Restriction map for
pFN21AE0994
##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BamHI
BbeI BbrPI BciVI BfaI BfrI
BlnI BsaBI BsaI BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI Eam1105I
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FseI HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MspA1I MunI NarI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SwaI Tru9I
Tsp509I Tth111I Van91I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AcyI 1669 GACGCC G[GA]^CG[CT]C
AfaI 1080 GTAC GT^AC
AflIII 1320 ACATGT A^C[GA][CT]GT
Alw44I 1030 GTGCAC G^TGCAC
ApaLI 1030 GTGCAC G^TGCAC
AviII 791 TGCGCA TGC^GCA
BbsI 1666 GAAGAC GAAGAC(2/6)
BcgI 768 CGAGGCCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 107 TGATCA T^GATCA
BpmI 1210 CTGGAG CTGGAG(16/14)
BpuAI 1666 GAAGAC GAAGAC(2/6)
BsaAI 355 TACGTG [CT]AC^GT[GA]
BsaHI 1669 GACGCC G[GA]^CG[CT]C
BsmBI 1792 CGTCTC CGTCTC(1/5)
BspLU11I 1320 ACATGT A^CATGT
DraIII 1501 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I 464 CCATGG C^C[AT][AT]GG
FbaI 107 TGATCA T^GATCA
FspI 791 TGCGCA TGC^GCA
HaeII 306 AGCGCC [GA]GCGC^[CT]
Hin1I 1669 GACGCC G[GA]^CG[CT]C
HincII 1114 GTCAAC GT[CT]^[GA]AC
HindII 1114 GTCAAC GT[CT]^[GA]AC
NaeI 635 GCCGGC GCC^GGC
NcoI 464 CCATGG C^CATGG
NgoMIV 635 GCCGGC G^CCGGC
NotI 1052 GCGGCCGC GC^GGCCGC
PleI 451 GACTC GAGTC(4/5)
RsaI 1080 GTAC GT^AC
SacI 1306 GAGCTC GAGCT^C
SapI 702 GAAGAGC GCTCTTC(1/4)
SmaI 602 CCCGGG CCC^GGG
SmlI 823 CTCAAG C^T[CT][GA]AG
SspI 197 AATATT AAT^ATT
StyI 464 CCATGG C^C[AT][AT]GG
XmaCI 602 CCCGGG C^CCGGG
XmaI 602 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 344 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
835 CAGATGCTG
AocI 725 CCTCAGG CC^T[ACGT]AGG
1425 CCTGAGG
BanI 615 GGTGCC G^G[CT][GA]CC
1787 GGCACC
BbvCI 1191 GCTGAGG CCTCAGC(-5/-2)
1782 GCTGAGG
BglI 352 GCCTACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
541 GCCCGGCAGGC
BglII 884 AGATCT A^GATCT
1397 AGATCT
BsgI 1825 CTGCAC GTGCAG(16/14)
172 GTGCAG
BsiEI 62 CGGCCG CG[GA][CT]^CG
1053 CGGCCG
BsrFI 635 GCCGGC [GA]^CCGG[CT]
1642 ACCGGC
BstAPI 794 GCACGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1913 GCAACTACTGC
Bsu36I 725 CCTCAGG CC^T[ACGT]AGG
1425 CCTGAGG
Cfr10I 635 GCCGGC [GA]^CCGG[CT]
1642 ACCGGC
EagI 62 CGGCCG C^GGCCG
1053 CGGCCG
EarI 279 CTCTTC CTCTTC(1/4)
702 GAAGAG
EclXI 62 CGGCCG C^GGCCG
1053 CGGCCG
Eco52I 62 CGGCCG C^GGCCG
1053 CGGCCG
Eco81I 725 CCTCAGG CC^T[ACGT]AGG
1425 CCTGAGG
HgaI 585 GACGC GACGC(5/10)
1669 GACGC
HphI 1553 TCACC GGTGA(8/7)
1640 TCACC
Ksp632I 279 CTCTTC CTCTTC(1/4)
702 GAAGAG
MaeII 356 ACGT A^CGT
1187 ACGT
MaeIII 622 GTGAC ^GT[ACGT]AC
1217 GTGAC
PpuMI 719 AGGACCC [GA]G^G[AT]CC[CT]
1256 GGGACCT
SphI 252 GCATGC GCATG^C
597 GCATGC
TfiI 1386 GATTC G^A[AT]TC
1770 GATTC
Tsp45I 622 GTGAC ^GT[GC]AC
1217 GTGAC
XcmI 498 CCAATCCCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1685 CCATCCTCATCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AluI AlwI ApaI
AspHI AvaI AvaII BalI BanII
BbvI BcnI BlpI BmyI Bpu1102I
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MboI MboII MflI MluNI MnlI
MscI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI TaqI TseI
TspRI TthHB8I XhoII