Restriction map for
pFN21AE5421
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BanI BanII
BbeI BbvCI BbvI BcgI BciVI
BclI BcnI BfrI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaWI BseAI BsgI BsiHKAI
BsiWI BsmI BsoBI Bsp1286I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI Fnu4HI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI ItaI KasI KpnI
Ksp632I KspI MfeI MluI MroI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SwaI TaqI TseI
Tsp45I Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 763 GTCTAC GT^[AC][GT]AC
AocI 870 CCTGAGG CC^T[ACGT]AGG
BalI 368 TGGCCA TGG^CCA
BamHI 150 GGATCC G^GATCC
BbrPI 145 CACGTG CAC^GTG
BfaI 509 CTAG C^TAG
BglI 260 GCCAGAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 145 CACGTG [CT]AC^GT[GA]
BsaBI 210 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 900 GAGGAG GAGGAG(10/8)
BsiEI 357 CGACCG CG[GA][CT]^CG
BsmAI 158 GTCTC GTCTC(1/5)
BsmBI 157 CGTCTC CGTCTC(1/5)
BspMI 503 GCAGGT ACCTGC(4/8)
BsrBI 792 CCGCTC CCGCTC(-3/-3)
Bsu36I 870 CCTGAGG CC^T[ACGT]AGG
Cac8I 188 GCCAGC GC[ACGT]^[ACGT]GC
DrdI 444 GACACTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 870 CCTGAGG CC^T[ACGT]AGG
EcoNI 497 CCTACTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 257 CCGG C^CGG
HindIII 297 AAGCTT A^AGCTT
HpaII 257 CCGG C^CGG
MaeI 509 CTAG C^TAG
MaeII 146 ACGT A^CGT
MaeIII 119 GTAAC ^GT[ACGT]AC
MamI 210 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 368 TGGCCA TGG^CCA
MscI 368 TGGCCA TGG^CCA
MseI 730 TTAA T^TAA
MspA1I 800 CAGCTG C[AC]G^C[GT]G
MspI 257 CCGG C^CGG
MwoI 260 GCCAGAAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 676 CATATG CA^TATG
PleI 656 GAGTC GAGTC(4/5)
PmaCI 145 CACGTG CAC^GTG
PmlI 145 CACGTG CAC^GTG
PstI 501 CTGCAG CTGCA^G
PvuII 800 CAGCTG CAG^CTG
SspI 69 AATATT AAT^ATT
StuI 704 AGGCCT AGG^CCT
Tru9I 730 TTAA T^TAA
TspRI 446 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 455 CCATCAACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AciI 399 CCGC CCGC(-3/-1)
792 CCGC
AvaII 204 GGACC G^G[AT]CC
452 GGTCC
BbsI 805 GAAGAC GAAGAC(2/6)
823 GTCTTC
BpuAI 805 GAAGAC GAAGAC(2/6)
823 GTCTTC
BsmFI 229 GTCCC GGGAC(10/14)
538 GTCCC
Bsp1407I 212 TGTACA T^GTACA
562 TGTACA
BsrGI 212 TGTACA T^GTACA
562 TGTACA
BsrI 64 ACTGG ACTGG(1/-1)
447 ACTGG
BstYI 150 GGATCC [GA]^GATC[CT]
812 AGATCC
DdeI 184 CTCAG C^T[ACGT]AG
871 CTGAG
DsaI 314 CCATGG C^C[GA][CT]GG
347 CCATGG
MflI 150 GGATCC [GA]^GATC[CT]
812 AGATCC
MslI 141 CATCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
165 CACACCGATG
NcoI 314 CCATGG C^CATGG
347 CCATGG
NlaIV 150 GGATCC GG[ACGT]^[ACGT]CC
451 GGGTCC
SfaNI 269 GCATC GCATC(5/9)
635 GCATC
SspBI 212 TGTACA T^GTACA
562 TGTACA
XhoII 150 GGATCC [GA]^GATC[CT]
812 AGATCC
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AfaI AluI AlwI BsaJI BsiYI
BslI BssKI BstF5I BstNI Cfr13I
DpnII EaeI EcoRII EcoT14I FokI
HaeIII HinfI HphI MboI MboII
MnlI MvaI NdeII NlaIII RsaI
Sau3AI Sau96I ScrFI SfcI StyI
TfiI