Restriction map for
pFN21AB6282
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbrPI
BcgI BlnI BsaI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrFI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV FseI FspI HgaI
HpaI KpnI KspI MfeI MluI
MroI MslI MspA1I MunI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SfuI SgrAI SpeI
Sse8387I SwaI Tth111I Van91I XcmI
XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 2114 GTCTAC GT^[AC][GT]AC
AflII 1534 CTTAAG C^TTAAG
AocI 867 CCTGAGG CC^T[ACGT]AGG
AspHI 216 GAGCTC G[AT]GC[AT]^C
AvaI 111 CCCGGG C^[CT]CG[GA]G
AvaII 108 GGTCC G^G[AT]CC
BalI 446 TGGCCA TGG^CCA
BamHI 1710 GGATCC G^GATCC
BbvCI 607 GCTGAGG CCTCAGC(-5/-2)
BciVI 1878 GTATCC GTATCC(6/5)
BclI 333 TGATCA T^GATCA
BfrI 1534 CTTAAG C^TTAAG
BglI 188 GCCCTCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 729 AGATCT A^GATCT
BlpI 79 GCTGAGC GC^T[ACGT]AGC
BpmI 140 CTGGAG CTGGAG(16/14)
Bpu1102I 79 GCTGAGC GC^T[ACGT]AGC
BsaAI 886 TACGTA [CT]AC^GT[GA]
BsaBI 1522 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 2198 CTGCAC GTGCAG(16/14)
BsiHKAI 216 GAGCTC G[AT]GC[AT]^C
BsoBI 111 CCCGGG C^[CT]CG[GA]G
Bsp1407I 480 TGTACA T^GTACA
BspMI 156 GCAGGT ACCTGC(4/8)
BsrBI 858 CCGCTC CCGCTC(-3/-3)
BsrGI 480 TGTACA T^GTACA
BsrI 793 CCAGT ACTGG(1/-1)
BssSI 191 CTCGTG CACGAG(-5/-1)
BstXI 2119 CCAGAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 867 CCTGAGG CC^T[ACGT]AGG
CelII 79 GCTGAGC GC^T[ACGT]AGC
DsaI 1567 CCATGG C^C[GA][CT]GG
Eco81I 867 CCTGAGG CC^T[ACGT]AGG
FbaI 333 TGATCA T^GATCA
HincII 1719 GTCAAC GT[CT]^[GA]AC
HindII 1719 GTCAAC GT[CT]^[GA]AC
MamI 1522 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 446 TGGCCA TGG^CCA
MscI 446 TGGCCA TGG^CCA
NcoI 1567 CCATGG C^CATGG
SacI 216 GAGCTC GAGCT^C
SexAI 2099 ACCAGGT A^CC[AT]GGT
SfiI 187 GGCCCTCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 111 CCCGGG CCC^GGG
SmlI 1534 CTTAAG C^T[CT][GA]AG
SnaBI 886 TACGTA TAC^GTA
SphI 1053 GCATGC GCATG^C
SspBI 480 TGTACA T^GTACA
StuI 1947 AGGCCT AGG^CCT
Tsp45I 1340 GTGAC ^GT[GC]AC
XbaI 2212 TCTAGA T^CTAGA
XmaCI 111 CCCGGG C^CCGGG
XmaI 111 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 61 GGCGCC G[GA]^CG[CT]C
90 GGCGCC
ApaI 186 GGGCCC GGGCC^C
452 GGGCCC
Asp700I 1435 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2242 GAAATAATTC
BanI 61 GGCGCC G^G[CT][GA]CC
90 GGCGCC
BbeI 61 GGCGCC GGCGC^C
90 GGCGCC
BbsI 1235 GAAGAC GAAGAC(2/6)
345 GTCTTC
BpuAI 1235 GAAGAC GAAGAC(2/6)
345 GTCTTC
BsaHI 61 GGCGCC G[GA]^CG[CT]C
90 GGCGCC
BsaWI 105 TCCGGT [AT]^CCGG[AT]
176 ACCGGA
BsmAI 722 GAGAC GTCTC(1/5)
1153 GAGAC
BsmFI 165 GGGAC GGGAC(10/14)
109 GTCCC
BsrDI 1750 CATTGC GCAATG(2/0)
1425 GCAATG
BssHII 33 GCGCGC G^CGCGC
152 GCGCGC
EaeI 195 TGGCCG [CT]^GGCC[GA]
446 TGGCCA
EcoRI 1699 GAATTC G^AATTC
2221 GAATTC
EcoT14I 1335 CCAAGG C^C[AT][AT]GG
1567 CCATGG
EcoT22I 1051 ATGCAT ATGCA^T
1476 ATGCAT
HaeII 61 GGCGCC [GA]GCGC^[CT]
90 GGCGCC
Hin1I 61 GGCGCC G[GA]^CG[CT]C
90 GGCGCC
KasI 61 GGCGCC G^GCGCC
90 GGCGCC
NarI 61 GGCGCC GG^CGCC
90 GGCGCC
NsiI 1051 ATGCAT ATGCA^T
1476 ATGCAT
NspI 1053 GCATGC [GA]CATG^[CT]
2110 GCATGT
SapI 127 GAAGAGC GCTCTTC(1/4)
1956 GCTCTTC
SfoI 61 GGCGCC GGC^GCC
90 GGCGCC
SspI 1405 AATATT AAT^ATT
1966 AATATT
StyI 1335 CCAAGG C^C[AT][AT]GG
1567 CCATGG
TaqI 1221 TCGA T^CGA
2237 TCGA
TthHB8I 1221 TCGA T^CGA
2237 TCGA
XmnI 1435 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2242 GAAATAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI BanII BbvI BcnI
BfaI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI Tru9I
TseI Tsp509I TspRI XhoII