Restriction map for
pFN21AE3161
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AflIII AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AvaII AviII AvrII BalI BbeI
BbrPI BbvCI BcnI BfrI BglI
BglII BlnI BpmI BsaAI BsaBI
BsaHI BsaI BseAI BsgI BsiWI
BsmBI BsmFI BsoBI Bsp1407I BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Cfr13I CpoI
DraI DraII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HaeIII HgaI Hin1I HincII HindII
HindIII HpaI KasI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 949 GGTACC G^GTACC
AgeI 56 ACCGGT A^CCGGT
AocI 111 CCTTAGG CC^T[ACGT]AGG
Asp718I 949 GGTACC G^GTACC
AspHI 540 GAGCTC G[AT]GC[AT]^C
BamHI 822 GGATCC G^GATCC
BanI 949 GGTACC G^G[CT][GA]CC
BanII 540 GAGCTC G[GA]GC[CT]^C
BbsI 627 GAAGAC GAAGAC(2/6)
BcgI 17 CGAGCCTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 960 TGATCA T^GATCA
BfaI 1040 CTAG C^TAG
BlpI 798 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 798 GCTGAGC GC^T[ACGT]AGC
BpuAI 627 GAAGAC GAAGAC(2/6)
BsaWI 56 ACCGGT [AT]^CCGG[AT]
BsiEI 58 CGGTCG CG[GA][CT]^CG
BsiHKAI 540 GAGCTC G[AT]GC[AT]^C
BsiYI 50 CCCAGAACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 50 CCCAGAACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 101 GAGAC GTCTC(1/5)
BspDI 619 ATCGAT AT^CGAT
BspMI 391 ACCTGC ACCTGC(4/8)
BsrFI 56 ACCGGT [GA]^CCGG[CT]
BssSI 881 CTCGTG CACGAG(-5/-1)
BstAPI 418 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 822 GGATCC [GA]^GATC[CT]
Bsu36I 111 CCTTAGG CC^T[ACGT]AGG
CelII 798 GCTGAGC GC^T[ACGT]AGC
CfoI 27 GCGC GCG^C
Cfr10I 56 ACCGGT [GA]^CCGG[CT]
ClaI 619 ATCGAT AT^CGAT
DraIII 659 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 658 CCACGG C^C[GA][CT]GG
Eco81I 111 CCTTAGG CC^T[ACGT]AGG
FbaI 960 TGATCA T^GATCA
HapII 57 CCGG C^CGG
HhaI 27 GCGC GCG^C
HinP1I 27 GCGC G^CGC
HpaII 57 CCGG C^CGG
KpnI 949 GGTACC GGTAC^C
MaeI 1040 CTAG C^TAG
MflI 822 GGATCC [GA]^GATC[CT]
MspA1I 172 CAGCTG C[AC]G^C[GT]G
MspI 57 CCGG C^CGG
NlaIII 772 CATG CATG^
PinAI 56 ACCGGT A^CCGGT
PstI 476 CTGCAG CTGCA^G
PvuII 172 CAGCTG CAG^CTG
SacI 540 GAGCTC GAGCT^C
SfcI 476 CTGCAG C^T[GA][CT]AG
SpeI 1039 ACTAGT A^CTAGT
Tsp45I 7 GTGAC ^GT[GC]AC
XhoII 822 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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AccI 718 GTAGAC GT^[AC][GT]AC
1043 GTAGAC
AciI 4 GCGG CCGC(-3/-1)
385 GCGG
AcsI 846 AAATTT [GA]^AATT[CT]
923 GAATTT
AlwI 823 GATCC GGATC(4/5)
822 GGATC
ApoI 846 AAATTT [GA]^AATT[CT]
923 GAATTT
BmyI 33 GTGCCC G[AGT]GC[ACT]^C
540 GAGCTC
BseRI 314 CTCCTC GAGGAG(10/8)
317 CTCCTC
Bsp1286I 33 GTGCCC G[AGT]GC[ACT]^C
540 GAGCTC
Cac8I 16 GCGAGC GC[ACGT]^[ACGT]GC
673 GCACGC
EarI 98 GAAGAG CTCTTC(1/4)
346 GAAGAG
Ksp632I 98 GAAGAG CTCTTC(1/4)
346 GAAGAG
MaeIII 7 GTGAC ^GT[ACGT]AC
840 GTTAC
MwoI 20 GCCTGTTGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
418 GCAGAAGATGC
NlaIV 822 GGATCC GG[ACGT]^[ACGT]CC
949 GGTACC
TspRI 31 CAGTG CA[GC]TG[ACGT][ACGT]^
982 CAGTG
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AfaI AluI BbvI BciVI BsaJI
BsmI BsrI BssKI BstF5I BstNI
DdeI DpnII EcoRII Fnu4HI FokI
HinfI HphI ItaI MboI MboII
MnlI MseI MvaI NdeII RsaI
Sau3AI ScrFI SfaNI SmlI TaqI
TfiI Tru9I TseI Tsp509I TthHB8I