Restriction map for
pFN21AE6759
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspI AvrII BamHI BbsI
BclI BfaI BfrI BglI BglII
BlnI BpuAI BsaBI BsaI BseAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrGI BssSI Bst1107I BstBI BstZ17I
Bsu36I ClaI DraIII DrdI Eco47III
Eco81I EcoNI EcoRV EcoT22I FbaI
HindIII HpaI KpnI KspI MaeI
MamI MfeI MluI MroI MunI
NdeI NheI NruI NsiI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI SacII SalI
SapI SbfI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AhdI 997 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 156 GGGCCC GGGCC^C
Asp700I 841 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 997 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 505 CACGTG CAC^GTG
BcgI 392 GCATGGCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1044 GTATCC GTATCC(6/5)
BpmI 1173 CTCCAG CTGGAG(16/14)
BsaAI 505 CACGTG [CT]AC^GT[GA]
BsaWI 140 TCCGGT [AT]^CCGG[AT]
BspMI 1130 ACCTGC ACCTGC(4/8)
BstAPI 1017 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1117 GGTGACC G^GT[ACGT]ACC
BstPI 1117 GGTGACC G^GT[ACGT]ACC
BstXI 1291 CCAGTGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 257 AGATCC [GA]^GATC[CT]
CpoI 142 CGGTCCG CG^G[AT]CCG
DraI 1546 TTTAAA TTT^AAA
Eam1105I 997 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1117 GGTGACC G^GT[ACGT]ACC
EcoRI 778 GAATTC G^AATTC
FseI 350 GGCCGGCC GGCCGG^CC
HgaI 880 GACGC GACGC(5/10)
HincII 1264 GTCAAC GT[CT]^[GA]AC
HindII 1264 GTCAAC GT[CT]^[GA]AC
MflI 257 AGATCC [GA]^GATC[CT]
NaeI 351 GCCGGC GCC^GGC
NcoI 1574 CCATGG C^CATGG
NgoMIV 351 GCCGGC G^CCGGC
NotI 383 GCGGCCGC GC^GGCCGC
NspI 99 GCATGT [GA]CATG^[CT]
PflMI 312 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 505 CACGTG CAC^GTG
PmlI 505 CACGTG CAC^GTG
PpuMI 1361 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 270 CAGCTG CAG^CTG
RsrII 142 CGGTCCG CG^G[AT]CCG
SacI 1579 GAGCTC GAGCT^C
ScaI 743 AGTACT AGT^ACT
SexAI 1039 ACCAGGT A^CC[AT]GGT
Van91I 312 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 257 AGATCC [GA]^GATC[CT]
XmnI 841 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AfaI 744 GTAC GT^AC
958 GTAC
AspHI 415 GAGCAC G[AT]GC[AT]^C
1579 GAGCTC
AviII 455 TGCGCA TGC^GCA
761 TGCGCA
BanI 562 GGCGCC G^G[CT][GA]CC
985 GGCGCC
BbeI 562 GGCGCC GGCGC^C
985 GGCGCC
BbvCI 12 CCTCAGC CCTCAGC(-5/-2)
117 GCTGAGG
BlpI 510 GCTGAGC GC^T[ACGT]AGC
672 GCTCAGC
Bpu1102I 510 GCTGAGC GC^T[ACGT]AGC
672 GCTCAGC
BseRI 1027 CTCCTC GAGGAG(10/8)
1030 CTCCTC
BsiHKAI 415 GAGCAC G[AT]GC[AT]^C
1579 GAGCTC
BspHI 611 TCATGA T^CATGA
1284 TCATGA
CelII 510 GCTGAGC GC^T[ACGT]AGC
672 GCTCAGC
DraII 314 AGGCCCT [GA]G^G[ACGT]CC[CT]
1361 AGGACCT
DsaI 599 CCGTGG C^C[GA][CT]GG
1574 CCATGG
EcoO109I 314 AGGCCCT [GA]G^G[ACGT]CC[CT]
1361 AGGACCT
FspI 455 TGCGCA TGC^GCA
761 TGCGCA
KasI 562 GGCGCC G^GCGCC
985 GGCGCC
MseI 1532 TTAA T^TAA
1547 TTAA
MslI 279 CATAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
925 CACGGTGGTG
NarI 562 GGCGCC GG^CGCC
985 GGCGCC
PaeR7I 496 CTCGAG C^TCGAG
1061 CTCGAG
PstI 739 CTGCAG CTGCA^G
1015 CTGCAG
RcaI 611 TCATGA T^CATGA
1284 TCATGA
RsaI 744 GTAC GT^AC
958 GTAC
SfoI 562 GGCGCC GGC^GCC
985 GGCGCC
SmaI 153 CCCGGG CCC^GGG
853 CCCGGG
TfiI 1281 GAATC G^A[AT]TC
1331 GATTC
Tru9I 1532 TTAA T^TAA
1547 TTAA
XcmI 92 CCACTGTGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1244 CCAAGGACACTGTGG
XhoI 496 CTCGAG C^TCGAG
1061 CTCGAG
XmaCI 153 CCCGGG C^CCGGG
853 CCCGGG
XmaI 153 CCCGGG C^CCGGG
853 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
AlwI AlwNI ApoI AvaI AvaII
BalI BanII BbvI BcnI BmyI
BsaHI BsaJI BsgI BsiEI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII EaeI EagI EarI
EclXI Eco52I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I