Restriction map for
pFN21AE3181
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwNI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BciVI BcnI
BfrI BglI BglII BlnI BpmI
BsaBI BsaWI BseAI BsgI BsiWI
BsmBI BsmI BspDI BspEI BspLU11I
BspMI BsrBI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CfoI ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
FauI FseI FspI HaeII HhaI
HinP1I HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NarI
NciI NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tsp45I Tth111I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccI 312 GTCGAC GT^[AC][GT]AC
AciI 932 GCGG CCGC(-3/-1)
AcyI 1066 GACGCC G[GA]^CG[CT]C
Alw44I 1193 GTGCAC G^TGCAC
AlwI 1143 GATCC GGATC(4/5)
ApaI 248 GGGCCC GGGCC^C
ApaLI 1193 GTGCAC G^TGCAC
AspHI 1193 GTGCAC G[AT]GC[AT]^C
AvaI 245 CTCGGG C^[CT]CG[GA]G
BanII 248 GGGCCC G[GA]GC[CT]^C
BcgI 835 CGACCTCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 12 TGATCA T^GATCA
BlpI 670 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 670 GCTCAGC GC^T[ACGT]AGC
BsaAI 653 TACGTG [CT]AC^GT[GA]
BsaHI 1066 GACGCC G[GA]^CG[CT]C
BsaI 80 GGTCTC GGTCTC(1/5)
BsaJI 913 CCATGG C^C[ACGT][ACGT]GG
BseRI 185 CTCCTC GAGGAG(10/8)
BsiEI 310 CGGTCG CG[GA][CT]^CG
BsiHKAI 1193 GTGCAC G[AT]GC[AT]^C
BsmAI 81 GTCTC GTCTC(1/5)
BsoBI 245 CTCGGG C^[CT]CG[GA]G
Bsp1407I 939 TGTACA T^GTACA
BspHI 276 TCATGA T^CATGA
BsrDI 581 GCAATG GCAATG(2/0)
BsrGI 939 TGTACA T^GTACA
BstXI 936 CCATGTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1142 AGATCC [GA]^GATC[CT]
CelII 670 GCTCAGC GC^T[ACGT]AGC
DraI 130 TTTAAA TTT^AAA
DraII 1242 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 913 CCATGG C^C[GA][CT]GG
EarI 882 GAAGAG CTCTTC(1/4)
EcoO109I 1242 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 913 CCATGG C^C[AT][AT]GG
EcoT22I 571 ATGCAT ATGCA^T
FbaI 12 TGATCA T^GATCA
HgaI 1066 GACGC GACGC(5/10)
Hin1I 1066 GACGCC G[GA]^CG[CT]C
HincII 312 GTCGAC GT[CT]^[GA]AC
HindII 312 GTCGAC GT[CT]^[GA]AC
HindIII 806 AAGCTT A^AGCTT
Ksp632I 882 GAAGAG CTCTTC(1/4)
MflI 1142 AGATCC [GA]^GATC[CT]
MwoI 193 GCTGCAAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 1069 GCCGGC GCC^GGC
NcoI 913 CCATGG C^CATGG
NdeI 423 CATATG CA^TATG
NgoMIV 1069 GCCGGC G^CCGGC
NsiI 571 ATGCAT ATGCA^T
NspI 1104 GCATGT [GA]CATG^[CT]
PpuMI 1242 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 276 TCATGA T^CATGA
SalI 312 GTCGAC G^TCGAC
SspBI 939 TGTACA T^GTACA
StuI 771 AGGCCT AGG^CCT
StyI 913 CCATGG C^C[AT][AT]GG
TaqI 313 TCGA T^CGA
TthHB8I 313 TCGA T^CGA
XbaI 1248 TCTAGA T^CTAGA
XhoII 1142 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AluI 807 AGCT AG^CT
1050 AGCT
AocI 139 CCTAAGG CC^T[ACGT]AGG
187 CCTCAGG
BbsI 519 GAAGAC GAAGAC(2/6)
595 GAAGAC
BpuAI 519 GAAGAC GAAGAC(2/6)
595 GAAGAC
BsiYI 252 CCAGACTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
577 CCACGCAATGG
BslI 252 CCAGACTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
577 CCACGCAATGG
BsrFI 308 GCCGGT [GA]^CCGG[CT]
1069 GCCGGC
BsrI 956 ACTGG ACTGG(1/-1)
40 CCAGT
BssKI 355 CCTGG ^CC[ACGT]GG
607 CCTGG
BstNI 355 CCTGG CC^[AT]GG
607 CCTGG
Bsu36I 139 CCTAAGG CC^T[ACGT]AGG
187 CCTCAGG
Cac8I 412 GCCTGC GC[ACGT]^[ACGT]GC
1069 GCCGGC
Cfr10I 308 GCCGGT [GA]^CCGG[CT]
1069 GCCGGC
EaeI 306 TGGCCG [CT]^GGCC[GA]
933 CGGCCA
Eco81I 139 CCTAAGG CC^T[ACGT]AGG
187 CCTCAGG
EcoRII 355 CCTGG ^CC[AT]GG
607 CCTGG
HapII 309 CCGG C^CGG
1070 CCGG
HpaII 309 CCGG C^CGG
1070 CCGG
MaeIII 340 GTAAC ^GT[ACGT]AC
711 GTTAC
MspI 309 CCGG C^CGG
1070 CCGG
MvaI 355 CCTGG CC^[AT]GG
607 CCTGG
PflMI 252 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
577 CCACGCAATGG
PleI 475 GACTC GAGTC(4/5)
598 GACTC
ScrFI 355 CCTGG CC^[ACGT]GG
607 CCTGG
SfaNI 570 GATGC GCATC(5/9)
573 GCATC
TfiI 419 GAATC G^A[AT]TC
435 GAATC
TspRI 165 CACTG CA[GC]TG[ACGT][ACGT]^
841 CAGTG
Van91I 252 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
577 CCACGCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AfaI AvaII BbvI BfaI BmyI
BsmFI Bsp1286I BstF5I Cfr13I DdeI
DpnII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeII MboI
MboII MnlI MseI MslI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I SfcI Tru9I TseI Tsp509I