Restriction map for
pFN21AE3278
##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BfrI BglI BglII BlnI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BstAPI BstEII BstPI Bsu36I ClaI
CpoI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoT22I FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI NarI NcoI NheI
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 1654 GTATAC GT^[AC][GT]AC
AccII 24 CGCG CG^CG
BanII 1258 GAGCTC G[GA]GC[CT]^C
BcgI 1611 CGATAAGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1038 GTATCC GTATCC(6/5)
BclI 1911 TGATCA T^GATCA
BfaI 1924 CTAG C^TAG
BsaBI 804 GATAAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1603 GGTCTC GGTCTC(1/5)
BsgI 683 CTGCAC GTGCAG(16/14)
BspMI 245 GCAGGT ACCTGC(4/8)
BsrDI 1773 CATTGC GCAATG(2/0)
BsrFI 2009 GCCGGC [GA]^CCGG[CT]
Bst1107I 1654 GTATAC GTA^TAC
BstBI 467 TTCGAA TT^CGAA
BstUI 24 CGCG CG^CG
BstXI 1941 CCAGGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1654 GTATAC GTA^TAC
Cfr10I 2009 GCCGGC [GA]^CCGG[CT]
DraI 769 TTTAAA TTT^AAA
EcoRV 1397 GATATC GAT^ATC
EcoT14I 2033 CCAAGG C^C[AT][AT]GG
FbaI 1911 TGATCA T^GATCA
MaeI 1924 CTAG C^TAG
MamI 804 GATAAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 24 CGCG CG^CG
NaeI 2009 GCCGGC GCC^GGC
NgoMIV 2009 GCCGGC G^CCGGC
NspV 467 TTCGAA TT^CGAA
PleI 1917 GACTC GAGTC(4/5)
PshAI 1597 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 433 CTGCAG CTGCA^G
PvuII 1195 CAGCTG CAG^CTG
SacI 1258 GAGCTC GAGCT^C
SfuI 467 TTCGAA TT^CGAA
SphI 997 GCATGC GCATG^C
StyI 2033 CCAAGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AspHI 13 GAGCAC G[AT]GC[AT]^C
1258 GAGCTC
AvaI 37 CCCGAG C^[CT]CG[GA]G
967 CTCGGG
BanI 105 GGCACC G^G[CT][GA]CC
980 GGTGCC
BbsI 2062 GAAGAC GAAGAC(2/6)
340 GTCTTC
BcnI 1109 CCCGG CC^[GC]GG
1641 CCGGG
BlpI 238 GCTCAGC GC^T[ACGT]AGC
676 GCTCAGC
BmyI 13 GAGCAC G[AGT]GC[ACT]^C
1258 GAGCTC
Bpu1102I 238 GCTCAGC GC^T[ACGT]AGC
676 GCTCAGC
BpuAI 2062 GAAGAC GAAGAC(2/6)
340 GTCTTC
BsaJI 966 CCTCGG C^C[ACGT][ACGT]GG
2033 CCAAGG
BsiHKAI 13 GAGCAC G[AT]GC[AT]^C
1258 GAGCTC
BsmI 1617 GAATGC GAATGC(1/-1)
1358 GCATTC
BsoBI 37 CCCGAG C^[CT]CG[GA]G
967 CTCGGG
Bsp1286I 13 GAGCAC G[AGT]GC[ACT]^C
1258 GAGCTC
BssSI 509 CTCGTG CACGAG(-5/-1)
2039 CTCGTG
BstYI 494 AGATCC [GA]^GATC[CT]
1584 GGATCT
CelII 238 GCTCAGC GC^T[ACGT]AGC
676 GCTCAGC
DraII 1280 AGGGCCT [GA]G^G[ACGT]CC[CT]
1414 AGGGCCT
EcoO109I 1280 AGGGCCT [GA]G^G[ACGT]CC[CT]
1414 AGGGCCT
EcoRI 838 GAATTC G^AATTC
1864 GAATTC
FauI 1341 GCGGG CCCGC(4/6)
1878 GCGGG
MflI 494 AGATCC [GA]^GATC[CT]
1584 GGATCT
MspA1I 761 CCGCTG C[AC]G^C[GT]G
1195 CAGCTG
NciI 1109 CCCGG CC^[GC]GG
1641 CCGGG
NdeI 1031 CATATG CA^TATG
1331 CATATG
NspI 559 ACATGC [GA]CATG^[CT]
997 GCATGC
SfaNI 286 GATGC GCATC(5/9)
857 GCATC
SmlI 1588 CTCAAG C^T[CT][GA]AG
1795 CTTGAG
XhoII 494 AGATCC [GA]^GATC[CT]
1584 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AvaII
BbvI BpmI BsaAI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfcI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI