Restriction map for
pFN21AE6775
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BbeI BbrPI
BcgI BclI BfrI BlnI BlpI
Bpu1102I BsaAI BsaHI BseAI BseRI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII CfoI ClaI
DraI DraIII Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KspI MaeII
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI SacI SacII SalI SbfI
ScaI SexAI SfoI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SwaI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 839 GGTACC G^GTACC
AccII 948 CGCG CG^CG
Asp718I 839 GGTACC G^GTACC
BamHI 738 GGATCC G^GATCC
BanI 839 GGTACC G^G[CT][GA]CC
BbvCI 20 CCTCAGC CCTCAGC(-5/-2)
BciVI 963 GTATCC GTATCC(6/5)
BfaI 1231 CTAG C^TAG
BglII 272 AGATCT A^GATCT
BsaI 1894 GAGACC GGTCTC(1/5)
BsaWI 207 TCCGGT [AT]^CCGG[AT]
BsiEI 714 CGGCCG CG[GA][CT]^CG
BspHI 794 TCATGA T^CATGA
BsrDI 1065 CATTGC GCAATG(2/0)
BstUI 948 CGCG CG^CG
BstXI 1838 CCATGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 185 CGGACCG CG^G[AT]CCG
DrdI 1000 GACCAGTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 714 CGGCCG C^GGCCG
EclXI 714 CGGCCG C^GGCCG
Eco52I 714 CGGCCG C^GGCCG
FauI 851 CCCGC CCCGC(4/6)
KpnI 839 GGTACC GGTAC^C
MaeI 1231 CTAG C^TAG
MseI 1112 TTAA T^TAA
MvnI 948 CGCG CG^CG
NaeI 155 GCCGGC GCC^GGC
NcoI 1838 CCATGG C^CATGG
NgoMIV 155 GCCGGC G^CCGGC
PflMI 615 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1319 GACTC GAGTC(4/5)
PshAI 618 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 640 CAGCTG CAG^CTG
RcaI 794 TCATGA T^CATGA
RsrII 185 CGGACCG CG^G[AT]CCG
SapI 1059 GCTCTTC GCTCTTC(1/4)
SfiI 1039 GGCCTGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1178 CCCGGG CCC^GGG
SphI 1445 GCATGC GCATG^C
SspI 293 AATATT AAT^ATT
Tru9I 1112 TTAA T^TAA
Tsp509I 1114 AATT ^AATT
Van91I 615 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1178 CCCGGG C^CCGGG
XmaI 1178 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 63 GTCTAC GT^[AC][GT]AC
1637 GTCTAC
AlwNI 509 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
616 CAGACACTG
BalI 227 TGGCCA TGG^CCA
248 TGGCCA
BbsI 1138 GAAGAC GAAGAC(2/6)
790 GTCTTC
BcnI 1178 CCCGG CC^[GC]GG
1179 CCGGG
BpuAI 1138 GAAGAC GAAGAC(2/6)
790 GTCTTC
BsaBI 456 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1407 GATTGGCATC
BsgI 338 CTGCAC GTGCAG(16/14)
832 GTGCAG
BsmAI 1826 GAGAC GTCTC(1/5)
1894 GAGAC
BsmI 1652 GAATGC GAATGC(1/-1)
974 GCATTC
BsrFI 155 GCCGGC [GA]^CCGG[CT]
1661 GCCGGT
Cfr10I 155 GCCGGC [GA]^CCGG[CT]
1661 GCCGGT
MamI 456 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1407 GATTGGCATC
MluNI 227 TGGCCA TGG^CCA
248 TGGCCA
MscI 227 TGGCCA TGG^CCA
248 TGGCCA
NciI 1178 CCCGG CC^[GC]GG
1179 CCGGG
NspI 59 GCATGT [GA]CATG^[CT]
1445 GCATGC
PpuMI 286 GGGACCC [GA]G^G[AT]CC[CT]
1260 GGGACCT
StuI 1047 AGGCCT AGG^CCT
1200 AGGCCT
TfiI 407 GATTC G^A[AT]TC
1146 GATTC
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BanII BbvI BglI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XcmI XhoII