Restriction map for
pFN21AB7761
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI AlwNI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BseAI BsgI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MboII MfeI MluI MroI
MseI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 364 GTCTAC GT^[AC][GT]AC
AccII 1287 CGCG CG^CG
AlwI 737 GGATC GGATC(4/5)
Asp700I 899 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 274 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 957 CTCGGG C^[CT]CG[GA]G
BalI 2 TGGCCA TGG^CCA
BbvI 359 GCTGC GCAGC(8/12)
BsaWI 684 ACCGGA [AT]^CCGG[AT]
BsmBI 677 GAGACG CGTCTC(1/5)
BsoBI 957 CTCGGG C^[CT]CG[GA]G
BsrFI 863 ACCGGC [GA]^CCGG[CT]
BstUI 1287 CGCG CG^CG
BstYI 737 GGATCT [GA]^GATC[CT]
Cfr10I 863 ACCGGC [GA]^CCGG[CT]
DraIII 508 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 828 CCATGG C^C[GA][CT]GG
EaeI 2 TGGCCA [CT]^GGCC[GA]
EcoRI 477 GAATTC G^AATTC
HgaI 362 GCGTC GACGC(5/10)
HincII 310 GTCAAC GT[CT]^[GA]AC
HindII 310 GTCAAC GT[CT]^[GA]AC
MflI 737 GGATCT [GA]^GATC[CT]
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MvnI 1287 CGCG CG^CG
NcoI 828 CCATGG C^CATGG
PflFI 274 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 531 CCATCGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 379 GACTC GAGTC(4/5)
PpuMI 960 GGGACCT [GA]G^G[AT]CC[CT]
SmlI 933 CTCAAG C^T[CT][GA]AG
TaqI 534 TCGA T^CGA
TfiI 158 GATTC G^A[AT]TC
TseI 359 GCTGC G^C[AT]GC
Tth111I 274 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 534 TCGA T^CGA
Van91I 531 CCATCGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 737 GGATCT [GA]^GATC[CT]
XmnI 899 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 477 GAATTC [GA]^AATT[CT]
918 GAATTT
Alw44I 762 GTGCAC G^TGCAC
1117 GTGCAC
ApaI 395 GGGCCC GGGCC^C
1045 GGGCCC
ApaLI 762 GTGCAC G^TGCAC
1117 GTGCAC
ApoI 477 GAATTC [GA]^AATT[CT]
918 GAATTT
BcgI 1195 GCACCCACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1267 GCAGTGGCCTCG
BcnI 375 CCCGG CC^[GC]GG
1110 CCCGG
BpmI 348 CTCCAG CTGGAG(16/14)
133 CTGGAG
BsaI 345 GGTCTC GGTCTC(1/5)
850 GGTCTC
BsmFI 960 GGGAC GGGAC(10/14)
280 GTCCC
BsrBI 794 CCGCTC CCGCTC(-3/-3)
1307 GAGCGG
BsrDI 1245 CATTGC GCAATG(2/0)
1079 GCAATG
DpnII 126 GATC ^GATC
738 GATC
EcoT14I 656 CCAAGG C^C[AT][AT]GG
828 CCATGG
Fnu4HI 359 GCTGC GC^[ACGT]GC
793 GCCGC
HinfI 158 GATTC G^A[ACGT]TC
379 GACTC
ItaI 359 GCTGC GC^[ACGT]GC
793 GCCGC
MaeII 308 ACGT A^CGT
680 ACGT
MboI 126 GATC ^GATC
738 GATC
NciI 375 CCCGG CC^[GC]GG
1110 CCCGG
NdeII 126 GATC ^GATC
738 GATC
NspI 708 GCATGT [GA]CATG^[CT]
890 ACATGC
Sau3AI 126 GATC ^GATC
738 GATC
StyI 656 CCAAGG C^C[AT][AT]GG
828 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanI BanII BmyI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DraII EcoO109I EcoRII
FauI FokI HaeIII HapII HpaII
HphI MaeIII MnlI MslI MspA1I
MspI MvaI MwoI NlaIII NlaIV
PvuII RsaI Sau96I ScrFI SfaNI
Tsp45I Tsp509I TspRI XcmI