Restriction map for
pFN21AE6368
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII AccI AccIII AcyI AflII
AflIII Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
AviII AvrII BbeI BbrPI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BseAI BsgI BsiEI
BsiWI BsmFI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KspI MaeI MamI
MluI MroI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflMI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PstI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 70 enzymes) #
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Acc65I 347 GGTACC G^GTACC
AccII 22 CGCG CG^CG
AclI 765 AACGTT AA^CGTT
AfaI 348 GTAC GT^AC
AgeI 350 ACCGGT A^CCGGT
AhdI 398 GACATTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 347 GGTACC G^GTACC
AspEI 398 GACATTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 752 GAGCTC G[AT]GC[AT]^C
AspI 301 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 234 CCCGGG C^[CT]CG[GA]G
AvaII 405 GGTCC G^G[AT]CC
BalI 106 TGGCCA TGG^CCA
BamHI 238 GGATCC G^GATCC
BanII 752 GAGCTC G[GA]GC[CT]^C
BbsI 11 GAAGAC GAAGAC(2/6)
BbvCI 98 CCTCAGC CCTCAGC(-5/-2)
BpuAI 11 GAAGAC GAAGAC(2/6)
BsaWI 350 ACCGGT [AT]^CCGG[AT]
BsiHKAI 752 GAGCTC G[AT]GC[AT]^C
BsmBI 965 CGTCTC CGTCTC(1/5)
BsoBI 234 CCCGGG C^[CT]CG[GA]G
BspHI 834 TCATGA T^CATGA
BspMI 41 ACCTGC ACCTGC(4/8)
BssSI 512 CTCGTG CACGAG(-5/-1)
BstAPI 267 GCACATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 802 TTCGAA TT^CGAA
BstUI 22 CGCG CG^CG
BstXI 708 CCAACTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 238 GGATCC [GA]^GATC[CT]
CfoI 21 GCGC GCG^C
Cfr13I 405 GGTCC G^G[ACGT]CC
DsaI 992 CCATGG C^C[GA][CT]GG
Eam1105I 398 GACATTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 992 CCATGG C^C[AT][AT]GG
FauI 678 CCCGC CCCGC(4/6)
HgaI 23 GCGTC GACGC(5/10)
HhaI 21 GCGC GCG^C
HinP1I 21 GCGC G^CGC
HphI 598 GGTGA GGTGA(8/7)
KpnI 347 GGTACC GGTAC^C
MaeII 766 ACGT A^CGT
MaeIII 573 GTCAC ^GT[ACGT]AC
MfeI 818 CAATTG C^AATTG
MflI 238 GGATCC [GA]^GATC[CT]
MluNI 106 TGGCCA TGG^CCA
MscI 106 TGGCCA TGG^CCA
MspA1I 580 CAGCTG C[AC]G^C[GT]G
MunI 818 CAATTG C^AATTG
MvnI 22 CGCG CG^CG
NcoI 992 CCATGG C^CATGG
NspV 802 TTCGAA TT^CGAA
PflFI 301 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 350 ACCGGT A^CCGGT
Psp1406I 765 AACGTT AA^CGTT
PvuII 580 CAGCTG CAG^CTG
RcaI 834 TCATGA T^CATGA
RsaI 348 GTAC GT^AC
SacI 752 GAGCTC GAGCT^C
SapI 154 GCTCTTC GCTCTTC(1/4)
Sau96I 405 GGTCC G^G[ACGT]CC
SfuI 802 TTCGAA TT^CGAA
SgrAI 16 CACCGGCG C[GA]^CCGG[CT]G
SmaI 234 CCCGGG CCC^GGG
StyI 992 CCATGG C^C[AT][AT]GG
Tsp45I 573 GTCAC ^GT[GC]AC
Tth111I 301 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 238 GGATCC [GA]^GATC[CT]
XmaCI 234 CCCGGG C^CCGGG
XmaI 234 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Asp700I 805 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1069 GAATATCTTC
BcnI 234 CCCGG CC^[GC]GG
235 CCGGG
BmyI 30 GGGCAC G[AGT]GC[ACT]^C
752 GAGCTC
BpmI 577 CTCCAG CTGGAG(16/14)
689 CTGGAG
BsaI 620 GGTCTC GGTCTC(1/5)
644 GGTCTC
BseRI 623 CTCCTC GAGGAG(10/8)
112 GAGGAG
Bsp1286I 30 GGGCAC G[AGT]GC[ACT]^C
752 GAGCTC
BsrDI 215 CATTGC GCAATG(2/0)
493 GCAATG
BsrFI 17 ACCGGC [GA]^CCGG[CT]
350 ACCGGT
Cfr10I 17 ACCGGC [GA]^CCGG[CT]
350 ACCGGT
EaeI 56 TGGCCG [CT]^GGCC[GA]
106 TGGCCA
EcoRI 144 GAATTC G^AATTC
281 GAATTC
HaeIII 57 GGCC GG^CC
107 GGCC
MseI 541 TTAA T^TAA
931 TTAA
NciI 234 CCCGG CC^[GC]GG
235 CCGGG
Tru9I 541 TTAA T^TAA
931 TTAA
XmnI 805 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1069 GAATATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BanI BbvI BsaJI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
Cac8I DdeI DpnII EarI EcoRII
Fnu4HI FokI HapII HinfI HpaII
ItaI Ksp632I MboI MboII MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI ScrFI SfaNI
SmlI TaqI TfiI TseI Tsp509I
TspRI TthHB8I