaI 713 TGATCA T^GATCA
HaeII 847 AGCGCC [GA]GCGC^[CT]
HincII 432 GTCAAC GT[CT]^[GA]AC
HindII 432 GTCAAC GT[CT]^[GA]AC
MspA1I 615 CCGCTG C[AC]G^C[GT]G
MvnI 865 CGCG CG^CG
PflMI 809 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 364 GAGCTC GAGCT^C
SfcI 134 CTGTAG C^T[GA][CT]AG
SmaI 855 CCCGGG CCC^GGG
SmlI 727 CTCAAG C^T[CT][GA]AG
SspI 890 AATATT AAT^ATT
TspRI 1073 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 809 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 855 CCCGGG C^CCGGG
XmaI 855 CCCGGG C^CCGGG
XmnI 332 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 65 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
75 CAGAAGCTG
AspHI 272 GTGCTC G[AT]GC[AT]^C
364 GAGCTC
AvaII 231 GGTCC G^G[AT]CC
563 GGACC
BanII 260 GGGCCC G[GA]GC[CT]^C
364 GAGCTC
BbvI 70 GCTGC GCAGC(8/12)
80 GCTGC
BcnI 855 CCCGG CC^[GC]GG
856 CCGGG
BsiHKAI 272 GTGCTC G[AT]GC[AT]^C
364 GAGCTC
BsiYI 768 CCCGTGTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
809 CCATGCTGTGG
BslI 768 CCCGTGTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
809 CCATGCTGTGG
BsmAI 247 GAGAC GTCTC(1/5)
700 GAGAC
BsmFI 562 GGGAC GGGAC(10/14)
232 GTCCC
BsrFI 83 GCCGGT [GA]^CCGG[CT]
790 ACCGGC
BstF5I 254 GGATG GGATG(2/0)
1103 GGATG
CfoI 6 GCGC GCG^C
848 GCGC
Cfr10I 83 GCCGGT [GA]^CCGG[CT]
790 ACCGGC
DpnII 714 GATC ^GATC
937 GATC
FokI 254 GGATG GGATG(9/13)
1103 GGATG
HhaI 6 GCGC GCG^C
848 GCGC
HinP1I 6 GCGC G^CGC
848 GCGC
HphI 41 TCACC GGTGA(8/7)
473 TCACC
MboI 714 GATC ^GATC
937 GATC
NciI 855 CCCGG CC^[GC]GG
856 CCGGG
NdeII 714 GATC ^GATC
937 GATC
PleI 217 GACTC GAGTC(4/5)
304 GACTC
Sau3AI 714 GATC ^GATC
937 GATC
TfiI 498 GATTC G^A[AT]TC
514 GATTC
TseI 70 GCTGC G^C[AT]GC
80 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BanI BfaI BmyI BsaJI Bsp1286I
BsrI BssKI BstNI Cac8I Cfr13I
DdeI EarI EcoRII FauI Fnu4HI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeI MaeII MaeIII MboII
MnlI MseI MslI MspI MvaI
MwoI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI Tru9I Tsp45I Tsp509I