Restriction map for
pFN21AE3970
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DpnII DraI DraII DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaI HpaII
KasI KpnI Ksp632I KspI MaeI
MaeII MamI MboI MfeI MflI
MluI MluNI MroI MscI MseI
MspI MunI NaeI NarI NciI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 56 CGCG CG^CG
AciI 348 CCGC CCGC(-3/-1)
AcyI 3 GGCGTC G[GA]^CG[CT]C
AfaI 453 GTAC GT^AC
BbsI 112 GAAGAC GAAGAC(2/6)
BpuAI 112 GAAGAC GAAGAC(2/6)
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsiYI 42 CCCTGAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 42 CCCTGAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 52 GAGACG CGTCTC(1/5)
BsmI 305 GCATTC GAATGC(1/-1)
Bsp1407I 452 TGTACA T^GTACA
BspMI 146 ACCTGC ACCTGC(4/8)
BsrGI 452 TGTACA T^GTACA
BsrI 429 ACTGG ACTGG(1/-1)
BstF5I 101 GGATG GGATG(2/0)
BstUI 56 CGCG CG^CG
BstXI 164 CCAAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 441 CCATGG C^C[GA][CT]GG
EcoT14I 441 CCATGG C^C[AT][AT]GG
FokI 101 GGATG GGATG(9/13)
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HphI 285 TCACC GGTGA(8/7)
MaeIII 87 GTCAC ^GT[ACGT]AC
MboII 112 GAAGA GAAGA(8/7)
MslI 267 CATTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 56 CGCG CG^CG
NcoI 441 CCATGG C^CATGG
NspI 65 ACATGC [GA]CATG^[CT]
RsaI 453 GTAC GT^AC
SfaNI 273 GATGC GCATC(5/9)
SmlI 220 CTCAAG C^T[CT][GA]AG
SspBI 452 TGTACA T^GTACA
StyI 441 CCATGG C^C[AT][AT]GG
Tsp45I 87 GTCAC ^GT[GC]AC
TspRI 363 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 62 CCAACATGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 244 GAATTT [GA]^AATT[CT]
330 AAATTC
ApoI 244 GAATTT [GA]^AATT[CT]
330 AAATTC
AvaII 39 GGACC G^G[AT]CC
138 GGACC
BanII 76 GAGCTC G[GA]GC[CT]^C
217 GAGCTC
BlpI 214 GCTGAGC GC^T[ACGT]AGC
318 GCTAAGC
BpmI 73 CTGGAG CTGGAG(16/14)
430 CTGGAG
Bpu1102I 214 GCTGAGC GC^T[ACGT]AGC
318 GCTAAGC
BsmAI 52 GAGAC GTCTC(1/5)
259 GAGAC
BsmFI 137 GGGAC GGGAC(10/14)
338 GTCCC
BssKI 188 CCAGG ^CC[ACGT]GG
314 CCTGG
BstNI 188 CCAGG CC^[AT]GG
314 CCTGG
Cac8I 29 GCGAGC GC[ACGT]^[ACGT]GC
78 GCTCGC
CelII 214 GCTGAGC GC^T[ACGT]AGC
318 GCTAAGC
Cfr13I 39 GGACC G^G[ACGT]CC
138 GGACC
EcoRII 188 CCAGG ^CC[AT]GG
314 CCTGG
HgaI 54 GACGC GACGC(5/10)
4 GCGTC
MspA1I 158 CAGCTG C[AC]G^C[GT]G
300 CAGCTG
MvaI 188 CCAGG CC^[AT]GG
314 CCTGG
MwoI 48 GCAGGAGACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
69 GCTGCTGGAGC
PvuII 158 CAGCTG CAG^CTG
300 CAGCTG
SacI 76 GAGCTC GAGCT^C
217 GAGCTC
Sau96I 39 GGACC G^G[ACGT]CC
138 GGACC
ScrFI 188 CCAGG CC^[ACGT]GG
314 CCTGG
TaqI 14 TCGA T^CGA
405 TCGA
Tsp509I 245 AATT ^AATT
331 AATT
TthHB8I 14 TCGA T^CGA
405 TCGA
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI AspHI BbvI BmyI BsaJI
BseRI BsiHKAI Bsp1286I DdeI Fnu4HI
ItaI MnlI NlaIII NlaIV TseI