Restriction map for
pFN21AE1817
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BanI BbeI BbrPI BbsI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HgaI Hin1I
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AflIII 201 ACATGT A^C[GA][CT]GT
Alw44I 10 GTGCAC G^TGCAC
AlwNI 557 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 10 GTGCAC G^TGCAC
Asp700I 834 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 10 GTGCAC G[AT]GC[AT]^C
AvaI 72 CCCGGG C^[CT]CG[GA]G
AvaII 31 GGACC G^G[AT]CC
BalI 59 TGGCCA TGG^CCA
BamHI 655 GGATCC G^GATCC
BbvCI 795 GCTGAGG CCTCAGC(-5/-2)
BclI 782 TGATCA T^GATCA
BsaAI 46 TACGTG [CT]AC^GT[GA]
BsaWI 33 ACCGGA [AT]^CCGG[AT]
BsiHKAI 10 GTGCAC G[AT]GC[AT]^C
BsmI 834 GAATGC GAATGC(1/-1)
BsoBI 72 CCCGGG C^[CT]CG[GA]G
BspLU11I 201 ACATGT A^CATGT
BsrFI 894 GCCGGT [GA]^CCGG[CT]
BstAPI 849 GCAAATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 364 CCAGAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 655 GGATCC [GA]^GATC[CT]
CfoI 110 GCGC GCG^C
Cfr10I 894 GCCGGT [GA]^CCGG[CT]
EcoT14I 257 CCATGG C^C[AT][AT]GG
FbaI 782 TGATCA T^GATCA
HaeII 109 AGCGCC [GA]GCGC^[CT]
HhaI 110 GCGC GCG^C
HinP1I 110 GCGC G^CGC
HincII 676 GTCAAC GT[CT]^[GA]AC
HindII 676 GTCAAC GT[CT]^[GA]AC
MflI 655 GGATCC [GA]^GATC[CT]
MluNI 59 TGGCCA TGG^CCA
MscI 59 TGGCCA TGG^CCA
NcoI 257 CCATGG C^CATGG
NspI 201 ACATGT [GA]CATG^[CT]
PstI 353 CTGCAG CTGCA^G
SfaNI 193 GCATC GCATC(5/9)
SfcI 353 CTGCAG C^T[GA][CT]AG
SmaI 72 CCCGGG CCC^GGG
SmlI 187 CTTGAG C^T[CT][GA]AG
StuI 368 AGGCCT AGG^CCT
StyI 257 CCATGG C^C[AT][AT]GG
TaqI 699 TCGA T^CGA
TthHB8I 699 TCGA T^CGA
XbaI 639 TCTAGA T^CTAGA
XcmI 170 CCAGCAACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 655 GGATCC [GA]^GATC[CT]
XmaCI 72 CCCGGG C^CCGGG
XmaI 72 CCCGGG C^CCGGG
XmnI 834 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 607 AAATTC [GA]^AATT[CT]
851 AAATTC
AlwI 656 GATCC GGATC(4/5)
655 GGATC
ApaI 742 GGGCCC GGGCC^C
876 GGGCCC
ApoI 607 AAATTC [GA]^AATT[CT]
851 AAATTC
BfaI 640 CTAG C^TAG
803 CTAG
BseRI 313 CTCCTC GAGGAG(10/8)
725 GAGGAG
BsmAI 138 GAGAC GTCTC(1/5)
422 GAGAC
BstF5I 360 CATCC GGATG(2/0)
485 GGATG
DsaI 257 CCATGG C^C[GA][CT]GG
869 CCACGG
EaeI 59 TGGCCA [CT]^GGCC[GA]
104 CGGCCA
EcoNI 90 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
342 CCTGACCCAGG
FokI 360 CATCC GGATG(9/13)
485 GGATG
HindIII 529 AAGCTT A^AGCTT
547 AAGCTT
HinfI 566 GATTC G^A[ACGT]TC
908 GAATC
MaeI 640 CTAG C^TAG
803 CTAG
MslI 252 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
480 CACCCGGATG
TfiI 566 GATTC G^A[AT]TC
908 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI BanII BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI HaeIII
HapII HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PvuII RsaI
Sau3AI Sau96I ScrFI Tru9I TseI
Tsp45I Tsp509I TspRI