Restriction map for
pFN21AB9966
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BbeI BbrPI BcgI BciVI
BclI BcnI BglI BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
FauI FbaI FseI FspI HaeII
HapII HgaI Hin1I HpaI HpaII
KasI KpnI KspI MamI MluI
MroI MspI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflII 721 CTTAAG C^TTAAG
AocI 337 CCTGAGG CC^T[ACGT]AGG
AspHI 1606 GTGCTC G[AT]GC[AT]^C
AvaI 132 CCCGAG C^[CT]CG[GA]G
BamHI 686 GGATCC G^GATCC
BanII 42 GGGCTC G[GA]GC[CT]^C
BbsI 946 GAAGAC GAAGAC(2/6)
BbvCI 729 GCTGAGG CCTCAGC(-5/-2)
BfrI 721 CTTAAG C^TTAAG
BglII 227 AGATCT A^GATCT
BpuAI 946 GAAGAC GAAGAC(2/6)
BsaI 15 GGTCTC GGTCTC(1/5)
BseRI 1689 CTCCTC GAGGAG(10/8)
BsgI 1441 GTGCAG GTGCAG(16/14)
BsiHKAI 1606 GTGCTC G[AT]GC[AT]^C
BsmBI 843 GAGACG CGTCTC(1/5)
BsoBI 132 CCCGAG C^[CT]CG[GA]G
BstAPI 1416 GCATGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 337 CCTGAGG CC^T[ACGT]AGG
CfoI 1716 GCGC GCG^C
DraII 209 GGGACCT [GA]G^G[ACGT]CC[CT]
EarI 2016 CTCTTC CTCTTC(1/4)
Eco81I 337 CCTGAGG CC^T[ACGT]AGG
EcoO109I 209 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1052 ATGCAT ATGCA^T
HhaI 1716 GCGC GCG^C
HinP1I 1716 GCGC G^CGC
Ksp632I 2016 CTCTTC CTCTTC(1/4)
MfeI 476 CAATTG C^AATTG
MunI 476 CAATTG C^AATTG
NcoI 1556 CCATGG C^CATGG
NsiI 1052 ATGCAT ATGCA^T
NspI 1416 GCATGC [GA]CATG^[CT]
PpuMI 209 GGGACCT [GA]G^G[AT]CC[CT]
SalI 741 GTCGAC G^TCGAC
SapI 2015 GCTCTTC GCTCTTC(1/4)
SfcI 963 CTACAG C^T[GA][CT]AG
SphI 1416 GCATGC GCATG^C
StuI 341 AGGCCT AGG^CCT
XbaI 666 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 741 GTCGAC GT^[AC][GT]AC
1369 GTCTAC
AciI 1449 CCGC CCGC(-3/-1)
1668 GCGG
AcsI 486 AAATTC [GA]^AATT[CT]
1955 AAATTT
AfaI 987 GTAC GT^AC
1083 GTAC
AhdI 918 GACTAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
949 GACAATGAGTC
ApoI 486 AAATTC [GA]^AATT[CT]
1955 AAATTT
AspEI 918 GACTAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
949 GACAATGAGTC
AvaII 210 GGACC G^G[AT]CC
655 GGACC
BanI 1755 GGCACC G^G[CT][GA]CC
1794 GGTGCC
BlpI 501 GCTGAGC GC^T[ACGT]AGC
1920 GCTGAGC
BpmI 214 CTGGAG CTGGAG(16/14)
840 CTGGAG
Bpu1102I 501 GCTGAGC GC^T[ACGT]AGC
1920 GCTGAGC
BsmAI 843 GAGAC GTCTC(1/5)
16 GTCTC
CelII 501 GCTGAGC GC^T[ACGT]AGC
1920 GCTGAGC
DsaI 642 CCACGG C^C[GA][CT]GG
1556 CCATGG
Eam1105I 918 GACTAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
949 GACAATGAGTC
EcoRV 1072 GATATC GAT^ATC
1264 GATATC
HincII 741 GTCGAC GT[CT]^[GA]AC
1024 GTTGAC
HindII 741 GTCGAC GT[CT]^[GA]AC
1024 GTTGAC
HindIII 169 AAGCTT A^AGCTT
778 AAGCTT
MaeII 985 ACGT A^CGT
1028 ACGT
PflMI 1298 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1999 CCAAGGAATGG
RsaI 987 GTAC GT^AC
1083 GTAC
SmlI 721 CTTAAG C^T[CT][GA]AG
1789 CTCAAG
TaqI 174 TCGA T^CGA
742 TCGA
TthHB8I 174 TCGA T^CGA
742 TCGA
Van91I 1298 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1999 CCAAGGAATGG
XcmI 642 CCACGGAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1995 CCATCCAAGGAATGG
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AluI AlwI BalI BbvI BfaI
BmyI BsaJI BsiYI BslI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
PvuII Sau3AI Sau96I ScrFI SfaNI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII