Restriction map for
pFN21AE1659
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccI AccII AciI
AclI AcsI AcyI AfaI AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaJI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssKI BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstUI BstXI BstYI BstZ17I
CelII Cfr10I CpoI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRII EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HaeIII HgaI Hin1I HincII HindII
HindIII HpaI HphI KasI KpnI
Ksp632I KspI MaeII MamI MfeI
MflI MluI MluNI MscI MslI
MspA1I MunI MvaI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI ScrFI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 317 TCCGGA T^CCGGA
AflII 192 CTTAAG C^TTAAG
AlwNI 430 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 460 CCTCAGG CC^T[ACGT]AGG
AspI 201 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 363 GGACC G^G[AT]CC
BbsI 75 GAAGAC GAAGAC(2/6)
BfrI 192 CTTAAG C^TTAAG
BpuAI 75 GAAGAC GAAGAC(2/6)
BsaWI 317 TCCGGA [AT]^CCGG[AT]
BseAI 317 TCCGGA T^CCGGA
BseRI 517 GAGGAG GAGGAG(10/8)
BsmAI 520 GAGAC GTCTC(1/5)
BspDI 233 ATCGAT AT^CGAT
BspEI 317 TCCGGA T^CCGGA
BsrI 67 ACTGG ACTGG(1/-1)
BstF5I 512 GGATG GGATG(2/0)
Bsu36I 460 CCTCAGG CC^T[ACGT]AGG
CfoI 249 GCGC GCG^C
Cfr13I 363 GGACC G^G[ACGT]CC
ClaI 233 ATCGAT AT^CGAT
DraI 283 TTTAAA TTT^AAA
DraII 362 AGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 460 CCTCAGG CC^T[ACGT]AGG
EcoO109I 362 AGGACCT [GA]G^G[ACGT]CC[CT]
FokI 512 GGATG GGATG(9/13)
HhaI 249 GCGC GCG^C
HinP1I 249 GCGC G^CGC
MaeIII 440 GTCAC ^GT[ACGT]AC
MroI 317 TCCGGA T^CCGGA
NlaIV 456 GGAACC GG[ACGT]^[ACGT]CC
NspI 391 ACATGC [GA]CATG^[CT]
PflFI 201 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 362 AGGACCT [GA]G^G[AT]CC[CT]
Sau96I 363 GGACC G^G[ACGT]CC
SfaNI 236 GATGC GCATC(5/9)
SmlI 192 CTTAAG C^T[CT][GA]AG
TaqI 234 TCGA T^CGA
Tsp45I 440 GTCAC ^GT[GC]AC
Tth111I 201 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 234 TCGA T^CGA
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AluI 94 AGCT AG^CT
452 AGCT
BfaI 11 CTAG C^TAG
493 CTAG
BsiYI 61 CCTTCCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
396 CCATTGTGGGG
BslI 61 CCTTCCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
396 CCATTGTGGGG
BsrDI 412 GCAATG GCAATG(2/0)
507 GCAATG
Cac8I 159 GCAAGC GC[ACGT]^[ACGT]GC
225 GCCTGC
DpnII 102 GATC ^GATC
324 GATC
HapII 318 CCGG C^CGG
369 CCGG
HpaII 318 CCGG C^CGG
369 CCGG
MaeI 11 CTAG C^TAG
493 CTAG
MboI 102 GATC ^GATC
324 GATC
MseI 193 TTAA T^TAA
284 TTAA
MspI 318 CCGG C^CGG
369 CCGG
MwoI 242 GCCTCCTGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
249 GCGCCATTTGC
NdeII 102 GATC ^GATC
324 GATC
Sau3AI 102 GATC ^GATC
324 GATC
Tru9I 193 TTAA T^TAA
284 TTAA
Tsp509I 85 AATT ^AATT
340 AATT
TspRI 66 CACTG CA[GC]TG[ACGT][ACGT]^
153 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
BbvI DdeI Fnu4HI HinfI ItaI
MboII MnlI NlaIII PleI TfiI
TseI