Restriction map for
pFN21AE2298
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BclI
BcnI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BseAI
BsgI BsiEI BsiHKAI BsiWI BsoBI
Bsp1407I BspDI BspEI BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcyI 1213 GGCGTC G[GA]^CG[CT]C
AfaI 249 GTAC GT^AC
AflII 187 CTTAAG C^TTAAG
AflIII 251 ACATGT A^C[GA][CT]GT
AspI 139 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 691 GCTGAGG CCTCAGC(-5/-2)
BcgI 453 GCAAAGGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1036 GGATAC GTATCC(6/5)
BfrI 187 CTTAAG C^TTAAG
BglII 333 AGATCT A^GATCT
BsaHI 1213 GGCGTC G[GA]^CG[CT]C
BsaWI 485 ACCGGA [AT]^CCGG[AT]
BsmBI 1251 CGTCTC CGTCTC(1/5)
BsmI 1051 GAATGC GAATGC(1/-1)
BspHI 71 TCATGA T^CATGA
BspLU11I 251 ACATGT A^CATGT
BsrBI 979 CCGCTC CCGCTC(-3/-3)
BsrFI 348 GCCGGT [GA]^CCGG[CT]
BstYI 333 AGATCT [GA]^GATC[CT]
CfoI 26 GCGC GCG^C
Cfr10I 348 GCCGGT [GA]^CCGG[CT]
DraI 1231 TTTAAA TTT^AAA
DraII 973 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 1207 GACAAAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1056 CCGTGG C^C[GA][CT]GG
EaeI 1247 TGGCCG [CT]^GGCC[GA]
EcoO109I 973 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1137 GAATTC G^AATTC
FauI 978 CCCGC CCCGC(4/6)
HgaI 1214 GCGTC GACGC(5/10)
HhaI 26 GCGC GCG^C
Hin1I 1213 GGCGTC G[GA]^CG[CT]C
HinP1I 26 GCGC G^CGC
HincII 145 GTCGAC GT[CT]^[GA]AC
HindII 145 GTCGAC GT[CT]^[GA]AC
HindIII 1148 AAGCTT A^AGCTT
MaeII 624 ACGT A^CGT
MflI 333 AGATCT [GA]^GATC[CT]
MslI 444 CACACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 655 CAGCTG C[AC]G^C[GT]G
PflFI 139 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 973 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 655 CAGCTG CAG^CTG
RcaI 71 TCATGA T^CATGA
RsaI 249 GTAC GT^AC
SalI 145 GTCGAC G^TCGAC
SfcI 1266 CTGTAG C^T[GA][CT]AG
SphI 1104 GCATGC GCATG^C
StuI 1118 AGGCCT AGG^CCT
Tth111I 139 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 647 CCACCCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 333 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 145 GTCGAC GT^[AC][GT]AC
481 GTAGAC
AciI 979 CCGC CCGC(-3/-1)
1030 CCGC
AcsI 271 AAATTT [GA]^AATT[CT]
1137 GAATTC
AlwI 459 GGATC GGATC(4/5)
1019 GGATC
ApoI 271 AAATTT [GA]^AATT[CT]
1137 GAATTC
BbvI 43 GCAGC GCAGC(8/12)
62 GCAGC
BseRI 89 CTCCTC GAGGAG(10/8)
382 CTCCTC
BsmFI 1010 GGGAC GGGAC(10/14)
108 GTCCC
EcoT14I 230 CCAAGG C^C[AT][AT]GG
1121 CCTTGG
Fnu4HI 43 GCAGC GC^[ACGT]GC
62 GCAGC
HapII 349 CCGG C^CGG
486 CCGG
HpaII 349 CCGG C^CGG
486 CCGG
ItaI 43 GCAGC GC^[ACGT]GC
62 GCAGC
MspI 349 CCGG C^CGG
486 CCGG
PflMI 651 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1116 CCAGGCCTTGG
PleI 925 GACTC GAGTC(4/5)
1012 GACTC
SmlI 187 CTTAAG C^T[CT][GA]AG
1132 CTCAAG
StyI 230 CCAAGG C^C[AT][AT]GG
1121 CCTTGG
TaqI 146 TCGA T^CGA
989 TCGA
TfiI 223 GATTC G^A[AT]TC
938 GAATC
TseI 43 GCAGC G^C[AT]GC
62 GCAGC
TthHB8I 146 TCGA T^CGA
989 TCGA
Van91I 651 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1116 CCAGGCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AluI AvaII BanII BfaI BmyI
BpmI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII FokI HaeIII HinfI HphI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV NspI Sau3AI Sau96I
ScrFI SfaNI Tru9I Tsp45I Tsp509I
TspRI