Restriction map for
pFN21AE2781
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# NO CUT ENZYMES ( 175 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
AlwI AlwNI AocI ApaI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiWI BsmAI
BsmBI BsmI BsoBI BspDI BspEI
BspLU11I BsrBI BsrDI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstYI BstZ17I Bsu36I
CelII Cfr13I ClaI CpoI DdeI
DpnII DraI DraII DrdI Eam1105I
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FokI
FseI FspI HgaI Hin1I HincII
HindII HindIII HinfI HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MboI MfeI MflI MluI MroI
MseI MslI MspA1I MunI NaeI
NarI NdeI NdeII NgoMIV NheI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
Sau3AI Sau96I SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI SwaI TfiI Tru9I
Tsp509I TspRI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AflIII 122 ACGTGT A^C[GA][CT]GT
Alw44I 145 GTGCAC G^TGCAC
Aor51HI 257 AGCGCT AGC^GCT
ApaLI 145 GTGCAC G^TGCAC
Asp700I 189 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 145 GTGCAC G[AT]GC[AT]^C
BalI 320 TGGCCA TGG^CCA
BanI 343 GGCACC G^G[CT][GA]CC
BcnI 310 CCCGG CC^[GC]GG
BglI 223 GCCAGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 121 TACGTG [CT]AC^GT[GA]
BsgI 283 GTGCAG GTGCAG(16/14)
BsiHKAI 145 GTGCAC G[AT]GC[AT]^C
BsmFI 273 GTCCC GGGAC(10/14)
Bsp1407I 125 TGTACA T^GTACA
BspHI 185 TCATGA T^CATGA
BspMI 141 GCAGGT ACCTGC(4/8)
BsrFI 157 ACCGGC [GA]^CCGG[CT]
BsrGI 125 TGTACA T^GTACA
BstNI 240 CCTGG CC^[AT]GG
BstXI 170 CCAAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 157 ACCGGC [GA]^CCGG[CT]
DraIII 328 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 176 CCATGG C^C[GA][CT]GG
Eco47III 257 AGCGCT AGC^GCT
EcoRII 240 CCTGG ^CC[AT]GG
HaeII 257 AGCGCT [GA]GCGC^[CT]
MluNI 320 TGGCCA TGG^CCA
MscI 320 TGGCCA TGG^CCA
MvaI 240 CCTGG CC^[AT]GG
NciI 310 CCCGG CC^[GC]GG
NcoI 176 CCATGG C^CATGG
NotI 95 GCGGCCGC GC^GGCCGC
RcaI 185 TCATGA T^CATGA
SspBI 125 TGTACA T^GTACA
StuI 230 AGGCCT AGG^CCT
TaqI 212 TCGA T^CGA
TthHB8I 212 TCGA T^CGA
XmnI 189 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 94 CGCG CG^CG
277 CGCG
AfaI 126 GTAC GT^AC
363 GTAC
BbvI 303 GCTGC GCAGC(8/12)
306 GCTGC
BmyI 145 GTGCAC G[AGT]GC[ACT]^C
342 GGGCAC
BsiEI 96 CGGCCG CG[GA][CT]^CG
290 CGGCCG
BsiYI 31 CCCAAGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
151 CCCGACACCGG
BslI 31 CCCAAGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
151 CCCGACACCGG
Bsp1286I 145 GTGCAC G[AGT]GC[ACT]^C
342 GGGCAC
BssKI 240 CCTGG ^CC[ACGT]GG
310 CCCGG
BstUI 94 CGCG CG^CG
277 CGCG
EagI 96 CGGCCG C^GGCCG
290 CGGCCG
EclXI 96 CGGCCG C^GGCCG
290 CGGCCG
Eco52I 96 CGGCCG C^GGCCG
290 CGGCCG
FauI 236 CCCGC CCCGC(4/6)
275 CCCGC
HapII 158 CCGG C^CGG
311 CCGG
HpaII 158 CCGG C^CGG
311 CCGG
HphI 56 TCACC GGTGA(8/7)
356 TCACC
MaeII 122 ACGT A^CGT
271 ACGT
MaeIII 55 GTCAC ^GT[ACGT]AC
355 GTCAC
MboII 69 GAAGA GAAGA(8/7)
209 TCTTC
MspI 158 CCGG C^CGG
311 CCGG
MvnI 94 CGCG CG^CG
277 CGCG
NlaIII 177 CATG CATG^
186 CATG
NlaIV 21 GGCTCC GG[ACGT]^[ACGT]CC
343 GGCACC
RsaI 126 GTAC GT^AC
363 GTAC
ScrFI 240 CCTGG CC^[ACGT]GG
310 CCCGG
TseI 303 GCTGC G^C[AT]GC
306 GCTGC
Tsp45I 55 GTCAC ^GT[GC]AC
355 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AciI AluI BsaJI Cac8I CfoI
EaeI EcoT14I Fnu4HI HaeIII HhaI
HinP1I ItaI MnlI MwoI SfaNI
StyI