Restriction map for
pFN21AE9461
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbsI BcgI BclI BfaI BfrI
BglII BlnI BpuAI BsaBI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstUI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeI MamI MfeI
MluI MroI MseI MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 1099 GTCTAC GT^[AC][GT]AC
AcyI 1225 GGCGCC G[GA]^CG[CT]C
AocI 181 CCTGAGG CC^T[ACGT]AGG
AvaI 883 CCCGAG C^[CT]CG[GA]G
BbeI 1225 GGCGCC GGCGC^C
BbrPI 688 CACGTG CAC^GTG
BbvCI 869 CCTCAGC CCTCAGC(-5/-2)
BciVI 360 GTATCC GTATCC(6/5)
BcnI 203 CCCGG CC^[GC]GG
BlpI 54 GCTAAGC GC^T[ACGT]AGC
BpmI 1013 CTCCAG CTGGAG(16/14)
Bpu1102I 54 GCTAAGC GC^T[ACGT]AGC
BsaAI 688 CACGTG [CT]AC^GT[GA]
BsaHI 1225 GGCGCC G[GA]^CG[CT]C
BsaWI 363 TCCGGT [AT]^CCGG[AT]
BsmFI 888 GTCCC GGGAC(10/14)
BsoBI 883 CCCGAG C^[CT]CG[GA]G
BspHI 860 TCATGA T^CATGA
BspMI 979 ACCTGC ACCTGC(4/8)
BsrBI 727 GAGCGG CCGCTC(-3/-3)
BssSI 1048 CACGAG CACGAG(-5/-1)
BstEII 366 GGTGACC G^GT[ACGT]ACC
BstPI 366 GGTGACC G^GT[ACGT]ACC
BstYI 344 AGATCC [GA]^GATC[CT]
Bsu36I 181 CCTGAGG CC^T[ACGT]AGG
CelII 54 GCTAAGC GC^T[ACGT]AGC
DraII 1008 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 1368 GACCGAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 181 CCTGAGG CC^T[ACGT]AGG
EcoNI 705 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1008 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 366 GGTGACC G^GT[ACGT]ACC
EcoT22I 855 ATGCAT ATGCA^T
HgaI 780 GACGC GACGC(5/10)
Hin1I 1225 GGCGCC G[GA]^CG[CT]C
KasI 1225 GGCGCC G^GCGCC
MflI 344 AGATCC [GA]^GATC[CT]
NarI 1225 GGCGCC GG^CGCC
NciI 203 CCCGG CC^[GC]GG
NcoI 836 CCATGG C^CATGG
NsiI 855 ATGCAT ATGCA^T
NspI 926 GCATGT [GA]CATG^[CT]
PmaCI 688 CACGTG CAC^GTG
PmlI 688 CACGTG CAC^GTG
PshAI 1092 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 860 TCATGA T^CATGA
SapI 483 GAAGAGC GCTCTTC(1/4)
SfoI 1225 GGCGCC GGC^GCC
SmlI 1363 CTCAAG C^T[CT][GA]AG
TaqI 725 TCGA T^CGA
TthHB8I 725 TCGA T^CGA
XhoII 344 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 198 GTAC GT^AC
936 GTAC
AlwI 128 GATCC GGATC(4/5)
345 GATCC
AspHI 375 GAGCTC G[AT]GC[AT]^C
796 GAGCTC
AvaII 534 GGTCC G^G[AT]CC
1182 GGACC
BglI 508 GCCAGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
748 GCCAAGTTGGC
BsiHKAI 375 GAGCTC G[AT]GC[AT]^C
796 GAGCTC
BstAPI 171 GCAGTATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1433 GCACACCTTGC
BstXI 236 CCAAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
776 CCATGACGCTGG
HapII 204 CCGG C^CGG
364 CCGG
HpaII 204 CCGG C^CGG
364 CCGG
MspI 204 CCGG C^CGG
364 CCGG
PflMI 286 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1173 CCAGACCCTGG
PstI 226 CTGCAG CTGCA^G
1257 CTGCAG
PvuII 811 CAGCTG CAG^CTG
985 CAGCTG
RsaI 198 GTAC GT^AC
936 GTAC
SacI 375 GAGCTC GAGCT^C
796 GAGCTC
StuI 1274 AGGCCT AGG^CCT
1331 AGGCCT
Van91I 286 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1173 CCAGACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AluI AlwNI BalI BanI
BanII BbvI BmyI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TseI Tsp45I TspRI
XcmI