##################################
# NO CUT ENZYMES (  205 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      Cfr13I      ClaI        CpoI        
DpnII       DraI        DraII       DraIII      DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FokI        FseI        FspI        HaeII       HaeIII      
HapII       HgaI        Hin1I       HinfI       HpaI        
HpaII       HphI        KasI        KpnI        Ksp632I     
KspI        MaeI        MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspA1I      MspI        MunI        NaeI        NarI        
NciI        NdeI        NdeII       NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      Sau96I      SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        TfiI        Tsp45I      TspRI       
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccII          34  CGCG                 CG^CG
AcsI          190  AAATTC               [GA]^AATT[CT]
AfaI            9  GTAC                 GT^AC
AflIII         75  ACGTGT               A^C[GA][CT]GT
ApoI          190  AAATTC               [GA]^AATT[CT]
BbrPI          74  CACGTG               CAC^GTG
BbvI           37  GCTGC                GCAGC(8/12)
BsaAI          74  CACGTG               [CT]AC^GT[GA]
BseRI          22  GAGGAG               GAGGAG(10/8)
BsmI           96  GCATTC               GAATGC(1/-1)
BsrDI         236  GCAATG               GCAATG(2/0)
BssKI          17  CCTGG                ^CC[ACGT]GG
BstNI          17  CCTGG                CC^[AT]GG
BstUI          34  CGCG                 CG^CG
Cac8I         232  GCCCGC               GC[ACGT]^[ACGT]GC
CfoI           35  GCGC                 GCG^C
DdeI          254  CTGAG                C^T[ACGT]AG
DsaI          245  CCATGG               C^C[GA][CT]GG
EcoRII         17  CCTGG                ^CC[AT]GG
FauI          233  CCCGC                CCCGC(4/6)
HhaI           35  GCGC                 GCG^C
HinP1I         35  GCGC                 G^CGC
HincII        200  GTCAAC               GT[CT]^[GA]AC
HindII        200  GTCAAC               GT[CT]^[GA]AC
HindIII        46  AAGCTT               A^AGCTT
MaeII          75  ACGT                 A^CGT
MboII         180  GAAGA                GAAGA(8/7)
MseI          119  TTAA                 T^TAA
MslI           97  CATTCTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI           17  CCTGG                CC^[AT]GG
MvnI           34  CGCG                 CG^CG
MwoI          223  GCCAAGGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          245  CCATGG               C^CATGG
NlaIII        246  CATG                 CATG^
PmaCI          74  CACGTG               CAC^GTG
PmlI           74  CACGTG               CAC^GTG
RsaI            9  GTAC                 GT^AC
ScrFI          17  CCTGG                CC^[ACGT]GG
SmlI          167  CTCAAG               C^T[CT][GA]AG
Tru9I         119  TTAA                 T^TAA
TseI           37  GCTGC                G^C[AT]GC
Tsp509I       191  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AluI           47  AGCT                 AG^CT
              165  AGCT                
BsiYI          12  CCAGACCTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               63  CCCTATGAAGG         
BslI           12  CCAGACCTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               63  CCCTATGAAGG         
EcoT14I       224  CCAAGG               C^C[AT][AT]GG
              245  CCATGG              
MaeIII        124  GTAAC                ^GT[ACGT]AC
              241  GTTAC               
MnlI           54  CCTC                 CCTC(7/6)
               22  GAGG                
StyI          224  CCAAGG               C^C[AT][AT]GG
              245  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

AciI        BsaJI       Fnu4HI      ItaI