Restriction map for
pFN21AE6448
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspI AviII
AvrII BamHI BbeI BbrPI BcgI
BclI BfrI BglII BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraI DrdI Eco47III EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 1222 CGCG CG^CG
AhdI 256 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 124 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 660 CCTGAGG CC^T[ACGT]AGG
AspEI 256 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1560 GAGCTC G[AT]GC[AT]^C
BbvCI 557 CCTCAGC CCTCAGC(-5/-2)
BciVI 57 GGATAC GTATCC(6/5)
BfaI 118 CTAG C^TAG
BglI 432 GCCCCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 249 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 249 GCTGAGC GC^T[ACGT]AGC
BsaI 1507 GGTCTC GGTCTC(1/5)
BsgI 462 CTGCAC GTGCAG(16/14)
BsiEI 273 CGGCCG CG[GA][CT]^CG
BsiHKAI 1560 GAGCTC G[AT]GC[AT]^C
BstAPI 280 GCACTAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1222 CGCG CG^CG
BstYI 126 GGATCT [GA]^GATC[CT]
Bsu36I 660 CCTGAGG CC^T[ACGT]AGG
CelII 249 GCTGAGC GC^T[ACGT]AGC
EagI 273 CGGCCG C^GGCCG
Eam1105I 256 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 273 CGGCCG C^GGCCG
Eco52I 273 CGGCCG C^GGCCG
Eco81I 660 CCTGAGG CC^T[ACGT]AGG
EcoT14I 937 CCTTGG C^C[AT][AT]GG
HincII 571 GTCAAC GT[CT]^[GA]AC
HindII 571 GTCAAC GT[CT]^[GA]AC
MaeI 118 CTAG C^TAG
MaeII 1380 ACGT A^CGT
MflI 126 GGATCT [GA]^GATC[CT]
MvnI 1222 CGCG CG^CG
PaeR7I 483 CTCGAG C^TCGAG
PpuMI 81 AGGACCC [GA]G^G[AT]CC[CT]
PstI 642 CTGCAG CTGCA^G
SacI 1560 GAGCTC GAGCT^C
SexAI 837 ACCAGGT A^CC[AT]GGT
SmaI 85 CCCGGG CCC^GGG
SphI 1016 GCATGC GCATG^C
StuI 229 AGGCCT AGG^CCT
StyI 937 CCTTGG C^C[AT][AT]GG
XcmI 1470 CCAGAAGGTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 483 CTCGAG C^TCGAG
XhoII 126 GGATCT [GA]^GATC[CT]
XmaCI 85 CCCGGG C^CCGGG
XmaI 85 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 126 GGATC GGATC(4/5)
907 GGATC
BalI 1283 TGGCCA TGG^CCA
1482 TGGCCA
BbsI 64 GAAGAC GAAGAC(2/6)
1137 GAAGAC
BpuAI 64 GAAGAC GAAGAC(2/6)
1137 GAAGAC
BspMI 943 ACCTGC ACCTGC(4/8)
1426 ACCTGC
BstF5I 1308 CATCC GGATG(2/0)
1619 CATCC
BstXI 360 CCAGCTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1115 CCAACCACCTGG
DpnII 127 GATC ^GATC
908 GATC
EarI 145 GAAGAG CTCTTC(1/4)
151 GAAGAG
FokI 1308 CATCC GGATG(9/13)
1619 CATCC
HaeII 1010 GGCGCT [GA]GCGC^[CT]
1463 AGCGCC
HgaI 1223 GCGTC GACGC(5/10)
1296 GCGTC
Ksp632I 145 GAAGAG CTCTTC(1/4)
151 GAAGAG
MboI 127 GATC ^GATC
908 GATC
MluNI 1283 TGGCCA TGG^CCA
1482 TGGCCA
MscI 1283 TGGCCA TGG^CCA
1482 TGGCCA
NdeII 127 GATC ^GATC
908 GATC
Sau3AI 127 GATC ^GATC
908 GATC
SmlI 483 CTCGAG C^T[CT][GA]AG
1291 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI ApaI Asp700I
AvaI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI DraII DraIII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboII MnlI MslI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
NspI PflMI PleI PvuII RsaI
Sau96I ScrFI SfaNI SfcI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XmnI