Restriction map for
pFN21AE3129
##################################
# NO CUT ENZYMES ( 161 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AvaI AvaII
AviII AvrII BalI BbeI BbrPI
BbvCI BcgI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HapII HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 204 GACGTC GACGT^C
AocI 704 CCTTAGG CC^T[ACGT]AGG
Aor51HI 637 AGCGCT AGC^GCT
Asp700I 320 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 79 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 555 GGATCC G^GATCC
BanI 163 GGTGCC G^G[CT][GA]CC
BbsI 862 GAAGAC GAAGAC(2/6)
BciVI 786 GGATAC GTATCC(6/5)
BpuAI 862 GAAGAC GAAGAC(2/6)
BseRI 37 GAGGAG GAGGAG(10/8)
BstXI 437 CCAAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 704 CCTTAGG CC^T[ACGT]AGG
CfoI 638 GCGC GCG^C
Cfr13I 498 GGCCC G^G[ACGT]CC
Eco47III 637 AGCGCT AGC^GCT
Eco81I 704 CCTTAGG CC^T[ACGT]AGG
FauI 500 CCCGC CCCGC(4/6)
HaeII 637 AGCGCT [GA]GCGC^[CT]
HgaI 7 GCGTC GACGC(5/10)
HhaI 638 GCGC GCG^C
HinP1I 638 GCGC G^CGC
HinfI 232 GATTC G^A[ACGT]TC
HphI 802 GGTGA GGTGA(8/7)
MaeII 205 ACGT A^CGT
MboII 862 GAAGA GAAGA(8/7)
NspI 866 ACATGC [GA]CATG^[CT]
PflFI 79 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 106 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 818 GAGCTC GAGCT^C
Sau96I 498 GGCCC G^G[ACGT]CC
SexAI 80 ACCTGGT A^CC[AT]GGT
TaqI 458 TCGA T^CGA
TfiI 232 GATTC G^A[AT]TC
Tth111I 79 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 458 TCGA T^CGA
Van91I 106 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 542 CCATTGCTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 320 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 286 GAATTT [GA]^AATT[CT]
381 AAATTT
AcyI 6 GGCGTC G[GA]^CG[CT]C
204 GACGTC
ApoI 286 GAATTT [GA]^AATT[CT]
381 AAATTT
AspHI 667 GTGCTC G[AT]GC[AT]^C
818 GAGCTC
BanII 63 GGGCTC G[GA]GC[CT]^C
818 GAGCTC
BsaHI 6 GGCGTC G[GA]^CG[CT]C
204 GACGTC
BsiHKAI 667 GTGCTC G[AT]GC[AT]^C
818 GAGCTC
BstF5I 123 CATCC GGATG(2/0)
718 GGATG
Cac8I 452 GCCAGC GC[ACGT]^[ACGT]GC
499 GCCCGC
FokI 123 CATCC GGATG(9/13)
718 GGATG
Hin1I 6 GGCGTC G[GA]^CG[CT]C
204 GACGTC
MaeIII 732 GTCAC ^GT[ACGT]AC
803 GTGAC
MseI 262 TTAA T^TAA
616 TTAA
MwoI 640 GCTGCAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
649 GCTGGGGAGGC
NcoI 126 CCATGG C^CATGG
209 CCATGG
PstI 641 CTGCAG CTGCA^G
911 CTGCAG
SfaNI 307 GATGC GCATC(5/9)
646 GATGC
Tru9I 262 TTAA T^TAA
616 TTAA
Tsp45I 732 GTCAC ^GT[GC]AC
803 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AlwI AlwNI BbvI
BmyI BpmI BsaJI BsiYI BslI
Bsp1286I BsrDI BsrI BssKI BstNI
BstYI DdeI DpnII DsaI EcoRII
EcoT14I Fnu4HI HaeIII ItaI MboI
MflI MnlI MvaI NdeII NlaIII
NlaIV Sau3AI ScrFI SfcI StyI
TseI Tsp509I TspRI XhoII