Restriction map for
pFN21AE1991
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BclI BcnI
BglI BglII BlnI BpuAI BsaAI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmI BspDI BspEI
BspHI BspMI BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstZ17I Bsu36I Cac8I CfoI Cfr10I
ClaI CpoI DraI DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MfeI MluI MluNI MroI
MscI MspA1I MspI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AciI 1171 CCGC CCGC(-3/-1)
AflII 982 CTTAAG C^TTAAG
ApaI 1348 GGGCCC GGGCC^C
AseI 934 ATTAAT AT^TAAT
AsnI 934 ATTAAT AT^TAAT
Asp700I 254 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 823 CTCGAG C^[CT]CG[GA]G
BbvI 419 GCAGC GCAGC(8/12)
BfrI 982 CTTAAG C^TTAAG
BlpI 472 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 472 GCTCAGC GC^T[ACGT]AGC
BsgI 1140 CTGCAC GTGCAG(16/14)
BsiYI 41 CCACTGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 41 CCACTGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 503 GAGACG CGTCTC(1/5)
BsmFI 850 GTCCC GGGAC(10/14)
BsoBI 823 CTCGAG C^[CT]CG[GA]G
Bsp1407I 327 TGTACA T^GTACA
BspLU11I 802 ACATGT A^CATGT
BsrDI 904 GCAATG GCAATG(2/0)
BsrGI 327 TGTACA T^GTACA
BstAPI 206 GCATAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1512 AGATCC [GA]^GATC[CT]
CelII 472 GCTCAGC GC^T[ACGT]AGC
DraII 1348 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 87 CCATGG C^C[GA][CT]GG
EcoO109I 1348 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1612 GAATTC G^AATTC
EcoRV 1644 GATATC GAT^ATC
EcoT14I 87 CCATGG C^C[AT][AT]GG
Fnu4HI 419 GCAGC GC^[ACGT]GC
HincII 1413 GTCAAC GT[CT]^[GA]AC
HindII 1413 GTCAAC GT[CT]^[GA]AC
ItaI 419 GCAGC GC^[ACGT]GC
MflI 1512 AGATCC [GA]^GATC[CT]
MwoI 206 GCATAACATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 87 CCATGG C^CATGG
NdeI 414 CATATG CA^TATG
PaeR7I 823 CTCGAG C^TCGAG
PshBI 934 ATTAAT AT^TAAT
PstI 522 CTGCAG CTGCA^G
SspBI 327 TGTACA T^GTACA
StyI 87 CCATGG C^C[AT][AT]GG
TseI 419 GCAGC G^C[AT]GC
XbaI 1057 TCTAGA T^CTAGA
XhoI 823 CTCGAG C^TCGAG
XhoII 1512 AGATCC [GA]^GATC[CT]
XmnI 254 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 1135 GTAGAC GT^[AC][GT]AC
1332 GTCTAC
AflIII 506 ACGTGT A^C[GA][CT]GT
802 ACATGT
AlwI 1513 GATCC GGATC(4/5)
547 GGATC
AvaII 401 GGTCC G^G[AT]CC
625 GGTCC
BanII 1348 GGGCCC G[GA]GC[CT]^C
1455 GGGCTC
BfaI 1058 CTAG C^TAG
1510 CTAG
BmyI 1348 GGGCCC G[AGT]GC[ACT]^C
1455 GGGCTC
BpmI 1406 CTCCAG CTGGAG(16/14)
1450 CTCCAG
BsaBI 706 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1304 GATGGCAATC
BseRI 407 CTCCTC GAGGAG(10/8)
632 CTCCTC
Bsp1286I 1348 GGGCCC G[AGT]GC[ACT]^C
1455 GGGCTC
BsrI 1434 CCAGT ACTGG(1/-1)
1617 CCAGT
BssKI 1408 CCAGG ^CC[ACGT]GG
1452 CCAGG
BstEII 230 GGTCACC G^GT[ACGT]ACC
581 GGTCACC
BstNI 1408 CCAGG CC^[AT]GG
1452 CCAGG
BstPI 230 GGTCACC G^GT[ACGT]ACC
581 GGTCACC
DraIII 62 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
1319 CACGCTGTG
EcoO65I 230 GGTCACC G^GT[ACGT]ACC
581 GGTCACC
EcoRII 1408 CCAGG ^CC[AT]GG
1452 CCAGG
HaeIII 734 GGCC GG^CC
1349 GGCC
MaeI 1058 CTAG C^TAG
1510 CTAG
MamI 706 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1304 GATGGCAATC
MslI 239 CACCGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1499 CACCAAGATG
MvaI 1408 CCAGG CC^[AT]GG
1452 CCAGG
NlaIV 1348 GGGCCC GG[ACGT]^[ACGT]CC
1349 GGCCCC
ScrFI 1408 CCAGG CC^[ACGT]GG
1452 CCAGG
SfcI 137 CTACAG C^T[GA][CT]AG
522 CTGCAG
TfiI 292 GAATC G^A[AT]TC
1125 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AfaI AluI ApoI BciVI
BsaJI BsmAI BstF5I Cfr13I DdeI
DpnII FokI HinfI HphI MaeII
MaeIII MboI MboII MnlI MseI
NdeII NlaIII NspI PleI RsaI
Sau3AI Sau96I SfaNI SmlI TaqI
Tru9I Tsp45I Tsp509I TspRI TthHB8I