Restriction map for
pFN21AE2330
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# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp718I AspEI AspI AviII
AvrII BbrPI BcgI BlnI BsaAI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmFI BspEI BspLU11I BsrFI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Cfr10I CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I FseI FspI HgaI HpaI
KpnI KspI MroI NaeI NcoI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SfuI
SgrAI SmaI SnaBI SphI SspI
SwaI Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccII 1834 CGCG CG^CG
AcyI 212 GGCGCC G[GA]^CG[CT]C
AflII 1495 CTTAAG C^TTAAG
AflIII 1833 ACGCGT A^C[GA][CT]GT
ApaI 3563 GGGCCC GGGCC^C
AseI 2487 ATTAAT AT^TAAT
AsnI 2487 ATTAAT AT^TAAT
AspHI 3038 GTGCTC G[AT]GC[AT]^C
AvaI 4109 CTCGAG C^[CT]CG[GA]G
BanI 212 GGCGCC G^G[CT][GA]CC
BbeI 212 GGCGCC GGCGC^C
BbvCI 1907 CCTCAGC CCTCAGC(-5/-2)
BcnI 4118 CCCGG CC^[GC]GG
BfrI 1495 CTTAAG C^TTAAG
BglII 692 AGATCT A^GATCT
BsaHI 212 GGCGCC G[GA]^CG[CT]C
BsaI 347 GAGACC GGTCTC(1/5)
BsiHKAI 3038 GTGCTC G[AT]GC[AT]^C
BsoBI 4109 CTCGAG C^[CT]CG[GA]G
BspHI 2010 TCATGA T^CATGA
BstUI 1834 CGCG CG^CG
CfoI 213 GCGC GCG^C
DraI 2958 TTTAAA TTT^AAA
DraII 3562 AGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 1678 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 3562 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRV 3636 GATATC GAT^ATC
EcoT14I 3567 CCAAGG C^C[AT][AT]GG
EcoT22I 1043 ATGCAT ATGCA^T
HaeII 212 GGCGCC [GA]GCGC^[CT]
HapII 4119 CCGG C^CGG
HhaI 213 GCGC GCG^C
Hin1I 212 GGCGCC G[GA]^CG[CT]C
HinP1I 213 GCGC G^CGC
HincII 706 GTCAAC GT[CT]^[GA]AC
HindII 706 GTCAAC GT[CT]^[GA]AC
HpaII 4119 CCGG C^CGG
KasI 212 GGCGCC G^GCGCC
MaeII 2628 ACGT A^CGT
MluI 1833 ACGCGT A^CGCGT
MspI 4119 CCGG C^CGG
MvnI 1834 CGCG CG^CG
NarI 212 GGCGCC GG^CGCC
NciI 4118 CCCGG CC^[GC]GG
NdeI 304 CATATG CA^TATG
NsiI 1043 ATGCAT ATGCA^T
NspI 1542 ACATGC [GA]CATG^[CT]
PaeR7I 4109 CTCGAG C^TCGAG
PflMI 4011 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2487 ATTAAT AT^TAAT
RcaI 2010 TCATGA T^CATGA
SbfI 2707 CCTGCAGG CCTGCA^GG
ScaI 3715 AGTACT AGT^ACT
SfiI 2887 GGCCGAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 212 GGCGCC GGC^GCC
SpeI 3865 ACTAGT A^CTAGT
Sse8387I 2707 CCTGCAGG CCTGCA^GG
StyI 3567 CCAAGG C^C[AT][AT]GG
Van91I 4011 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 4109 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AocI 606 CCTGAGG CC^T[ACGT]AGG
2328 CCTAAGG
Asp700I 793 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1651 GAAACCATTC
BalI 445 TGGCCA TGG^CCA
3476 TGGCCA
BciVI 1876 GGATAC GTATCC(6/5)
3663 GTATCC
BclI 2181 TGATCA T^GATCA
4053 TGATCA
BglI 2888 GCCGAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3265 GCCAGTTTGGC
BlpI 503 GCTTAGC GC^T[ACGT]AGC
2113 GCTGAGC
Bpu1102I 503 GCTTAGC GC^T[ACGT]AGC
2113 GCTGAGC
BsaBI 687 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1018 GATTCTCATC
BsmI 3731 GAATGC GAATGC(1/-1)
3343 GCATTC
Bsp1407I 888 TGTACA T^GTACA
3783 TGTACA
BspMI 350 ACCTGC ACCTGC(4/8)
3678 GCAGGT
BsrBI 1108 CCGCTC CCGCTC(-3/-3)
436 GAGCGG
BsrGI 888 TGTACA T^GTACA
3783 TGTACA
BssSI 3850 CACGAG CACGAG(-5/-1)
1111 CTCGTG
Bsu36I 606 CCTGAGG CC^T[ACGT]AGG
2328 CCTAAGG
CelII 503 GCTTAGC GC^T[ACGT]AGC
2113 GCTGAGC
EaeI 445 TGGCCA [CT]^GGCC[GA]
3476 TGGCCA
Eco81I 606 CCTGAGG CC^T[ACGT]AGG
2328 CCTAAGG
EcoNI 1239 CCTTTTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2704 CCTCCTGCAGG
EcoRI 797 GAATTC G^AATTC
897 GAATTC
FauI 2414 CCCGC CCCGC(4/6)
3909 CCCGC
FbaI 2181 TGATCA T^GATCA
4053 TGATCA
HindIII 717 AAGCTT A^AGCTT
2278 AAGCTT
MamI 687 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1018 GATTCTCATC
MfeI 2204 CAATTG C^AATTG
4133 CAATTG
MluNI 445 TGGCCA TGG^CCA
3476 TGGCCA
MscI 445 TGGCCA TGG^CCA
3476 TGGCCA
MunI 2204 CAATTG C^AATTG
4133 CAATTG
SapI 2677 GAAGAGC GCTCTTC(1/4)
337 GCTCTTC
SexAI 1679 ACCAGGT A^CC[AT]GGT
2160 ACCAGGT
SspBI 888 TGTACA T^GTACA
3783 TGTACA
XbaI 58 TCTAGA T^CTAGA
2841 TCTAGA
XcmI 2822 CCAGCAAAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3567 CCAAGGAGGAGATGG
XmnI 793 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1651 GAAACCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BamHI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiYI
BslI BsmAI Bsp1286I BspDI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I ClaI DdeI DpnII
DsaI EarI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII