Restriction map for
pFN21AE2027
##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI Tth111I TthHB8I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AciI 921 CCGC CCGC(-3/-1)
AlwNI 1773 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1447 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1117 CCCGAG C^[CT]CG[GA]G
BalI 447 TGGCCA TGG^CCA
BamHI 1216 GGATCC G^GATCC
BbsI 1139 GTCTTC GAAGAC(2/6)
BcnI 1495 CCGGG CC^[GC]GG
BfaI 1145 CTAG C^TAG
BglII 338 AGATCT A^GATCT
BpuAI 1139 GTCTTC GAAGAC(2/6)
BsmI 1042 GAATGC GAATGC(1/-1)
BsoBI 1117 CCCGAG C^[CT]CG[GA]G
BspMI 641 ACCTGC ACCTGC(4/8)
BsrDI 710 GCAATG GCAATG(2/0)
BstAPI 1538 GCACTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 637 GGTTACC G^GT[ACGT]ACC
BstPI 637 GGTTACC G^GT[ACGT]ACC
BstXI 768 CCAAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1539 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 447 TGGCCA [CT]^GGCC[GA]
EcoO65I 637 GGTTACC G^GT[ACGT]ACC
EcoRI 1348 GAATTC G^AATTC
HapII 1495 CCGG C^CGG
HgaI 1572 GACGC GACGC(5/10)
HincII 229 GTCAAC GT[CT]^[GA]AC
HindII 229 GTCAAC GT[CT]^[GA]AC
HpaII 1495 CCGG C^CGG
MaeI 1145 CTAG C^TAG
MaeII 892 ACGT A^CGT
MluNI 447 TGGCCA TGG^CCA
MscI 447 TGGCCA TGG^CCA
MseI 47 TTAA T^TAA
MslI 30 CACTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1495 CCGG C^CGG
NciI 1495 CCGGG CC^[GC]GG
NcoI 787 CCATGG C^CATGG
PshAI 1613 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 137 ACCTGGT A^CC[AT]GGT
Tru9I 47 TTAA T^TAA
XmnI 1447 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 1348 GAATTC [GA]^AATT[CT]
1759 AAATTT
Alw44I 1098 GTGCAC G^TGCAC
1422 GTGCAC
ApaLI 1098 GTGCAC G^TGCAC
1422 GTGCAC
ApoI 1348 GAATTC [GA]^AATT[CT]
1759 AAATTT
AvaII 1027 GGACC G^G[AT]CC
1172 GGTCC
BsaBI 213 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
339 GATCTACATC
BsaI 466 GAGACC GGTCTC(1/5)
658 GAGACC
BseRI 1723 CTCCTC GAGGAG(10/8)
1384 GAGGAG
BsgI 387 GTGCAG GTGCAG(16/14)
948 GTGCAG
BsmAI 466 GAGAC GTCTC(1/5)
658 GAGAC
Bsp1407I 538 TGTACA T^GTACA
802 TGTACA
BspLU11I 1270 ACATGT A^CATGT
1597 ACATGT
BsrGI 538 TGTACA T^GTACA
802 TGTACA
BstYI 338 AGATCT [GA]^GATC[CT]
1216 GGATCC
EarI 160 GAAGAG CTCTTC(1/4)
1447 GAAGAG
EcoT14I 787 CCATGG C^C[AT][AT]GG
1667 CCTTGG
Ksp632I 160 GAAGAG CTCTTC(1/4)
1447 GAAGAG
MamI 213 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
339 GATCTACATC
MflI 338 AGATCT [GA]^GATC[CT]
1216 GGATCC
PflMI 281 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
742 CCAGCCAATGG
SspBI 538 TGTACA T^GTACA
802 TGTACA
StyI 787 CCATGG C^C[AT][AT]GG
1667 CCTTGG
Van91I 281 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
742 CCAGCCAATGG
XhoII 338 AGATCT [GA]^GATC[CT]
1216 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AfaI AflIII AluI AlwI AspHI
BanII BbvI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DsaI EcoRII
Fnu4HI FokI HaeIII HindIII HinfI
HphI ItaI MaeIII MboI MboII
MnlI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TfiI TseI
Tsp45I Tsp509I TspRI XcmI