Restriction map for
pFN21AE3207
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspI AviII AvrII BbeI BbrPI
BcgI BciVI BclI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspHI BspLU11I BsrDI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoT22I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI MfeI
MluI MroI MunI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI PvuII RcaI RsrII
SalI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccII 19 CGCG CG^CG
AflII 1048 CTTAAG C^TTAAG
AhdI 234 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 703 ATTAAT AT^TAAT
AsnI 703 ATTAAT AT^TAAT
AspEI 234 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 916 TGGCCA TGG^CCA
BanI 14 GGTGCC G^G[CT][GA]CC
BbvCI 630 GCTGAGG CCTCAGC(-5/-2)
BbvI 276 GCTGC GCAGC(8/12)
BcnI 589 CCCGG CC^[GC]GG
BfrI 1048 CTTAAG C^TTAAG
BsaBI 723 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 70 CTGCAC GTGCAG(16/14)
BsmBI 414 CGTCTC CGTCTC(1/5)
BsmI 1320 GCATTC GAATGC(1/-1)
Bsp1407I 1248 TGTACA T^GTACA
BsrBI 315 GAGCGG CCGCTC(-3/-3)
BsrFI 329 GCCGGT [GA]^CCGG[CT]
BsrGI 1248 TGTACA T^GTACA
BssSI 160 CACGAG CACGAG(-5/-1)
BstAPI 621 GCAGTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 19 CGCG CG^CG
BstXI 1363 CCAGTACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 329 GCCGGT [GA]^CCGG[CT]
DraII 121 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 18 CCGCGG C^C[GA][CT]GG
Eam1105I 234 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 121 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1115 GAATTC G^AATTC
EcoRV 727 GATATC GAT^ATC
EcoT14I 644 CCTTGG C^C[AT][AT]GG
FauI 279 GCGGG CCCGC(4/6)
KspI 18 CCGCGG CCGC^GG
MaeII 413 ACGT A^CGT
MamI 723 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 916 TGGCCA TGG^CCA
MscI 916 TGGCCA TGG^CCA
MspA1I 18 CCGCGG C[AC]G^C[GT]G
MvnI 19 CGCG CG^CG
NciI 589 CCCGG CC^[GC]GG
NdeI 1334 CATATG CA^TATG
NspI 626 ACATGC [GA]CATG^[CT]
PflMI 849 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 121 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 703 ATTAAT AT^TAAT
SacII 18 CCGCGG CCGC^GG
SapI 403 GAAGAGC GCTCTTC(1/4)
SexAI 1377 ACCTGGT A^CC[AT]GGT
SspBI 1248 TGTACA T^GTACA
StyI 644 CCTTGG C^C[AT][AT]GG
TseI 276 GCTGC G^C[AT]GC
Van91I 849 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 595 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
764 CAGGCTCTG
Asp700I 56 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
522 GAACAACTTC
BamHI 352 GGATCC G^GATCC
360 GGATCC
BbsI 649 GAAGAC GAAGAC(2/6)
964 GAAGAC
BpuAI 649 GAAGAC GAAGAC(2/6)
964 GAAGAC
BseRI 603 GAGGAG GAGGAG(10/8)
633 GAGGAG
BspMI 137 ACCTGC ACCTGC(4/8)
119 GCAGGT
BstF5I 238 GGATG GGATG(2/0)
1302 GGATG
BstYI 352 GGATCC [GA]^GATC[CT]
360 GGATCC
EaeI 591 CGGCCA [CT]^GGCC[GA]
916 TGGCCA
EcoNI 39 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
78 CCTGGGGCAGG
FokI 238 GGATG GGATG(9/13)
1302 GGATG
HapII 330 CCGG C^CGG
590 CCGG
HpaII 330 CCGG C^CGG
590 CCGG
MflI 352 GGATCC [GA]^GATC[CT]
360 GGATCC
MslI 877 CACTGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1054 CATGGTGGTG
MspI 330 CCGG C^CGG
590 CCGG
SacI 101 GAGCTC GAGCT^C
301 GAGCTC
SfaNI 369 GATGC GCATC(5/9)
218 GCATC
XhoII 352 GGATCC [GA]^GATC[CT]
360 GGATCC
XmnI 56 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
522 GAACAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BanII
BfaI BmyI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI HaeIII HgaI HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PaeR7I PleI
RsaI Sau3AI Sau96I ScrFI SmlI
TaqI TfiI Tru9I Tsp45I Tsp509I
TspRI TthHB8I XhoI