Restriction map for
pFN21AE6605
##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BalI
BamHI BbrPI BciVI BclI BfaI
BfrI BlnI BsaAI BsaBI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI MaeI MamI MfeI MluI
MluNI MroI MscI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SalI SapI SbfI
ScaI SfcI SfiI SfuI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TaqI Tru9I Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AlwI 108 GATCC GGATC(4/5)
Aor51HI 482 AGCGCT AGC^GCT
AspHI 403 GAGCTC G[AT]GC[AT]^C
AvaI 741 CTCGGG C^[CT]CG[GA]G
AviII 353 TGCGCA TGC^GCA
BbsI 601 GTCTTC GAAGAC(2/6)
BbvCI 73 CCTCAGC CCTCAGC(-5/-2)
BcgI 356 GCAAGGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 163 CCCGG CC^[GC]GG
BglII 455 AGATCT A^GATCT
BlpI 528 GCTGAGC GC^T[ACGT]AGC
BpmI 622 CTGGAG CTGGAG(16/14)
Bpu1102I 528 GCTGAGC GC^T[ACGT]AGC
BpuAI 601 GTCTTC GAAGAC(2/6)
BsaI 384 GGTCTC GGTCTC(1/5)
BsaWI 289 TCCGGT [AT]^CCGG[AT]
BsiHKAI 403 GAGCTC G[AT]GC[AT]^C
BsmBI 442 GAGACG CGTCTC(1/5)
BsmI 420 GAATGC GAATGC(1/-1)
BsoBI 741 CTCGGG C^[CT]CG[GA]G
BspMI 461 ACCTGC ACCTGC(4/8)
CelII 528 GCTGAGC GC^T[ACGT]AGC
DraII 300 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 566 TGGCCG [CT]^GGCC[GA]
EarI 176 GAAGAG CTCTTC(1/4)
Eco47III 482 AGCGCT AGC^GCT
EcoNI 621 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 300 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 539 CCAAGG C^C[AT][AT]GG
FspI 353 TGCGCA TGC^GCA
Ksp632I 176 GAAGAG CTCTTC(1/4)
KspI 516 CCGCGG CCGC^GG
MaeII 758 ACGT A^CGT
NciI 163 CCCGG CC^[GC]GG
NspI 533 GCATGC [GA]CATG^[CT]
PleI 179 GAGTC GAGTC(4/5)
PpuMI 300 AGGACCC [GA]G^G[AT]CC[CT]
SacI 403 GAGCTC GAGCT^C
SacII 516 CCGCGG CCGC^GG
SexAI 380 ACCAGGT A^CC[AT]GGT
SgrAI 429 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 406 CTCAAG C^T[CT][GA]AG
SphI 533 GCATGC GCATG^C
StuI 327 AGGCCT AGG^CCT
StyI 539 CCAAGG C^C[AT][AT]GG
TfiI 286 GATTC G^A[AT]TC
Tsp509I 252 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 645 GTAC GT^AC
780 GTAC
BanI 157 GGCGCC G^G[CT][GA]CC
634 GGCGCC
BanII 403 GAGCTC G[GA]GC[CT]^C
694 GAGCCC
BbeI 157 GGCGCC GGCGC^C
634 GGCGCC
BglI 515 GCCGCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
586 GCCGGCCTGGC
BseRI 475 GAGGAG GAGGAG(10/8)
715 GAGGAG
BsgI 103 GTGCAG GTGCAG(16/14)
342 GTGCAG
BstF5I 70 CATCC GGATG(2/0)
132 GGATG
BstYI 107 AGATCC [GA]^GATC[CT]
455 AGATCT
DpnII 108 GATC ^GATC
456 GATC
DrdI 302 GACCCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
376 GACAACCAGGTC
DsaI 188 CCACGG C^C[GA][CT]GG
516 CCGCGG
FauI 136 GCGGG CCCGC(4/6)
518 GCGGG
FokI 70 CATCC GGATG(9/13)
132 GGATG
HgaI 91 GACGC GACGC(5/10)
493 GACGC
HinfI 179 GAGTC G^A[ACGT]TC
286 GATTC
KasI 157 GGCGCC G^GCGCC
634 GGCGCC
MaeIII 51 GTCAC ^GT[ACGT]AC
293 GTGAC
MboI 108 GATC ^GATC
456 GATC
MboII 176 GAAGA GAAGA(8/7)
602 TCTTC
MflI 107 AGATCC [GA]^GATC[CT]
455 AGATCT
MslI 335 CATGTGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
771 CACCACCATG
NaeI 78 GCCGGC GCC^GGC
586 GCCGGC
NarI 157 GGCGCC GG^CGCC
634 GGCGCC
NdeII 108 GATC ^GATC
456 GATC
NgoMIV 78 GCCGGC G^CCGGC
586 GCCGGC
RsaI 645 GTAC GT^AC
780 GTAC
Sau3AI 108 GATC ^GATC
456 GATC
SfaNI 133 GATGC GCATC(5/9)
611 GCATC
SfoI 157 GGCGCC GGC^GCC
634 GGCGCC
Tsp45I 51 GTCAC ^GT[GC]AC
293 GTGAC
TspRI 53 CACTG CA[GC]TG[ACGT][ACGT]^
231 CAGTG
XcmI 362 CCATCGGGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
554 CCAATGGCCTGATGG
XhoII 107 AGATCC [GA]^GATC[CT]
455 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AcyI AluI AvaII
BbvI BmyI BsaHI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI EcoRII Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII HphI ItaI MnlI
MspA1I MspI MvaI MvnI MwoI
NlaIII NlaIV PvuII Sau96I ScrFI
TseI