##################################
# NO CUT ENZYMES (  200 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BsaAI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      ClaI        CpoI        DpnII       
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MspA1I      
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   23 enzymes) #
###################################

BbsI          377  GTCTTC               GAAGAC(2/6)
BbvI          145  GCTGC                GCAGC(8/12)
BpuAI         377  GTCTTC               GAAGAC(2/6)
BsaBI         472  GATGTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI           31  GTGCAG               GTGCAG(16/14)
BsiYI         717  CCTGGCACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          717  CCTGGCACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrDI         644  CATTGC               GCAATG(2/0)
HaeIII        231  GGCC                 GG^CC
HapII          80  CCGG                 C^CGG
HindIII       334  AAGCTT               A^AGCTT
HpaII          80  CCGG                 C^CGG
HphI          737  TCACC                GGTGA(8/7)
MamI          472  GATGTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MnlI          691  CCTC                 CCTC(7/6)
MspI           80  CCGG                 C^CGG
MwoI          702  GCTCTACGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PleI           44  GAGTC                GAGTC(4/5)
ScaI           10  AGTACT               AGT^ACT
SfcI          287  CTATAG               C^T[GA][CT]AG
TaqI          207  TCGA                 T^CGA
TseI          145  GCTGC                G^C[AT]GC
TthHB8I       207  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AciI          117  CCGC                 CCGC(-3/-1)
               64  GCGG                
AluI          335  AGCT                 AG^CT
              524  AGCT                
AvaII         222  GGACC                G^G[AT]CC
              255  GGTCC               
BciVI         634  GGATAC               GTATCC(6/5)
              678  GTATCC              
BsmAI         325  GAGAC                GTCTC(1/5)
              440  GAGAC               
BsrI          723  ACTGG                ACTGG(1/-1)
              225  CCAGT               
DdeI           27  CTTAG                C^T[ACGT]AG
              103  CTGAG               
EcoRI          21  GAATTC               G^AATTC
               51  GAATTC              
Fnu4HI         64  GCGGC                GC^[ACGT]GC
              145  GCTGC               
ItaI           64  GCGGC                GC^[ACGT]GC
              145  GCTGC               
MaeIII          3  GTTAC                ^GT[ACGT]AC
              622  GTAAC               
MboII         378  TCTTC                GAAGA(8/7)
              672  TCTTC               
MseI          262  TTAA                 T^TAA
              394  TTAA                
MslI          478  CATCTCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              722  CACTGGGATG          
NlaIII        312  CATG                 CATG^
              541  CATG                
TfiI          317  GATTC                G^A[AT]TC
              696  GATTC               
Tru9I         262  TTAA                 T^TAA
              394  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AcsI        AfaI        ApoI        BsaJI       BssKI       
BstF5I      BstNI       Cfr13I      EcoRII      FokI        
HinfI       MvaI        RsaI        Sau96I      ScrFI       
SfaNI       Tsp509I     TspRI