Restriction map for
pFN21AB7891
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# NO CUT ENZYMES ( 90 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI Aor51HI ApoI
AscI AseI AsnI AviII AvrII
BalI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaWI BseAI
BsiWI Bsp1407I BspEI BspHI BspLU11I
BsrGI Bst1107I BstBI BstZ17I CelII
CpoI DrdI Eco47III EcoRI EcoRV
FbaI FseI FspI HgaI HindIII
HpaI MamI MfeI MluI MluNI
MroI MscI MslI MunI NcoI
NdeI NheI NotI NruI NspV
PacI PflMI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tsp509I Van91I XbaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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Acc65I 362 GGTACC G^GTACC
AcyI 257 GGCGCC G[GA]^CG[CT]C
AhdI 496 GACACGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1101 GTGCAC G^TGCAC
AlwNI 1369 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1535 GGGCCC GGGCC^C
ApaLI 1101 GTGCAC G^TGCAC
Asp700I 1050 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 362 GGTACC G^GTACC
AspEI 496 GACACGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1101 GTGCAC G[AT]GC[AT]^C
BamHI 911 GGATCC G^GATCC
BbeI 257 GGCGCC GGCGC^C
BbrPI 1033 CACGTG CAC^GTG
BfaI 184 CTAG C^TAG
BsaAI 1033 CACGTG [CT]AC^GT[GA]
BsaHI 257 GGCGCC G[GA]^CG[CT]C
BsaI 849 GGTCTC GGTCTC(1/5)
BsgI 1118 GTGCAG GTGCAG(16/14)
BsiEI 74 CGGCCG CG[GA][CT]^CG
BsiHKAI 1101 GTGCAC G[AT]GC[AT]^C
BsmBI 930 CGTCTC CGTCTC(1/5)
BsmI 1050 GAATGC GAATGC(1/-1)
BspDI 643 ATCGAT AT^CGAT
BsrBI 1204 GAGCGG CCGCTC(-3/-3)
BsrDI 593 GCAATG GCAATG(2/0)
BssHII 433 GCGCGC G^CGCGC
BssSI 498 CACGAG CACGAG(-5/-1)
ClaI 643 ATCGAT AT^CGAT
DraI 340 TTTAAA TTT^AAA
DraIII 871 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 74 CGGCCG C^GGCCG
Eam1105I 496 GACACGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 74 CGGCCG C^GGCCG
Eco52I 74 CGGCCG C^GGCCG
EcoNI 183 CCTAGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 562 ATGCAT ATGCA^T
Hin1I 257 GGCGCC G[GA]^CG[CT]C
HincII 208 GTCAAC GT[CT]^[GA]AC
HindII 208 GTCAAC GT[CT]^[GA]AC
KasI 257 GGCGCC G^GCGCC
KpnI 362 GGTACC GGTAC^C
KspI 292 CCGCGG CCGC^GG
MaeI 184 CTAG C^TAG
MaeII 1034 ACGT A^CGT
NaeI 76 GCCGGC GCC^GGC
NarI 257 GGCGCC GG^CGCC
NgoMIV 76 GCCGGC G^CCGGC
NsiI 562 ATGCAT ATGCA^T
PaeR7I 1163 CTCGAG C^TCGAG
PmaCI 1033 CACGTG CAC^GTG
PmlI 1033 CACGTG CAC^GTG
PshAI 413 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 292 CCGCGG CCGC^GG
ScaI 1181 AGTACT AGT^ACT
SexAI 1234 ACCTGGT A^CC[AT]GGT
SfoI 257 GGCGCC GGC^GCC
SmlI 1163 CTCGAG C^T[CT][GA]AG
SphI 1421 GCATGC GCATG^C
StuI 319 AGGCCT AGG^CCT
TfiI 659 GAATC G^A[AT]TC
TspRI 980 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 1655 CCACAGCTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1163 CTCGAG C^TCGAG
XmnI 1050 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
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AocI 322 CCTCAGG CC^T[ACGT]AGG
905 CCTCAGG
AspI 187 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1299 GACACAGTC
BanII 972 GAGCCC G[GA]GC[CT]^C
1535 GGGCCC
BspMI 443 ACCTGC ACCTGC(4/8)
1373 ACCTGC
BsrFI 76 GCCGGC [GA]^CCGG[CT]
954 ACCGGC
BsrI 854 CCAGT ACTGG(1/-1)
997 CCAGT
BstAPI 555 GCATCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1327 GCAGAGGGTGC
BstEII 503 GGTCACC G^GT[ACGT]ACC
1746 GGTCACC
BstF5I 565 CATCC GGATG(2/0)
680 CATCC
BstPI 503 GGTCACC G^GT[ACGT]ACC
1746 GGTCACC
BstXI 1085 CCAAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1131 CCAGATCCTTGG
BstYI 911 GGATCC [GA]^GATC[CT]
1133 AGATCC
Bsu36I 322 CCTCAGG CC^T[ACGT]AGG
905 CCTCAGG
Cfr10I 76 GCCGGC [GA]^CCGG[CT]
954 ACCGGC
DsaI 292 CCGCGG C^C[GA][CT]GG
1413 CCACGG
EaeI 74 CGGCCG [CT]^GGCC[GA]
1436 CGGCCA
EarI 1269 GAAGAG CTCTTC(1/4)
1275 GAAGAG
Eco81I 322 CCTCAGG CC^T[ACGT]AGG
905 CCTCAGG
EcoO65I 503 GGTCACC G^GT[ACGT]ACC
1746 GGTCACC
FokI 565 CATCC GGATG(9/13)
680 CATCC
Ksp632I 1269 GAAGAG CTCTTC(1/4)
1275 GAAGAG
MflI 911 GGATCC [GA]^GATC[CT]
1133 AGATCC
MseI 341 TTAA T^TAA
1127 TTAA
NspI 560 ACATGC [GA]CATG^[CT]
1421 GCATGC
PflFI 187 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1299 GACACAGTC
PpuMI 179 GGGACCT [GA]G^G[AT]CC[CT]
345 AGGACCC
SfaNI 555 GCATC GCATC(5/9)
564 GCATC
SmaI 1244 CCCGGG CCC^GGG
1532 CCCGGG
Tru9I 341 TTAA T^TAA
1127 TTAA
Tth111I 187 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1299 GACACAGTC
XhoII 911 GGATCC [GA]^GATC[CT]
1133 AGATCC
XmaCI 1244 CCCGGG C^CCGGG
1532 CCCGGG
XmaI 1244 CCCGGG C^CCGGG
1532 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI AvaII BanI BbvI BcnI
BglI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
StyI TaqI TseI Tsp45I TthHB8I