Restriction map for
pFN21AE6385
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# NO CUT ENZYMES ( 120 enzymes) #
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Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI AlwI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsiWI BsmI BspDI BspHI
BspLU11I BsrBI BsrFI BssHII Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoT14I EcoT22I FbaI FseI FspI
HindIII HinfI HpaI KasI KpnI
KspI MamI MluI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I RcaI RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI SspI StuI
StyI SwaI TfiI Tth111I XbaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccII 764 CGCG CG^CG
AccIII 288 TCCGGA T^CCGGA
Alw44I 257 GTGCAC G^TGCAC
ApaLI 257 GTGCAC G^TGCAC
Asp700I 303 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 257 GTGCAC G[AT]GC[AT]^C
BalI 914 TGGCCA TGG^CCA
BanII 714 GGGCTC G[GA]GC[CT]^C
BglII 305 AGATCT A^GATCT
BsaWI 288 TCCGGA [AT]^CCGG[AT]
BseAI 288 TCCGGA T^CCGGA
BsgI 190 GTGCAG GTGCAG(16/14)
BsiEI 803 CGATCG CG[GA][CT]^CG
BsiHKAI 257 GTGCAC G[AT]GC[AT]^C
Bsp1407I 1004 TGTACA T^GTACA
BspEI 288 TCCGGA T^CCGGA
BspMI 474 ACCTGC ACCTGC(4/8)
BsrGI 1004 TGTACA T^GTACA
BstEII 234 GGTCACC G^GT[ACGT]ACC
BstPI 234 GGTCACC G^GT[ACGT]ACC
BstUI 764 CGCG CG^CG
BstXI 527 CCATTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 305 AGATCT [GA]^GATC[CT]
DraII 217 GGGACCC [GA]G^G[ACGT]CC[CT]
EarI 925 CTCTTC CTCTTC(1/4)
EcoO109I 217 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 234 GGTCACC G^GT[ACGT]ACC
EcoRV 136 GATATC GAT^ATC
HincII 439 GTCAAC GT[CT]^[GA]AC
HindII 439 GTCAAC GT[CT]^[GA]AC
Ksp632I 925 CTCTTC CTCTTC(1/4)
MfeI 353 CAATTG C^AATTG
MflI 305 AGATCT [GA]^GATC[CT]
MluNI 914 TGGCCA TGG^CCA
MroI 288 TCCGGA T^CCGGA
MscI 914 TGGCCA TGG^CCA
MseI 542 TTAA T^TAA
MunI 353 CAATTG C^AATTG
MvnI 764 CGCG CG^CG
NheI 46 GCTAGC G^CTAGC
PaeR7I 54 CTCGAG C^TCGAG
PflMI 381 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 217 GGGACCC [GA]G^G[AT]CC[CT]
PstI 838 CTGCAG CTGCA^G
PvuI 803 CGATCG CGAT^CG
PvuII 193 CAGCTG CAG^CTG
SbfI 837 CCTGCAGG CCTGCA^GG
SmaI 228 CCCGGG CCC^GGG
SmlI 54 CTCGAG C^T[CT][GA]AG
Sse8387I 837 CCTGCAGG CCTGCA^GG
SspBI 1004 TGTACA T^GTACA
Tru9I 542 TTAA T^TAA
Van91I 381 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 54 CTCGAG C^TCGAG
XhoII 305 AGATCT [GA]^GATC[CT]
XmaCI 228 CCCGGG C^CCGGG
XmaI 228 CCCGGG C^CCGGG
XmnI 303 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 102 GACGTC GACGT^C
173 GACGTC
AfaI 660 GTAC GT^AC
1005 GTAC
AvaI 54 CTCGAG C^[CT]CG[GA]G
228 CCCGGG
BciVI 6 GGATAC GTATCC(6/5)
496 GGATAC
BfaI 38 CTAG C^TAG
47 CTAG
BmyI 257 GTGCAC G[AGT]GC[ACT]^C
714 GGGCTC
BseRI 717 CTCCTC GAGGAG(10/8)
859 CTCCTC
BsmFI 217 GGGAC GGGAC(10/14)
346 GGGAC
BsoBI 54 CTCGAG C^[CT]CG[GA]G
228 CCCGGG
Bsp1286I 257 GTGCAC G[AGT]GC[ACT]^C
714 GGGCTC
DdeI 167 CTGAG C^T[ACGT]AG
178 CTCAG
EaeI 114 TGGCCG [CT]^GGCC[GA]
914 TGGCCA
HgaI 348 GACGC GACGC(5/10)
762 GACGC
MaeI 38 CTAG C^TAG
47 CTAG
MaeII 103 ACGT A^CGT
174 ACGT
MaeIII 163 GTCAC ^GT[ACGT]AC
235 GTCAC
MspA1I 193 CAGCTG C[AC]G^C[GT]G
910 CCGCTG
RsaI 660 GTAC GT^AC
1005 GTAC
TaqI 55 TCGA T^CGA
143 TCGA
Tsp45I 163 GTCAC ^GT[GC]AC
235 GTCAC
Tsp509I 354 AATT ^AATT
959 AATT
TthHB8I 55 TCGA T^CGA
143 TCGA
XcmI 377 CCAACCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
779 CCATGCCCATCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcyI AluI AlwNI AvaII
BbsI BbvI BcnI BpmI BpuAI
BsaHI BsaJI BsiYI BslI BsmAI
BsmBI BsrDI BsrI BssKI BssSI
BstF5I BstNI Cac8I CfoI Cfr13I
DpnII EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII HphI ItaI MboI
MboII MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI TspRI