Restriction map for
pFN21AE0400
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BpuAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrDI BssHII Bst1107I BstBI
BstXI BstYI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MaeI
MamI MflI MluI MroI MseI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
SspI SwaI TfiI Tru9I Tth111I
XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 45 enzymes) #
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AocI 723 CCTCAGG CC^T[ACGT]AGG
Asp700I 525 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 737 GAGCTC G[AT]GC[AT]^C
BalI 922 TGGCCA TGG^CCA
BbrPI 166 CACGTG CAC^GTG
BsaAI 166 CACGTG [CT]AC^GT[GA]
BseRI 70 GAGGAG GAGGAG(10/8)
BsiHKAI 737 GAGCTC G[AT]GC[AT]^C
BsmAI 958 GAGAC GTCTC(1/5)
Bsp1407I 803 TGTACA T^GTACA
BsrGI 803 TGTACA T^GTACA
BstEII 432 GGTGACC G^GT[ACGT]ACC
BstPI 432 GGTGACC G^GT[ACGT]ACC
Bsu36I 723 CCTCAGG CC^T[ACGT]AGG
DpnII 978 GATC ^GATC
EarI 358 CTCTTC CTCTTC(1/4)
Eco81I 723 CCTCAGG CC^T[ACGT]AGG
EcoNI 63 CCTGACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 432 GGTGACC G^GT[ACGT]ACC
HinfI 490 GACTC G^A[ACGT]TC
HphI 432 GGTGA GGTGA(8/7)
Ksp632I 358 CTCTTC CTCTTC(1/4)
KspI 219 CCGCGG CCGC^GG
MboI 978 GATC ^GATC
MboII 359 TCTTC GAAGA(8/7)
MfeI 918 CAATTG C^AATTG
MluNI 922 TGGCCA TGG^CCA
MscI 922 TGGCCA TGG^CCA
MslI 1035 CATGAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 918 CAATTG C^AATTG
NdeII 978 GATC ^GATC
PleI 490 GACTC GAGTC(4/5)
PmaCI 166 CACGTG CAC^GTG
PmlI 166 CACGTG CAC^GTG
SacI 737 GAGCTC GAGCT^C
SacII 219 CCGCGG CCGC^GG
SapI 357 GCTCTTC GCTCTTC(1/4)
Sau3AI 978 GATC ^GATC
SbfI 813 CCTGCAGG CCTGCA^GG
Sse8387I 813 CCTGCAGG CCTGCA^GG
SspBI 803 TGTACA T^GTACA
StuI 527 AGGCCT AGG^CCT
Tsp509I 919 AATT ^AATT
TspRI 943 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 525 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 124 GTAC GT^AC
804 GTAC
ApaI 173 GGGCCC GGGCC^C
624 GGGCCC
BbeI 640 GGCGCC GGCGC^C
675 GGCGCC
BlpI 108 GCTCAGC GC^T[ACGT]AGC
567 GCTGAGC
Bpu1102I 108 GCTCAGC GC^T[ACGT]AGC
567 GCTGAGC
BsrBI 213 CCGCTC CCGCTC(-3/-3)
516 GAGCGG
BssSI 511 CACGAG CACGAG(-5/-1)
1075 CTCGTG
CelII 108 GCTCAGC GC^T[ACGT]AGC
567 GCTGAGC
DsaI 204 CCACGG C^C[GA][CT]GG
219 CCGCGG
EaeI 254 TGGCCG [CT]^GGCC[GA]
922 TGGCCA
EcoT14I 377 CCAAGG C^C[AT][AT]GG
1081 CCAAGG
KasI 640 GGCGCC G^GCGCC
675 GGCGCC
MaeII 167 ACGT A^CGT
536 ACGT
NaeI 256 GCCGGC GCC^GGC
572 GCCGGC
NarI 640 GGCGCC GG^CGCC
675 GGCGCC
NgoMIV 256 GCCGGC G^CCGGC
572 GCCGGC
PflMI 645 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
833 CCAAGAACTGG
PstI 103 CTGCAG CTGCA^G
814 CTGCAG
RsaI 124 GTAC GT^AC
804 GTAC
SfaNI 618 GCATC GCATC(5/9)
986 GCATC
SfoI 640 GGCGCC GGC^GCC
675 GGCGCC
StyI 377 CCAAGG C^C[AT][AT]GG
1081 CCAAGG
Van91I 645 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
833 CCAAGAACTGG
XcmI 326 CCAACAGCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1007 CCAGGCCCCAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AvaI AvaII BanI BanII BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BsgI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII ItaI
MaeIII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PvuII Sau96I ScrFI SfcI SmaI
TaqI TseI Tsp45I TthHB8I XmaCI
XmaI