Restriction map for
pFN21AE1988
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MluI MroI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PacI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Van91I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 1132 GTAGAC GT^[AC][GT]AC
AccII 1289 CGCG CG^CG
AcyI 1135 GACGCC G[GA]^CG[CT]C
AlwNI 616 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 540 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 64 GTGCTC G[AT]GC[AT]^C
BanI 1653 GGCACC G^G[CT][GA]CC
BbsI 289 GAAGAC GAAGAC(2/6)
BpuAI 289 GAAGAC GAAGAC(2/6)
BsaHI 1135 GACGCC G[GA]^CG[CT]C
BsaI 500 GGTCTC GGTCTC(1/5)
BsiHKAI 64 GTGCTC G[AT]GC[AT]^C
BsmI 1242 GAATGC GAATGC(1/-1)
BsrBI 13 GAGCGG CCGCTC(-3/-3)
BsrDI 435 CATTGC GCAATG(2/0)
BstAPI 1237 GCAAAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1289 CGCG CG^CG
DsaI 5 CCACGG C^C[GA][CT]GG
EcoRI 1276 GAATTC G^AATTC
EcoT14I 1678 CCAAGG C^C[AT][AT]GG
EcoT22I 358 ATGCAT ATGCA^T
Hin1I 1135 GACGCC G[GA]^CG[CT]C
MaeII 752 ACGT A^CGT
MaeIII 793 GTCAC ^GT[ACGT]AC
MfeI 518 CAATTG C^AATTG
MunI 518 CAATTG C^AATTG
MvnI 1289 CGCG CG^CG
NdeI 690 CATATG CA^TATG
NsiI 358 ATGCAT ATGCA^T
PaeR7I 503 CTCGAG C^TCGAG
PstI 768 CTGCAG CTGCA^G
SfcI 768 CTGCAG C^T[GA][CT]AG
SmlI 503 CTCGAG C^T[CT][GA]AG
StyI 1678 CCAAGG C^C[AT][AT]GG
Tsp45I 793 GTCAC ^GT[GC]AC
XhoI 503 CTCGAG C^TCGAG
XmnI 540 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwI 984 GATCC GGATC(4/5)
585 GGATC
AspI 757 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
787 GACTCGGTC
AvaI 260 CCCGAG C^[CT]CG[GA]G
503 CTCGAG
BanII 20 GAGCCC G[GA]GC[CT]^C
1203 GGGCTC
BglII 465 AGATCT A^GATCT
507 AGATCT
BsoBI 260 CCCGAG C^[CT]CG[GA]G
503 CTCGAG
BstYI 465 AGATCT [GA]^GATC[CT]
507 AGATCT
HphI 935 TCACC GGTGA(8/7)
1451 TCACC
MflI 465 AGATCT [GA]^GATC[CT]
507 AGATCT
MspA1I 559 CAGCTG C[AC]G^C[GT]G
674 CAGCTG
NspI 356 ACATGC [GA]CATG^[CT]
741 ACATGC
PflFI 757 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
787 GACTCGGTC
PvuII 559 CAGCTG CAG^CTG
674 CAGCTG
StuI 312 AGGCCT AGG^CCT
1681 AGGCCT
Tth111I 757 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
787 GACTCGGTC
XhoII 465 AGATCT [GA]^GATC[CT]
507 AGATCT
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BalI BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
MaeI MboI MboII MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I