Restriction map for
pFN21AB7578
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvrII BamHI BbrPI BbsI
BbvCI BcgI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsiHKAI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspHI BspLU11I
BsrDI BsrGI BsrI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII CpoI DraI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI KspI MaeIII
MamI MfeI MseI MslI MunI
NcoI NdeI NheI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccIII 217 TCCGGA T^CCGGA
AfaI 540 GTAC GT^AC
AflIII 710 ACGCGT A^C[GA][CT]GT
BalI 569 TGGCCA TGG^CCA
BciVI 157 GGATAC GTATCC(6/5)
BpmI 508 CTGGAG CTGGAG(16/14)
BseAI 217 TCCGGA T^CCGGA
BseRI 652 GAGGAG GAGGAG(10/8)
BsgI 760 CTGCAC GTGCAG(16/14)
BspDI 61 ATCGAT AT^CGAT
BspEI 217 TCCGGA T^CCGGA
BspMI 791 ACCTGC ACCTGC(4/8)
BssSI 40 CACGAG CACGAG(-5/-1)
BstAPI 786 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 123 CCAGGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 68 AGATCC [GA]^GATC[CT]
ClaI 61 ATCGAT AT^CGAT
DdeI 73 CTGAG C^T[ACGT]AG
DraII 307 GGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 627 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 592 GACGCACAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 775 CTCTTC CTCTTC(1/4)
EcoO109I 307 GGGCCCT [GA]G^G[ACGT]CC[CT]
HinfI 344 GACTC G^A[ACGT]TC
HphI 347 TCACC GGTGA(8/7)
Ksp632I 775 CTCTTC CTCTTC(1/4)
MflI 68 AGATCC [GA]^GATC[CT]
MluI 710 ACGCGT A^CGCGT
MluNI 569 TGGCCA TGG^CCA
MroI 217 TCCGGA T^CCGGA
MscI 569 TGGCCA TGG^CCA
PleI 344 GACTC GAGTC(4/5)
RsaI 540 GTAC GT^AC
SapI 774 GCTCTTC GCTCTTC(1/4)
SfiI 722 GGCCGCTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI 62 TCGA T^CGA
TthHB8I 62 TCGA T^CGA
XhoII 68 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AluI 52 AGCT AG^CT
214 AGCT
AlwI 69 GATCC GGATC(4/5)
240 GATCC
ApaI 225 GGGCCC GGGCC^C
307 GGGCCC
AviII 252 TGCGCA TGC^GCA
581 TGCGCA
BfaI 103 CTAG C^TAG
337 CTAG
BsaWI 217 TCCGGA [AT]^CCGG[AT]
452 ACCGGA
BstF5I 11 GGATG GGATG(2/0)
359 GGATG
DsaI 162 CCACGG C^C[GA][CT]GG
718 CCACGG
EcoT14I 383 CCAAGG C^C[AT][AT]GG
572 CCAAGG
FokI 11 GGATG GGATG(9/13)
359 GGATG
FspI 252 TGCGCA TGC^GCA
581 TGCGCA
MaeI 103 CTAG C^TAG
337 CTAG
MaeII 484 ACGT A^CGT
607 ACGT
MboII 471 GAAGA GAAGA(8/7)
776 TCTTC
NaeI 187 GCCGGC GCC^GGC
683 GCCGGC
NgoMIV 187 GCCGGC G^CCGGC
683 GCCGGC
NlaIII 130 CATG CATG^
585 CATG
NotI 368 GCGGCCGC GC^GGCCGC
729 GCGGCCGC
StyI 383 CCAAGG C^C[AT][AT]GG
572 CCAAGG
TspRI 299 CAGTG CA[GC]TG[ACGT][ACGT]^
827 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcyI AlwNI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BglI BmyI BsaHI BsaJI
BsiEI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DpnII EaeI EagI EclXI Eco52I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII ItaI KasI MboI MnlI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIV PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI StuI TseI