Restriction map for
pFN21AE2190
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI AocI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvrII BanI BanII
BbeI BbrPI BcgI BciVI BclI
BfrI BglI BlnI BpmI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoT22I FauI FbaI
FseI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MslI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 1282 GTAGAC GT^[AC][GT]AC
AccII 1321 CGCG CG^CG
Aor51HI 331 AGCGCT AGC^GCT
Asp700I 241 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 662 CCCGAG C^[CT]CG[GA]G
AviII 839 TGCGCA TGC^GCA
BalI 1369 TGGCCA TGG^CCA
BbvCI 646 GCTGAGG CCTCAGC(-5/-2)
BcnI 986 CCCGG CC^[GC]GG
BfaI 601 CTAG C^TAG
BlpI 12 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 12 GCTTAGC GC^T[ACGT]AGC
BseRI 630 GAGGAG GAGGAG(10/8)
BsmAI 65 GAGAC GTCTC(1/5)
BsmBI 65 GAGACG CGTCTC(1/5)
BsoBI 662 CCCGAG C^[CT]CG[GA]G
Bsp1407I 492 TGTACA T^GTACA
BspMI 804 ACCTGC ACCTGC(4/8)
BsrGI 492 TGTACA T^GTACA
BstUI 1321 CGCG CG^CG
BstXI 560 CCAAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 786 GCGTGC GC[ACGT]^[ACGT]GC
CelII 12 GCTTAGC GC^T[ACGT]AGC
EaeI 1369 TGGCCA [CT]^GGCC[GA]
EarI 427 GAAGAG CTCTTC(1/4)
Eco47III 331 AGCGCT AGC^GCT
EcoNI 1137 CCTTCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 287 GATATC GAT^ATC
FspI 839 TGCGCA TGC^GCA
HaeII 331 AGCGCT [GA]GCGC^[CT]
HapII 987 CCGG C^CGG
HpaII 987 CCGG C^CGG
Ksp632I 427 GAAGAG CTCTTC(1/4)
MaeI 601 CTAG C^TAG
MluNI 1369 TGGCCA TGG^CCA
MscI 1369 TGGCCA TGG^CCA
MspA1I 860 CAGCTG C[AC]G^C[GT]G
MspI 987 CCGG C^CGG
MvnI 1321 CGCG CG^CG
NciI 986 CCCGG CC^[GC]GG
PvuII 860 CAGCTG CAG^CTG
ScaI 158 AGTACT AGT^ACT
SspBI 492 TGTACA T^GTACA
SspI 244 AATATT AAT^ATT
XbaI 600 TCTAGA T^CTAGA
XmnI 241 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AciI 1320 CCGC CCGC(-3/-1)
4 GCGG
AcsI 729 GAATTT [GA]^AATT[CT]
1153 AAATTT
Alw44I 199 GTGCAC G^TGCAC
788 GTGCAC
ApaLI 199 GTGCAC G^TGCAC
788 GTGCAC
ApoI 729 GAATTT [GA]^AATT[CT]
1153 AAATTT
AspHI 199 GTGCAC G[AT]GC[AT]^C
788 GTGCAC
BamHI 828 GGATCC G^GATCC
1455 GGATCC
BglII 276 AGATCT A^GATCT
580 AGATCT
BsgI 230 GTGCAG GTGCAG(16/14)
1339 GTGCAG
BsiHKAI 199 GTGCAC G[AT]GC[AT]^C
788 GTGCAC
BstNI 92 CCTGG CC^[AT]GG
1240 CCAGG
DraII 1145 AGGACCT [GA]G^G[ACGT]CC[CT]
1344 GGGTCCT
DsaI 945 CCATGG C^C[GA][CT]GG
1372 CCATGG
EcoO109I 1145 AGGACCT [GA]G^G[ACGT]CC[CT]
1344 GGGTCCT
EcoRII 92 CCTGG ^CC[AT]GG
1240 CCAGG
EcoT14I 945 CCATGG C^C[AT][AT]GG
1372 CCATGG
HgaI 17 GCGTC GACGC(5/10)
304 GCGTC
HincII 306 GTCAAC GT[CT]^[GA]AC
1190 GTCAAC
HindII 306 GTCAAC GT[CT]^[GA]AC
1190 GTCAAC
MvaI 92 CCTGG CC^[AT]GG
1240 CCAGG
NcoI 945 CCATGG C^CATGG
1372 CCATGG
PleI 993 GACTC GAGTC(4/5)
999 GACTC
PpuMI 1145 AGGACCT [GA]G^G[AT]CC[CT]
1344 GGGTCCT
StyI 945 CCATGG C^C[AT][AT]GG
1372 CCATGG
TaqI 270 TCGA T^CGA
1071 TCGA
Tsp45I 476 GTCAC ^GT[GC]AC
658 GTGAC
TthHB8I 270 TCGA T^CGA
1071 TCGA
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AfaI AluI AlwI AlwNI AvaII
BbsI BbvI BmyI BpuAI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstYI CfoI Cfr13I
DdeI DpnII Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MseI MwoI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TfiI
Tru9I TseI Tsp509I TspRI XhoII