Restriction map for
pFN21AE1749
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# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BanII BbeI BbrPI BbvCI
BciVI BclI BcnI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TaqI
Tth111I TthHB8I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AciI 228 CCGC CCGC(-3/-1)
AfaI 504 GTAC GT^AC
Alw44I 557 GTGCAC G^TGCAC
AocI 467 CCTTAGG CC^T[ACGT]AGG
ApaLI 557 GTGCAC G^TGCAC
AvaII 369 GGACC G^G[AT]CC
BamHI 472 GGATCC G^GATCC
BanI 381 GGCACC G^G[CT][GA]CC
BcgI 95 GCATATTTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 364 CTAG C^TAG
BglI 227 GCCGCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 700 GAGGAG GAGGAG(10/8)
BsgI 520 GTGCAG GTGCAG(16/14)
BstAPI 613 GCACTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 333 CATCC GGATG(2/0)
BstYI 472 GGATCC [GA]^GATC[CT]
Bsu36I 467 CCTTAGG CC^T[ACGT]AGG
DraII 282 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 653 CCATGG C^C[GA][CT]GG
Eco81I 467 CCTTAGG CC^T[ACGT]AGG
EcoO109I 282 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 653 CCATGG C^C[AT][AT]GG
FokI 333 CATCC GGATG(9/13)
MaeI 364 CTAG C^TAG
MflI 472 GGATCC [GA]^GATC[CT]
MseI 676 TTAA T^TAA
NcoI 653 CCATGG C^CATGG
PflMI 433 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 504 GTAC GT^AC
SexAI 161 ACCTGGT A^CC[AT]GGT
SfaNI 55 GATGC GCATC(5/9)
SmlI 337 CTCAAG C^T[CT][GA]AG
SspI 710 AATATT AAT^ATT
StyI 653 CCATGG C^C[AT][AT]GG
Tru9I 676 TTAA T^TAA
Van91I 433 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 363 TCTAGA T^CTAGA
XhoII 472 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 124 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
281 CAGGGCCTG
AspHI 61 GAGCAC G[AT]GC[AT]^C
557 GTGCAC
BbsI 174 GAAGAC GAAGAC(2/6)
496 GAAGAC
BbvI 344 GCAGC GCAGC(8/12)
528 GCAGC
BpmI 80 CTCCAG CTGGAG(16/14)
718 CTGGAG
BpuAI 174 GAAGAC GAAGAC(2/6)
496 GAAGAC
BsiHKAI 61 GAGCAC G[AT]GC[AT]^C
557 GTGCAC
BsiYI 372 CCACACCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
433 CCAGTTCATGG
BslI 372 CCACACCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
433 CCAGTTCATGG
BsmFI 133 GGGAC GGGAC(10/14)
410 GGGAC
Cac8I 16 GCGTGC GC[ACGT]^[ACGT]GC
518 GCGTGC
HaeIII 235 GGCC GG^CC
284 GGCC
HinfI 126 GATTC G^A[ACGT]TC
429 GAATC
HphI 258 GGTGA GGTGA(8/7)
464 TCACC
MaeII 140 ACGT A^CGT
310 ACGT
MaeIII 299 GTGAC ^GT[ACGT]AC
416 GTGAC
MslI 309 CACGTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
690 CATGACAATG
TfiI 126 GATTC G^A[AT]TC
429 GAATC
TseI 344 GCAGC G^C[AT]GC
528 GCAGC
Tsp45I 299 GTGAC ^GT[GC]AC
416 GTGAC
Tsp509I 674 AATT ^AATT
749 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AluI AlwI BmyI BsaJI Bsp1286I
BsrDI BsrI BssKI BstNI Cfr13I
DdeI DpnII EcoRII Fnu4HI ItaI
MboI MboII MnlI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI TspRI