Restriction map for
pFN21AE2668
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# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI Asp700I
AspEI AviII AvrII BamHI BbeI
BciVI BfrI BlnI BsaBI BsiEI
BsiWI BspLU11I BsrBI BsrFI BssHII
Bst1107I BstBI BstZ17I Cfr10I CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV EcoT22I FseI
FspI HaeII HindIII HpaI KasI
KspI MamI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SspI SwaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 1183 GTCTAC GT^[AC][GT]AC
AccIII 3572 TCCGGA T^CCGGA
AcyI 3818 GACGCC G[GA]^CG[CT]C
AflIII 200 ACGTGT A^C[GA][CT]GT
Alw44I 2845 GTGCAC G^TGCAC
AocI 239 CCTCAGG CC^T[ACGT]AGG
ApaI 957 GGGCCC GGGCC^C
ApaLI 2845 GTGCAC G^TGCAC
AspI 2998 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 454 CGAGACCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 74 TGATCA T^GATCA
BsaHI 3818 GACGCC G[GA]^CG[CT]C
BseAI 3572 TCCGGA T^CCGGA
BspDI 1780 ATCGAT AT^CGAT
BspEI 3572 TCCGGA T^CCGGA
BspMI 3029 ACCTGC ACCTGC(4/8)
BssSI 322 CACGAG CACGAG(-5/-1)
Bsu36I 239 CCTCAGG CC^T[ACGT]AGG
ClaI 1780 ATCGAT AT^CGAT
DraIII 3342 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 239 CCTCAGG CC^T[ACGT]AGG
EcoNI 3719 CCTACGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2749 GAATTC G^AATTC
FbaI 74 TGATCA T^GATCA
Hin1I 3818 GACGCC G[GA]^CG[CT]C
MroI 3572 TCCGGA T^CCGGA
NruI 1536 TCGCGA TCG^CGA
PflFI 2998 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2164 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 94 CAGCTG CAG^CTG
SapI 757 GAAGAGC GCTCTTC(1/4)
SbfI 3324 CCTGCAGG CCTGCA^GG
SmaI 722 CCCGGG CCC^GGG
SphI 2481 GCATGC GCATG^C
Sse8387I 3324 CCTGCAGG CCTGCA^GG
Tth111I 2998 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2164 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2229 TCTAGA T^CTAGA
XmaCI 722 CCCGGG C^CCGGG
XmaI 722 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccII 1537 CGCG CG^CG
3679 CGCG
AcsI 2749 GAATTC [GA]^AATT[CT]
3013 GAATTT
ApoI 2749 GAATTC [GA]^AATT[CT]
3013 GAATTT
AspHI 2845 GTGCAC G[AT]GC[AT]^C
3319 GTGCTC
BbrPI 199 CACGTG CAC^GTG
3345 CACGTG
BbvCI 1206 CCTCAGC CCTCAGC(-5/-2)
3801 CCTCAGC
BglI 1225 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1616 GCCTGGTGGGC
BglII 1962 AGATCT A^GATCT
2226 AGATCT
BsaAI 199 CACGTG [CT]AC^GT[GA]
3345 CACGTG
BsiHKAI 2845 GTGCAC G[AT]GC[AT]^C
3319 GTGCTC
BsmBI 1497 CGTCTC CGTCTC(1/5)
4036 CGTCTC
BsmI 894 GAATGC GAATGC(1/-1)
2040 GAATGC
BspHI 1908 TCATGA T^CATGA
2006 TCATGA
BsrDI 150 CATTGC GCAATG(2/0)
2618 GCAATG
BstEII 102 GGTCACC G^GT[ACGT]ACC
333 GGTCACC
BstPI 102 GGTCACC G^GT[ACGT]ACC
333 GGTCACC
BstUI 1537 CGCG CG^CG
3679 CGCG
BstXI 1022 CCATTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2699 CCACCCAAGTGG
EcoO65I 102 GGTCACC G^GT[ACGT]ACC
333 GGTCACC
HincII 2995 GTTGAC GT[CT]^[GA]AC
3025 GTTGAC
HindII 2995 GTTGAC GT[CT]^[GA]AC
3025 GTTGAC
MvnI 1537 CGCG CG^CG
3679 CGCG
NcoI 2541 CCATGG C^CATGG
2855 CCATGG
PaeR7I 1580 CTCGAG C^TCGAG
1834 CTCGAG
PmaCI 199 CACGTG CAC^GTG
3345 CACGTG
PmlI 199 CACGTG CAC^GTG
3345 CACGTG
PpuMI 1382 AGGTCCC [GA]G^G[AT]CC[CT]
2206 AGGACCT
RcaI 1908 TCATGA T^CATGA
2006 TCATGA
StuI 251 AGGCCT AGG^CCT
368 AGGCCT
XhoI 1580 CTCGAG C^TCGAG
1834 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
Acc65I AciI AfaI AluI AlwI
AlwNI Asp718I AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII