Restriction map for
pFN21AE9416
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbvCI BcgI
BfaI BfrI BlnI BsaAI BsaBI
BsaHI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I CfoI
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MscI MspA1I
MunI MvnI NarI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 625 GTCGAC GT^[AC][GT]AC
AccIII 431 TCCGGA T^CCGGA
AflIII 1292 ACATGT A^C[GA][CT]GT
AlwNI 679 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1159 GTGCTC G[AT]GC[AT]^C
BanI 1166 GGTGCC G^G[CT][GA]CC
BciVI 335 GTATCC GTATCC(6/5)
BclI 971 TGATCA T^GATCA
BcnI 339 CCCGG CC^[GC]GG
BglII 1376 AGATCT A^GATCT
BlpI 323 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 323 GCTCAGC GC^T[ACGT]AGC
BsaI 651 GGTCTC GGTCTC(1/5)
BsaWI 431 TCCGGA [AT]^CCGG[AT]
BseAI 431 TCCGGA T^CCGGA
BsiHKAI 1159 GTGCTC G[AT]GC[AT]^C
BspEI 431 TCCGGA T^CCGGA
BspLU11I 1292 ACATGT A^CATGT
BsrDI 1043 GCAATG GCAATG(2/0)
CelII 323 GCTCAGC GC^T[ACGT]AGC
DraII 761 GGGTCCC [GA]G^G[ACGT]CC[CT]
EarI 271 CTCTTC CTCTTC(1/4)
EcoO109I 761 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 1543 ATGCAT ATGCA^T
FbaI 971 TGATCA T^GATCA
Ksp632I 271 CTCTTC CTCTTC(1/4)
MaeII 845 ACGT A^CGT
MroI 431 TCCGGA T^CCGGA
MseI 1111 TTAA T^TAA
NaeI 804 GCCGGC GCC^GGC
NciI 339 CCCGG CC^[GC]GG
NcoI 1226 CCATGG C^CATGG
NgoMIV 804 GCCGGC G^CCGGC
NsiI 1543 ATGCAT ATGCA^T
NspI 1292 ACATGT [GA]CATG^[CT]
PpuMI 761 GGGTCCC [GA]G^G[AT]CC[CT]
SalI 625 GTCGAC G^TCGAC
SmlI 698 CTCAAG C^T[CT][GA]AG
StuI 1182 AGGCCT AGG^CCT
Tru9I 1111 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 148 GCGG CCGC(-3/-1)
687 GCGG
AcsI 220 AAATTC [GA]^AATT[CT]
1318 AAATTT
ApoI 220 AAATTC [GA]^AATT[CT]
1318 AAATTT
AvaII 762 GGTCC G^G[AT]CC
1326 GGACC
BglI 526 GCCACCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
799 GCCATGCCGGC
BmyI 711 GTGCCC G[AGT]GC[ACT]^C
1159 GTGCTC
BseRI 487 CTCCTC GAGGAG(10/8)
1477 GAGGAG
Bsp1286I 711 GTGCCC G[AGT]GC[ACT]^C
1159 GTGCTC
BsrFI 425 ACCGGC [GA]^CCGG[CT]
804 GCCGGC
Cac8I 804 GCCGGC GC[ACGT]^[ACGT]GC
898 GCAAGC
Cfr10I 425 ACCGGC [GA]^CCGG[CT]
804 GCCGGC
HincII 625 GTCGAC GT[CT]^[GA]AC
1249 GTCAAC
HindII 625 GTCGAC GT[CT]^[GA]AC
1249 GTCAAC
PstI 838 CTGCAG CTGCA^G
893 CTGCAG
TfiI 946 GATTC G^A[AT]TC
1602 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AfaI AluI AlwI BbsI BbvI
BpmI BpuAI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BspHI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstXI BstYI Cfr13I DdeI DpnII
DsaI EcoO65I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII