Restriction map for
pFN21AE9249
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaII AviII AvrII BalI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HpaI KasI KpnI KspI
MaeII MamI MluI MluNI MroI
MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfaNI SfcI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TfiI Tsp45I
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 8 CGCG CG^CG
AcsI 229 AAATTC [GA]^AATT[CT]
AcyI 322 GACGCC G[GA]^CG[CT]C
AocI 95 CCTTAGG CC^T[ACGT]AGG
ApoI 229 AAATTC [GA]^AATT[CT]
Asp700I 295 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 168 GAGCAC G[AT]GC[AT]^C
BamHI 392 GGATCC G^GATCC
BfaI 304 CTAG C^TAG
BglI 268 GCCCTATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 322 GACGCC G[GA]^CG[CT]C
BsiHKAI 168 GAGCAC G[AT]GC[AT]^C
BsmAI 129 GAGAC GTCTC(1/5)
BsmFI 411 GGGAC GGGAC(10/14)
BsrI 381 ACTGG ACTGG(1/-1)
BssSI 236 CTCGTG CACGAG(-5/-1)
BstF5I 372 GGATG GGATG(2/0)
BstUI 8 CGCG CG^CG
Bsu36I 95 CCTTAGG CC^T[ACGT]AGG
DdeI 96 CTTAG C^T[ACGT]AG
Eco81I 95 CCTTAGG CC^T[ACGT]AGG
FokI 372 GGATG GGATG(9/13)
HaeII 100 GGCGCT [GA]GCGC^[CT]
HgaI 322 GACGC GACGC(5/10)
Hin1I 322 GACGCC G[GA]^CG[CT]C
HindIII 157 AAGCTT A^AGCTT
HinfI 413 GACTC G^A[ACGT]TC
HphI 335 TCACC GGTGA(8/7)
MaeI 304 CTAG C^TAG
MaeIII 355 GTTAC ^GT[ACGT]AC
MfeI 252 CAATTG C^AATTG
MseI 227 TTAA T^TAA
MunI 252 CAATTG C^AATTG
MvnI 8 CGCG CG^CG
NlaIII 343 CATG CATG^
NspI 342 ACATGC [GA]CATG^[CT]
PleI 413 GACTC GAGTC(4/5)
SapI 165 GAAGAGC GCTCTTC(1/4)
SfiI 267 GGCCCTATTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 403 CTTGAG C^T[CT][GA]AG
Tru9I 227 TTAA T^TAA
XmnI 295 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AluI 158 AGCT AG^CT
191 AGCT
ApaI 3 GGGCCC GGGCC^C
87 GGGCCC
BstNI 207 CCTGG CC^[AT]GG
264 CCAGG
BstYI 149 AGATCC [GA]^GATC[CT]
392 GGATCC
DpnII 150 GATC ^GATC
393 GATC
EarI 361 CTCTTC CTCTTC(1/4)
165 GAAGAG
EcoNI 279 CCTACAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
402 CCTTGAGGAGG
EcoRII 207 CCTGG ^CC[AT]GG
264 CCAGG
FauI 6 CCCGC CCCGC(4/6)
84 GCGGG
Ksp632I 361 CTCTTC CTCTTC(1/4)
165 GAAGAG
MboI 150 GATC ^GATC
393 GATC
MboII 165 GAAGA GAAGA(8/7)
362 TCTTC
MflI 149 AGATCC [GA]^GATC[CT]
392 GGATCC
MvaI 207 CCTGG CC^[AT]GG
264 CCAGG
NdeII 150 GATC ^GATC
393 GATC
Sau3AI 150 GATC ^GATC
393 GATC
Tsp509I 230 AATT ^AATT
253 AATT
XhoII 149 AGATCC [GA]^GATC[CT]
392 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AlwI AvaI BanII BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI BsoBI Bsp1286I BssKI Cac8I
CfoI Cfr13I DraII EcoO109I Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
ItaI MnlI MspI MwoI NciI
NlaIV Sau96I ScrFI TseI