Restriction map for
pFN21AE3758
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BbeI BbrPI BcgI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmFI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CelII CfoI ClaI CpoI
DraI DraII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MamI MluI MluNI MroI
MscI MspA1I MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AciI 411 CCGC CCGC(-3/-1)
AgeI 597 ACCGGT A^CCGGT
AlwNI 37 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 541 CCTCAGG CC^T[ACGT]AGG
Asp700I 797 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 279 GGATCC G^GATCC
BbsI 1287 GAAGAC GAAGAC(2/6)
BbvCI 385 CCTCAGC CCTCAGC(-5/-2)
BciVI 1118 GTATCC GTATCC(6/5)
BclI 995 TGATCA T^GATCA
BpuAI 1287 GAAGAC GAAGAC(2/6)
BsaWI 597 ACCGGT [AT]^CCGG[AT]
BsmBI 127 CGTCTC CGTCTC(1/5)
Bsp1407I 242 TGTACA T^GTACA
BsrDI 413 GCAATG GCAATG(2/0)
BsrFI 597 ACCGGT [GA]^CCGG[CT]
BsrGI 242 TGTACA T^GTACA
BssSI 999 CACGAG CACGAG(-5/-1)
BstF5I 1245 GGATG GGATG(2/0)
BstXI 350 CCAAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 541 CCTCAGG CC^T[ACGT]AGG
Cac8I 108 GCAGGC GC[ACGT]^[ACGT]GC
Cfr10I 597 ACCGGT [GA]^CCGG[CT]
DraIII 15 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 115 GAAGAG CTCTTC(1/4)
Eco81I 541 CCTCAGG CC^T[ACGT]AGG
FbaI 995 TGATCA T^GATCA
FokI 1245 GGATG GGATG(9/13)
HapII 598 CCGG C^CGG
HpaII 598 CCGG C^CGG
Ksp632I 115 GAAGAG CTCTTC(1/4)
MaeII 731 ACGT A^CGT
MfeI 626 CAATTG C^AATTG
MseI 98 TTAA T^TAA
MslI 987 CACCCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 598 CCGG C^CGG
MunI 626 CAATTG C^AATTG
MwoI 93 GCAGTTTAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 666 CCAAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 597 ACCGGT A^CCGGT
PleI 1095 GACTC GAGTC(4/5)
PstI 43 CTGCAG CTGCA^G
ScaI 1132 AGTACT AGT^ACT
SspBI 242 TGTACA T^GTACA
TaqI 833 TCGA T^CGA
Tru9I 98 TTAA T^TAA
TthHB8I 833 TCGA T^CGA
Van91I 666 CCAAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 797 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AcsI 568 AAATTT [GA]^AATT[CT]
882 GAATTT
ApoI 568 AAATTT [GA]^AATT[CT]
882 GAATTT
AvaII 77 GGACC G^G[AT]CC
1249 GGACC
BanI 394 GGTGCC G^G[CT][GA]CC
981 GGCACC
BbvI 341 GCAGC GCAGC(8/12)
613 GCTGC
BfaI 407 CTAG C^TAG
922 CTAG
BsaJI 704 CCCTGG C^C[ACGT][ACGT]GG
1260 CCCTGG
BseRI 468 CTCCTC GAGGAG(10/8)
539 CTCCTC
BstYI 279 GGATCC [GA]^GATC[CT]
952 AGATCC
MaeI 407 CTAG C^TAG
922 CTAG
MflI 279 GGATCC [GA]^GATC[CT]
952 AGATCC
TseI 341 GCAGC G^C[AT]GC
613 GCTGC
XhoII 279 GGATCC [GA]^GATC[CT]
952 AGATCC
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AfaI AluI AlwI BanII BmyI
BpmI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstNI Cfr13I DdeI
DpnII EcoRII Fnu4HI HaeIII HindIII
HinfI HphI ItaI MaeIII MboI
MboII MnlI MvaI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfcI TfiI Tsp45I Tsp509I TspRI